TAQ DNA POLYMERASE
Ampliqon Taq DNA Polymerase is a recombinant thermostable DNA polymerase originating from the thermophilic bacterium Thermus aquaticus.
Ampliqon Taq Polymerase is manufactured in accordance with our ISO 9001:2015 quality management system, ensuring that each batch possess the same robust performance every time. Ampliqon Taq DNA Polymerase is the perfect choice for routine PCR applications which require high yield and reliable PCR amplification.
Taq DNA Polymerase, Taq DNA Polymerase RED, Taq OptiMix CLEAR, Taq DNA Polymerase Master Mix RED & Taq DNA Polymerase Master Mix
Ampliqon Taq DNA polymerase is available in different standalone variants, concentrations and master mixes.
Standalone variants are supplied either without buffers or in kits including MgCl2 and one or two Ampliqon PCR buffers to achieve highest PCR performance and to avoid tedious reaction optimisation.
Master mixes include all components required for high performance PCR, just add your specific primers and DNA template.
Taq DNA Polymerase
Ampliqon Taq DNA Polymerase 5 U/µl is ideal for routine PCR applications requiring high yield and also reliable DNA amplification. Taq DNA Polymerase 5 U/µl is available in different sizes.
FEATURES
- A thermostable recombinant DNA Polymerase
- High product yield
- dUTP incorporation possible
- processes up to 5 kb
- Leaves a 3’dA overhang
DESCRIPTION
This has a molecular weight of 95 kDa and exhibits both a 5’→3′ DNA polymerase and a 5’→3 exonuclease activity. The 5′-3′ exonuclease activity leaves a 3’dA overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase lacks 3′-5′ exonuclease activity and proofreading ability.
Taq DNA polymerase is suitable for standard testing, routine PCR, screening and high throughput testing.
Taq DNA polymerase also exits in glycerol format for automation and lyophilization: Taq DNA Polymerase Glycerol Free
Ampliqon Taq DNA Polymerase was compared with a Taq DNA Polymerase from a well-recognised competitor. Three different DNA target varying in length PAH (203 bp), ENG9 (293 bp) and ENG 5 (480 bp) were evaluated. Ampliqon Taq DNA Polymerase performed equally well or better in this study. Furthermore, Ampliqon Taq DNA Polymerase displays a higher specificity on the PAH and ENG5 targets, than the competing Taq DNA Polymerase. Each Amplification has been conducted according to supplier’s manual.
Taq DNA Polymerase RED
Taq DNA Polymerase RED 5 U/µl is suitable for routine PCR applications requiring high yield and also reliable DNA amplification. Taq DNA Polymerase RED is a good alternative to Ampliqon Taq DNA Polymerase. The red dye in this product provides convenient identification of enzyme addition to the tube and also confirms complete mixing. Taq DNA Polymerase RED is available in different sizes.
FEATURES
- Easy identification of enzyme addition
- Confirmation of complete mixing
- High product yield
- Processes up to 5 kb
- dUTP incorporation possible
- Leaves a 3’dA overhang
DESCRIPTION
The inert red dye has no effect on downstream processes but adds visibility to the enzyme. This makes Taq DNA Polymerase RED very useful for high throughput testing. Taq DNA Polymerase RED is added directly to the PCR reaction mix.
VISUALISATION OF COMPLETE MIXING USING TAQ DNA POLYMERASE RED
Taq OptiMix CLEAR
Taq OptiMix CLEAR
Taq OptiMix CLEAR is an optimised format of Taq DNA Polymerase Master Mix and is therefore a good alternative to Taq DNA Polymerase and Taq DNA Polymerase Master Mix. Taq OptiMix CLEAR ensures the user an increased specificity and improved PCR performance, even on difficult templates. Taq OptiMix CLEAR offers reduced set-up time and eliminates the risk of contamination of reaction components. Taq OptiMix CLEAR is suitable for standard PCR applications, screening and high throughput testing.
FEATURES
- All-in-one 2x master mix for great convenience
- Timesaving reaction setup
- Increased specificity compared to Taq DNA Polymerase Master Mix
DESCRIPTION
Taq OptiMix CLEAR is a ready to use 2x reaction mix. Taq OptiMix CLEAR is composed of Ampliqon Taq DNA polymerase and an optimised buffer system including dNTPs and magnesium chloride
COMPARISON BETWEEN TAQ OPTIMIX CLEAR AND THREE COMPETITORS
Taq DNA Polymerase Master Mix RED
This is a very popular alternative to Taq DNA Polymerase Master Mix with the same advantages. The inert red dye and stabiliser are extra features, which allow direct loading onto your agarose gel for analysis.
FEATURES
- Premixed solution
- Minimal optimisation
- Direct loading onto agarose and SDS DNA gels
- Easy visualisation of pipetting
- Dye front runs at 300 – 1000 bp on 0.5 – 1.5 % agarose gel
- No need for a separate loading dye
- Increased reproducibility
- High product yield
- dUTP incorporation possible
- Processes up to 5 kb
- Leaves a 3’dA overhang
DESCRIPTION
The Master Mix RED is a ready to use 1.1x or 2x master mix. Taq DNA Polymerase Master Mix RED is available in two final MgCl2 concentrations: 1.5 mM and 2.0 mM. Taq DNA Polymerase Master Mix RED is composed of Ampliqon Taq DNA Polymerase, the NH4+ buffer system, dNTPs and magnesium chloride.
The red dye and the stabiliser do not interfere with the PCR. If necessary, the red dye can be removed from the PCR product by spin column purification or other methods. Furthermore, PCR products generated using Taq DNA Polymerase Master Mix RED DNA, can directly be used for sequencing after treatment with either spin columns or PureIT ExoZAP PCR CleanUp.
DIRECT GEL LOADING
COMPARISON BETWEEN TAQ OPTIMIX CLEAR AND THREE COMPETITORS
COMPARISON BETWEEN TAQ DNA POLYMERASE MASTER MIX RED AND TWO COMPETITORS
RED DYE EXHIBITS NO PCR INTERFERENCE
Taq DNA Polymerase Master Mix
This is a good alternative to Ampliqon Taq DNA Polymerase. Taq DNA Master Mix offers reduced set-up time and eliminates the risk of contamination of reaction components. Fewer handling steps lead to better reproducibility, which makes Taq DNA Polymerase Master Mix suitable for standard PCR applications, screening and high throughput testing.
FEATURES
- Premixed solution
- Minimal optimisation
- Fewer pipetting steps
- Increased reproducibility
- High product yield
- dUTP incorporation possible
- Processes up to 5 kb
- Leaves a 3’dA overhang
DESCRIPTION
Taq DNA Polymerase Master Mix is a ready to use 1.1x or 2x reaction mix. Taq DNA Polymerase Master Mix is composed of Ampliqon Taq DNA Polymerase, the
NH4+ buffer system, dNTPs and magnesium chloride.
COMPARISON BETWEEN TAQ DNA POLYMERASE MASTER MIX AND A COMPETITOR
