Glycerol Free Products
Ampliqon offers glycerol free products:
TEMPase Hot Start DNA Polymerase Glycerol Free and Taq DNA Polymerase Glycerol Free
Ampliqon Taq DNA Polymerase Glycerol Free and TEMPase Hot Start DNA Polymerase Glycerol Free are a glycerol free formulation of the respective stand alone DNA polymerases.
The glycerol free products are manufactured in-house in accordance to our ISO 9001:2015 quality management system, ensuring that each batch possess the same robust performance every time.
WHAT GLYCEROL DOES
Glycerol is normally a major part of the storage buffer for enzymes, and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, thus stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.
Taq DNA Polymerase Glycerol Free
Taq DNA Polymerase Glycerol Free is a glycerol free formulation of our regular Taq DNA Polymerase and is well suited for automated high throughput testing, lyophilization or where accurate pipetting of small volumes are crucial. Taq DNA Polymerase Glycerol Free is available in different sizes and two formats; 5 U/µl and as highly concentrated 50 U/µl.
FEATURES
- Glycerol free storage buffer
- Suitable for automated high throughput testing
- Suitable for applications involving freeze drying
- Suitable for robot-aided pipetting
- High product yield
- Processes up to 5 kb
- dUTP incorporation possible
- Leaves a 3’dA overhang
DESCRIPTION
Ampliqon Taq DNA Polymerase Glycerol Free exhibits both a 5’→3’dA DNA polymerase and a 5’→3′ exonuclease activity. The 5′-3′ exonuclease activity leaves a 3′ overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase Glycerol Free lacks 3′-5′ exonuclease activity and proofreading ability.
WHAT GLYCEROL DOES
Glycerol is normally a major part of the storage buffer for enzymes, and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, thus stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.
TAQ DNA POLYMERASE GLYCEROL FREE FOR AUTOMATION AND FREEZE DRYING
TEMPase Hot Start DNA Polymerase Glycerol Free
TEMPase Hot Start DNA Polymerase Glycerol Free 5 U/µl is a glycerol free formulation of our regular TEMPase Hot Start DNA Polymerase 5 U/µl and is well suited for automated high throughput testing, freeze drying or where accurate pipetting of small volumes are crucial
FEATURES
- Convenient reaction set up at room temperature
- Automated high throughput testing
- Freeze drying
- dUTP incorporation possible
- Increased sensitivity, specificity and product yield
DESCRIPTION
TEMPase Hot Start DNA Polymerase Glycerol Free 5 U/µl is a chemically modified version of Ampliqon Taq DNA Polymerase and is activated by heat treatment.
A chemical moiety is attached to the enzyme at the active site, which renders the TEMPase Hot Start enzyme inactive at room temperature.
During set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the PCR reaction reaches the optimal activation temperature, the chemical moiety is cleaved releasing the active TEMPase Hot Start DNA Polymerase, resulting in higher specificity, sensitivity and yield compared to standard Ampliqon Taq DNA polymerase.
Glycerol is normally a major part of the storage buffer of enzymes and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, hence stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast, robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.
GLYCEROL FREE FOR AUTOMATION AND FREEZE DRYING
