Genotyping
Genotyping is a technology used for detection of minor genetic differences. Genotyping determines differences in genetic material by comparing one DNA sequence to another DNA sequence or a reference. Genotyping is used in research, diagnostic and medicine.
Q-Extract DNA Extraction PCR Kit is ideal for genotyping. The kit consists of Q-Extract DNA Extraction Solution and Taq DNA Polymerase 2x Master Mix RED.
Q-Extract DNA Extraction Solution is designed for rapid and efficient extraction of PCR-ready DNA from various sample types e.g. mouse tails, mouse ear snips, other mammalian tissues/organs, saliva and bacteria. The included Taq DNA Polymerase 2x Master Mix is a ready-to-use reaction solution to perform PCR. The red dye and stabilizer within the master mix formulation enables the user to load amplified PCR samples directly to the DNA gel.
Q-Extract DNA Extraction PCR Kit
The Q-Extract DNA Extraction PCR Kit is ideal for genotyping. It offers rapid and efficient DNA extraction and amplification of a broad range of mammalian tissues.
DNA extraction from various mammalian tissues and bacteria in just 8 minutes is followed by PCR amplification by Taq DNA Polymerase 2x Master Mix RED
FEATURES
- Ideal solution for genotyping
- Rapid 8-minute extraction protocol
- Minimal handling
- DNA extracts from mammalian tissues and bacteria
- PCR-ready DNA
- Reliable PCR results
- Direct gel loading
- Scalable set-up
- Automation-friendly
DESCRIPTION
The Q-Extract DNA Extraction PCR Kit consists of Q-Extract DNA extraction solution and Taq DNA Polymerase 2x Master Mix RED.
The non-toxic Q-Extract DNA Extraction Solution is optimized for easy extraction of PCR-ready DNA. DNA can be extracted from various mammalian tissues e.g. mouse tails, ear snips, liver, kidney, lung and saliva. The one-step lysis is performed in ether a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is ready for PCR without further handling such as vortex, centrifugation or dilutions.
The extracted DNA is amplified using the user-friendly Taq DNA Polymerase 2x Master Mix RED for PCR.
Taq DNA Polymerase 2x Master Mix is a ready-to-use reaction solution. The red dye and stabilizer within the master mix formulation enables the user to load amplified PCR samples directly to the DNA gel. The red dye also provides visualization of pipetting and mixing
The obtained DNA extract is stable at – 20°C for up to one week and at – 80°C for long term storage.
The Q-Extract DNA Extraction Solution is also available in a format without Taq DNA Polymerase 2x Master Mix RED.
EFFICIENT AMPLIFICATION OF DNA EXTRACTED FROM VARIOUS MAMMALIAN TISSUES
PERFORMANCE OF Q-EXTRACT DNA EXTRACTION KIT COMPARED TO AN EQUIVALENT PCR KIT FOR GENOTYPING
