REAL-TIME PCR

Real-Time PCR

RealQ Plus is a product range consisting of different 2x all-in-one master mixes intended for various qPCR applications.

RealQ Plus master mixes contain TEMPase Hot Start DNA Polymerase, dNTP’s, MgCl2 and a specialised buffer system necessary to perform optimal DNA amplification. The RealQ Plus master mixes display high efficiency as well as stability when working with gDNA and cDNA, which ensures the user precise and reliable quantification results as well as presence/absence results.

RealQ Plus 2x master mixes are produced in-house under ISO 9001:2015 conditions, ensuring that each batch possess the same robust performance every time.

  • RealQ Plus Master Mix Green and RealQ Plus Master Mix for Probe
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RealQ Plus 2x master mixes are available in two variants coming with three different levels of ROX™ reference dye. Use the ” Real-time PCR instrument guide” to select the correct level of ROX™ reference dye for your Real-time PCR experiments.

RealQ Plus Master Mix Green

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RealQ Plus Master Mix Green enables real-time-based DNA amplification with high specificity and efficiency. Real-time PCR is a sensitive and reliable method for gene analysis and DNA quantification. RealQ Plus 2x Master Mix Green is the right choice, when expenses and experiment preparation time might be limited or if you need to quickly analyse many genes.


FEATURES

  • Reliable master mix for real-time PCR
  • Based on DNA-binding fluorescent dye detection
  • Reliable quantification
  • High specificity, stability, reproducibility and efficiency
  • Reaction set-up at room temperature
  • Premixed all-in-one 2x solution

DESCRIPTION

RealQ Plus 2x Master Mix Green is composed of TEMPase Hot Start DNA Polymerase, dNTPs, fluorescent dye and an optimised buffer system. Furthermore, this master mix is available with high, low or without ROXTM ensuring optimal performance on most of the commonly used real-time PCR instruments. Just add DNA template and primers.

When the fluorescent Green dye is free in the solution, it emits a very low fluorescent signal. As soon as the dye binds to the double-stranded DNA the signal increases significantly (thousand fold), which makes the fluorescent signal of the dye directly proportional to the amount of amplified dsDNA.

SELECT THE CORRECT ROX LEVEL

Use the Real-time PCR instrument guide to find the correct level of ROX™ reference dye matching your Real-time PCR instrument.

HIGH EFFICIENCY AND SPECIFICITY

To examine the efficiency of the RealQ Plus 2x Master Mix Green, High ROXTM, a 4-fold dilution series with human gDNA was set up for PAH target (203 bp). Samples were made in triplicates starting with 80 ng down to 80 pg gDNA per well. The results depicted here shows high precision and efficiencies close to 100 %. The identical melt curves show a high specificity of the product and the standard curve shows linear detection range and a high accuracy of the TEMPase Hot Start DNA Polymerase

REPRODUCIBILITY

80 replicates of RealQ Plus 2x Master Mix Green, High ROX™ and 20 ng gDNA, show a standard deviation of only 0.084

STABILITY AT ROOM TEMPERATURE

Two plates were pre-assembled for qPCR reaction and incubated in darkness at room temperature for 48 and 72 hours. The results show high stability and complete inactivation of the TEMPase before hot start. It allows the scientist to set up the reaction and run the plate several hours later, when convenient

PRODUCT INFO

RealQ Plus Master Mix Green

Green without ROX

Sample

Green with low ROX

Sample

Green with high ROX

Sample

 

RealQ Plus Master Mix for Probe

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RealQ Plus Master Mix for Probe enables real-time-based DNA amplification with high specificity and efficiency. Real-time PCR is a sensitive and reliable method for gene analysis and DNA quantification. RealQ Plus 2x Master Mix for Probe is the right choice, when expenses and experiment preparation time might be limited or if you need to quickly analyse many genes.

 

FEATURES

  • Promotes probe-based real-time PCR based detection
  • Enables multiplexing
  • Very high specificity
  • High stability and efficiency
  • Reliable quantification
  • Reaction set-up at room temperature
  • Premixed all-in-one 2x solution

DESCRIPTION

RealQ Plus 2x Master Mix for Probe is composed of TEMPase Hot Start DNA Polymerase, dNTPs, dye and an optimised buffer system. Furthermore, this master mix is available with high, low or without ROXTM ensuring optimal performance on most of the commonly used real-time PCR instruments. Just add DNA template, primers and probe.

The RealQ Plus 2x Master Mix for probe is optimised to TaqMan probes, but is also suitable with other probe chemistries such as Molecular Beacon and Scorpion. 

Most probe-based detection methods take advantage of fluorescent resonance energy transfer (FRET) by quenching the signal of a fluorescent reporter in the absence of the desired target. During elongation, the quenching molecule is separated from the fluorescent reporter and a signal is emitted and monitored. Probe-based detection is significantly more specific since signal is only detected when the correct target is amplified.

SELECT THE CORRECT ROX LEVEL

Use the Real-time PCR instrument guide to find the correct level of ROX™ reference dye matching your Real-time PCR instrument.

HIGH EFFICIENCY AND PRECISION

Amplification plot of a 4-fold dilution series for a Pthr target (75 bp) amplified from human gDNA, starting with 80 ng down to 80 pg of gDNA, using RealQ Plus 2x Master Mix for Probe, High ROX™. Samples were made in triplicates. The results depicted here, show high precision and efficiencies close to 100 %

REPRODUCIBILITY

80 replicates of RealQ Plus 2x Master Mix Green, High ROX™ and 20 ng gDNA, show a standard deviation of only 0.084.

STABILITY AT ROOM TEMPERATURE

Two plates were pre-assembled for qPCR reaction and incubated in darkness at room temperature for 48 and 72 hours. The results show high stability and complete inactivation of the TEMPase before hot start. It allows the scientist to set up the reaction and run the plate several hours later, when convenient.

PRODUCT INFO

PRODUCT INFO

RealQ Plus Master Mix for Probe

For Probe without ROX
Sample
For Probe with low ROX
Sample
For Probe with high ROX
Sample
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