GENOTYPING

Genotyping

Genotyping is a technology used for detection of minor genetic differences. Genotyping determines differences in genetic material by comparing one DNA sequence to another DNA sequence or a reference. Genotyping is used in research, diagnostic and medicine.

Q-Extract DNA Extraction PCR Kit is ideal for genotyping. The kit consists of Q-Extract DNA Extraction Solution and Taq DNA Polymerase 2x Master Mix RED.

Q-Extract DNA Extraction Solution is designed for rapid and efficient extraction of PCR-ready DNA from various sample types e.g. mouse tails, mouse ear snips, other mammalian tissues/organs, saliva and bacteria. The included Taq DNA Polymerase 2x Master Mix is a ready-to-use reaction solution to perform PCR. The red dye and stabilizer within the master mix formulation enables the user to load amplified PCR samples directly to the DNA gel. 

Q-Extract DNA Extraction PCR Kit

The Q-Extract DNA Extraction PCR Kit is ideal for genotyping. It offers rapid and efficient DNA extraction and amplification of a broad range of mammalian tissues.

DNA extraction from various mammalian tissues and bacteria in just 8 minutes is followed by PCR amplification by Taq DNA Polymerase 2x Master Mix RED


FEATURES

  • Ideal solution for genotyping
  • Rapid 8-minute extraction protocol
  • Minimal handling
  • DNA extracts from mammalian tissues and bacteria
  • PCR-ready DNA
  • Reliable PCR results
  • Direct gel loading
  • Scalable set-up
  • Automation-friendly

DESCRIPTION

The Q-Extract DNA Extraction PCR Kit consists of Q-Extract DNA extraction solution and Taq DNA Polymerase 2x Master Mix RED.

The non-toxic Q-Extract DNA Extraction Solution is optimized for easy extraction of PCR-ready DNA. DNA can be extracted from various mammalian tissues e.g. mouse tails, ear snips, liver, kidney, lung and saliva.  The one-step lysis is performed in ether a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is ready for PCR without further handling such as vortex, centrifugation or dilutions.

The extracted DNA is amplified using the user-friendly Taq DNA Polymerase 2x Master Mix RED for PCR.

Taq DNA Polymerase 2x Master Mix is a ready-to-use reaction solution. The red dye and stabilizer within the master mix formulation enables the user to load amplified PCR samples directly to the DNA gel. The red dye also provides visualization of pipetting and mixing

The obtained DNA extract is stable at – 20°C for up to one week and at – 80°C for long term storage.

The Q-Extract DNA Extraction Solution is also available in a format without Taq DNA Polymerase 2x Master Mix RED.

EFFICIENT AMPLIFICATION OF DNA EXTRACTED FROM VARIOUS MAMMALIAN TISSUES

Q-Extract DNA Extraction PCR Kit was used to extract and amplify genomic DNA from various mammalian tissues. For DNA extraction, 0.5 -10 mg tissue or 20 µl salvia was added to100 µl of Q-Extract DNA Extraction Solution. M: DNA marker Iqon Low DNA Ladder. Lane 1-5: Different mouse tissues as depicted, GADPH (266 bp). Lane 6: Chicken muscle tissue, HRPT1 (245 bp) and Lane 7: Human saliva, DMD17 (415 bp).

PERFORMANCE OF Q-EXTRACT DNA EXTRACTION KIT COMPARED TO AN EQUIVALENT PCR KIT FOR GENOTYPING

The performance of Q-Extract DNA Extraction PCR Kit was compared to the genotyping PCR kit from competitor B. DNA extracts were extracted from chicken muscle (lanes 1-2) and mouse tail (lanes 3-4). Each extraction and amplification have been conducted according to the supplier manuals and using the PCR master mixes provided in the respective kits. The result shows that the Q-Extract DNA Extraction PCR Kit and the competitor B performs equally well. The extraction protocol from supplier B is less user-friendly, takes double time and requires more handling. M: DNA marker Iqon Low DNA Ladder. Lane 1: Chicken HRTP1 (245 bp), Lane 2: Chicken GADPH (775 bp), Lane 3: Mouse GADPH (265 bp) and Lane 4: Mouse B-actin (318 bp).

PRODUCT INFO

Q-Extract DNA Extraction PCR Kit

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