TAQ DNA POLYMERASE

  • PCR Enzymes
  • Taq
  • Glycerol Free Products
  • Hot Start
  • High Fidelity
  • Genotyping
  • Multiplex
  • GC-rich DNA Amplification
  • Real-Time PCR
  • HRM - High Resolution Melting
  • Nucleotides
  • PCR Buffers
  • DNA Loading Buffer
  • PCR Ladders
  • DNA and RNA Extraction
  • PCR Clean-Up

TAQ DNA POLYMERASE

Ampliqon Taq DNA Polymerase is a recombinant thermostable DNA polymerase originating from the thermophilic bacterium Thermus aquaticus.

Ampliqon Taq Polymerase is manufactured in accordance with our ISO 9001:2015 quality management system, ensuring that each batch possess the same robust performance every time. Ampliqon Taq DNA Polymerase is the perfect choice for routine PCR applications which require high yield and reliable PCR amplification.

Taq DNA Polymerase, Taq DNA Polymerase RED, Taq OptiMix CLEAR, Taq DNA Polymerase Master Mix RED & Taq DNA Polymerase Master Mix

Ampliqon Taq DNA polymerase is available in different standalone variants, concentrations and master mixes.

Standalone variants are supplied either without buffers or in kits including MgCl2 and one or two Ampliqon PCR buffers to achieve highest PCR performance and to avoid tedious reaction optimisation.

Master mixes include all components required for high performance PCR, just add your specific primers and DNA template.

Taq DNA Polymerase

Ampliqon Taq DNA Polymerase 5 U/µl  is ideal for routine PCR applications requiring high yield and also reliable DNA amplification. Taq DNA Polymerase 5 U/µl  is available in different sizes.

FEATURES

  • A thermostable recombinant DNA Polymerase
  • High product yield
  • dUTP incorporation possible
  • processes up to 5 kb
  • Leaves a 3’dA overhang

DESCRIPTION

This has a molecular weight of 95 kDa and exhibits both a 5’→3′ DNA polymerase and a 5’→3 exonuclease activity. The 5′-3′ exonuclease activity  leaves a 3’dA overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase lacks 3′-5′ exonuclease activity and proofreading ability.

Taq DNA polymerase is suitable for standard testing, routine PCR, screening and high throughput testing.

Taq DNA polymerase also exits in glycerol format for automation and lyophilization: Taq DNA Polymerase Glycerol Free

Ampliqon Taq DNA Polymerase was compared with a Taq DNA Polymerase from a well-recognised competitor. Three different DNA target varying in length PAH (203 bp), ENG9 (293 bp) and ENG 5 (480 bp) were evaluated. Ampliqon Taq DNA Polymerase performed equally well or better in this study. Furthermore, Ampliqon Taq DNA Polymerase displays a higher specificity on the PAH and ENG5 targets, than the competing Taq DNA Polymerase. Each Amplification has been conducted according to supplier’s manual.

PRODUCT INFO

Taq DNA Polymerase

With Ammonium Buffer, 5 U/µl

With Standard Buffer, 5 U/µl

With Combination Buffer, 5 U/µl

5x PCR Buffer RED, 5 U/µl

Without buffer, 5 U/µl

Sample, 5 U/µl

Taq DNA Polymerase RED

Taq DNA Polymerase RED 5 U/µl is suitable for routine PCR applications requiring high yield and also reliable DNA amplification. Taq DNA Polymerase RED is a good alternative to Ampliqon Taq DNA Polymerase. The red dye in this product provides convenient identification of enzyme addition to the tube and also confirms complete mixing. Taq DNA Polymerase RED is available in different sizes.

FEATURES

  • Easy identification of enzyme addition
  • Confirmation of complete mixing
  • High product yield
  • Processes up to 5 kb
  • dUTP incorporation possible
  • Leaves a 3’dA overhang

DESCRIPTION

The inert red dye has no effect on downstream processes but adds visibility to the enzyme. This makes Taq DNA Polymerase RED very useful for high throughput testing. Taq DNA Polymerase RED is added directly to the PCR reaction mix.

VISUALISATION OF COMPLETE MIXING USING TAQ DNA POLYMERASE RED

PRODUCT INFO

Taq DNA Polymerase RED

With Ammonium Buffer, 5 U/µl
With Standard Buffer, 5 U/µl
With Combination Buffer, 5 U/µl
Without buffer, 5 U/µl
Sample, 5 U/µl

Taq OptiMix CLEAR

Taq OptiMix CLEAR

Taq OptiMix CLEAR is an optimised format of Taq DNA Polymerase Master Mix and is therefore a good alternative to Taq DNA Polymerase and Taq DNA Polymerase Master Mix.  Taq OptiMix CLEAR ensures the user an increased specificity and improved PCR performance, even on difficult templates. Taq OptiMix CLEAR offers reduced set-up time and eliminates the risk of contamination of reaction components. Taq OptiMix CLEAR is suitable for standard PCR applications, screening and high throughput testing.

 

FEATURES

  • All-in-one 2x master mix for great convenience
  • Timesaving reaction setup
  • Increased specificity compared to Taq DNA Polymerase Master Mix

DESCRIPTION

Taq OptiMix CLEAR is a ready to use 2x reaction mix. Taq OptiMix CLEAR is composed of Ampliqon Taq DNA polymerase and an optimised buffer system including dNTPs and magnesium chloride

COMPARISON BETWEEN TAQ OPTIMIX CLEAR AND THREE COMPETITORS

Taq OptiMix CLEAR was compared with corresponding Taq DNA Polymerase master mixes from three well recognised competitors; T,K and N. Six different primer targets (BAIP, Q8, CFTR, ENG5, ENG9 and PAH) were evaluated. Taq OptMix CLEAR performed equally well or better on all six primer targets tested in this set up. Each amplification was conducted according to supplier’s manual.

PRODUCT INFO

Taq OptiMix CLEAR

Product number
Sample

Taq DNA Polymerase Master Mix RED

This is a very popular alternative to Taq DNA Polymerase Master Mix with the same advantages. The inert red dye and stabiliser are extra features, which allow direct loading onto your agarose gel for analysis.

FEATURES

  • Premixed solution
  • Minimal optimisation
  • Direct loading onto agarose and SDS DNA gels
  • Easy visualisation of pipetting
  • Dye front runs at 300 – 1000 bp on 0.5 – 1.5 % agarose gel
  • No need for a separate loading dye
  • Increased reproducibility
  • High product yield
  • dUTP incorporation possible
  • Processes up to 5 kb
  • Leaves a 3’dA overhang

DESCRIPTION

The Master Mix RED is a ready to use 1.1x or 2x master mix. Taq DNA Polymerase Master Mix RED is available in two final MgClconcentrations: 1.5 mM and 2.0 mM. Taq DNA Polymerase Master Mix RED is composed of Ampliqon Taq DNA Polymerase, the NH4+ buffer system, dNTPs and magnesium chloride.

The red dye and the stabiliser do not interfere with the PCR. If necessary, the red dye can be removed from the PCR product by spin column purification or other methods. Furthermore, PCR products generated using Taq DNA Polymerase Master Mix RED DNA, can directly be used for sequencing after treatment with either spin columns or PureIT ExoZAP PCR CleanUp. 

DIRECT GEL LOADING

After end of amplification PCR products can be loaded directly onto agarose and SDS DNA gels. The red dye provides easy visualisation of both pipetting and gel loading. The red dye front runs at 300 – 1000 bp on 0.5 – 1.5 % agarose gels.

COMPARISON BETWEEN TAQ OPTIMIX CLEAR AND THREE COMPETITORS

COMPARISON BETWEEN TAQ DNA POLYMERASE MASTER MIX RED AND TWO COMPETITORS

Ampliqon Taq DNA Polymerase Master Mix RED was compared with a Taq DNA Polymerase master mix from two well recognised competitors. Four different DNA target varying in length PAH (203 bp), Q8 (727 bp), CFTR (613 bp) and BAIP (788) were evaluated. Ampliqon Taq DNA Polymerase Master Mix RED performed equally well or better in this study. Each Amplification has been conducted according to suppliers manual.
 

RED DYE EXHIBITS NO PCR INTERFERENCE

The red dye does not interfere with PCR performance. Two different DNA targets; PAH (203 bp) and BAIP (788 bp) were amplified in duplicates using Taq Master Mix RED and visualised on a agarose gel.

PRODUCT INFO

Taq DNA Polymerase Master Mix RED

2x and 1.5 mM MgCl2
Sample
2x and 2.0 mM MgCl2
Sample
1.1x and 1.5 MgCl2
Sample
1.1x and 2.0 mM MgCl2
Sample

Taq DNA Polymerase Master Mix

This is a good alternative to Ampliqon Taq DNA Polymerase. Taq DNA Master Mix offers reduced set-up time and eliminates the risk of contamination of reaction components. Fewer handling steps lead to better reproducibility, which makes Taq DNA Polymerase Master Mix suitable for standard PCR applications, screening and high throughput testing.

FEATURES

  • Premixed solution
  • Minimal optimisation
  • Fewer pipetting steps
  • Increased reproducibility
  • High product yield
  • dUTP incorporation possible
  • Processes up to 5 kb
  • Leaves a 3’dA overhang

DESCRIPTION

Taq DNA Polymerase Master Mix is a ready to use 1.1x or 2x reaction mix. Taq DNA Polymerase Master Mix is composed of Ampliqon Taq DNA Polymerase, the

NH4buffer system, dNTPs and magnesium chloride.

COMPARISON BETWEEN TAQ DNA POLYMERASE MASTER MIX AND A COMPETITOR

Ampliqon Taq DNA Polymerase Master Mix was compared with a similar Taq DNA Polymerase master mix from a well-recognised competitor. Three different DNA target varying in length PAH (203 bp), Q8 (727 bp) and CFTR (613 bp) were evaluated. Ampliqon Taq DNA Polymerase Master Mix performed equally well or better in this study. Each Amplification has been conducted according to supplier’s manual.

PRODUCT INFO

Taq DNA Polymerase Master Mix

1.1x and 1.5 MgCl2
Sample
1.1x and 2.0 mM MgCl2
Sample
2x and 1.5 mM MgCl2
Sample
2x and 2.0 mM MgCl2
Sample