HIGH FIDELITY DNA POLYMERASE

High Fidelity

AQ90 High Fidelity DNA Polymerase from Ampliqon is a proofreading DNA polymerase with the ability to perform robust amplification of a vast range of difficult targets, including those up to 12.5 kb and with high to low GC content. The fidelity of the polymerase has been measured up 50x Taq DNA Polymerase.

AQ90 High Fidelity DNA Polymerase is available in two variants; as 2x Master Mix or as standalone supplied with 10x AQ90 Buffer in kits including MgCl2 for highest PCR performance and to avoid tedious reaction optimisation. Amplification is supported by an optimized PCR buffer system allowing robust amplification on DNA targets with low to high GC content and long DNA targets. 

AccuPOL DNA Polymerase is a thermostable high fidelity DNA polymerase with proofreading ability. AccPOL DNA polymerase is available in different standalone variants supplied either without buffers or in kits including MgCl2 and Ampliqon PCR buffer for highest PCR performance and without tedious reaction optimisation.

The combination of AccuPOL DNA Polymerase + Ammonium Buffer ensures the highest PCR performance without tedious reaction optimisation

AQ90 High Fidelity DNA Polymerase and AccuPOL is manufactured in-house in accordance to our ISO 9001:2015 quality management system, ensuring that each batch possess the same robust performance every time

AQ90 High Fidelity DNA Polymerase

AQ90 High Fidelity DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity is measured up to 50x Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets. These feature enables accurate and reliable PCR results.

AQ90 High Fidelity DNA Polymerase is recommended for applications, which require extremely high fidelity such as cloning/sub-cloning, NGS applications and mutagenesis.

FEATURES

  • High fidelity – measured up to 50x Taq Fidelity
  • 3’→5’ proofreading exonuclease activity
  • Exceptional low error rate
  • Processes up to 8.5 kb gDNA and ≤ 12.5 kb for λDNA
  • Good coverage on DNA templates with low to high GC content

DESCRIPTION

AQ90 High Fidelity DNA Polymerase exhibits both 5’→3′ DNA polymerase activity and 3’→5′ proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.

AQ90 High Fidelity DNA Polymerase kit is supported with 10x AQ90 Buffer allowing robust amplification on DNA targets with low to high GC content and long DNA targets. The kit also includes an extra tube of MgCl2 in 25 mM concentration if adjustment of PCR conditions is required.

For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.

For more convenient handling AQ90 High Fidelity DNA Polymerase is also available as a 2x master mix

FIDELITY

The fidelity of a polymerase refers to its ability to insert the correct base during PCR. On the contrary, the rate of misincorporation is known as a polymerases error rate.  Fidelity depends on the polymerase, the buffer system in use and the quality of your template DNA.

AQ90 PROMOTES ROBUST AMPLIFICATION

Performance of AQ90 High Fidelity DNA Polymerase was compared to three leading High Fidelity DNA Polymerases (A, B and C). Nine different human genomic DNA targets, 400 – 800 bp in length and with GC content ranging from 29 – 78 %, were amplified. Robust amplification was observed using AQ90 High Fidelity DNA Polymerase on the nine human genomic targets. In contrary, none of the three leading High Fidelity DNA polymerase were able to provide the same level of robust amplification on the DNA targets with the higher GC content, under the conditions tested here. Amplification studies has been set up, as recommended by the manufactures. Tm Calculators of the respective competitors were used to calculate optimal annealing temperature for primers. When amplifying GC-rich targets, 2 M Betaine Enhancer Solution (AQ90 DNA Polymerase), GC enhancer (Competitor A and B) or GC-rich specific PCR Buffer (competitor C) were included in the reaction mix.

PRODUCT INFO

AQ90 High Fidelity DNA Polymerase

With 10x AQ90 Buffer, 2 U/µl
Sample, 2 U/µl

AQ90 High Fidelity DNA Polymerase Master Mix

AQ90 High Fidelity DNA Polymerase 2x Master Mix is a convenient alternative to AQ90 High Fidelity DNA Polymerase, displaying the following features; high fidelity – measured up to 50x Taq DNA Polymerase, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification on long DNA targets.

AQ90 High Fidelity DNA Polymerase 2x Master Mix is recommended for applications, which require extremely high fidelity such as cloning/Sub-cloning, NGS applications and mutagenesis.


FEATURES

  • All-in-one 2x master mix for great convenience
  • Timesaving reaction set-up
  • High fidelity – measured up to 50x Taq Fidelity
  • 3’→5’ proofreading exonuclease activity
  • Exceptional low error rate
  • Processes up to 8.5 kb gDNA and ≤ 12kb for λDNA
  • Good coverage on DNA templates with low to high GC content

DESCRIPTION

AQ90 High Fidelity DNA Polymerase 2x Master Mix is a ready to use 2x reaction mix composed of AQ90 High Fidelity DNA Polymerase and an optimised buffer system including dNTPs and magnesium chloride, allowing robust amplification on DNA target with low to high GC content and long DNA targets.

AQ90 High Fidelity DNA Polymerase exhibits both 5’→3′ DNA polymerase activity and 3’→5′ proofreading exonuclease activity enabling this polymerase to correct base pair mismatches.

For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or long amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.

FIDELITY

The fidelity of a polymerase refers to its ability to insert the correct base during PCR. On the contrary, the rate of misincorporation is known as a polymerases error rate.  Fidelity depends on the polymerase, the buffer system in use and the quality of your template DNA.

AQ90 2X MASTER MIX PROMOTES ROBUST AMPLIFICATION

AQ90 High Fidelity DNA Polymerase and AQ90 High Fidelity 2x Master Mix promotes specific and clear results on a variety of DNA targets with low to high GC content. Here we have tested 9 different DNA targets varying in both length; ranging from 200 bp to above 700 bp and in GC content; ranging from 29 – 78 %. Amplification of targets with 71 – 78 % GC content was only successful when Betaine Enhancer Solution (2

LONG RANGE AMPLIFICATION

AQ90 High Fidelity DNA Polymerase 2x Master Mix enables amplification of large and complex amplicons. Four different targets of human genomic DNA ranging from 2 kb and up to 8.5 kb was used in this study. Amplicon sizes are indicated at the top of the gel. Marker M is High Range DNA Ladder from Ampliqon (A610141)

PRODUCT INFO

AQ90 High Fidelity DNA Polymerase Master Mix

2x and 2.0 mM MgCl2
Sample

AccuPOL DNA Polymerase

AccuPOL DNA Polymerase 2.5 U/µl is a thermostable high fidelity DNA polymerase with proofreading ability. This feature enables accurate and reliable PCR results.

AccuPOL DNA Polymerase is recommended for applications, which require extremely high fidelity or blunt ends. This polymerase is very suitable for mutation studies, cloning, gene expression and construction of libraries.


FEATURES

  • High fidelity enzyme
  • Proof reading
  • Error rate 1.1 x 10-6
  • Processes up to 3 kb
  • Renders blunt-ended DNA

DESCRIPTION

AccuPOL DNA Polymerase exhibits both 5’→3′ DNA polymerase activity and 3’→5′ proofreading exonuclease activity enabling this polymerase to correct base pair mismatches. All AccuPOL kits include an extra tube of MgCl2 in 25 mM concentration if adjustment of PCR conditions is required.

AccuPOL DNA Polymerase also comes in an alternative Tween free version. 

FIDELITY

Fidelity depends on the polymerase, the buffer system in use and the quality of your template DNA.

ACCUPOL DNA POLYMERASE PROMOTES SPECIFIC AND CLEAR RESULTS

AccuPOL DNA Polymerase promotes specific and clear results on a variety of DNA targets. Here we have tested 6 different DNA target varying in both length; ranging from 200 bp to above 3.000 bp and in GC content; ranging from 30 – 65 %.

PRODUCT INFO

AccuPOL DNA Polymerase

With Ammonium Buffer, 2.5 U/µl
Without buffer, 2.5 U/µl
Sample, 2.5 U/µl
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