Pain and Inflammation

Plantar Test for Thermal Stimulation - Hargreaves Apparatus

Description

Specifications

Documents

Description

Background

Thermal withdrawal latency was first described by Hargreaves et al. (1988; see references)
Compared to the Hot/Cold plate and to Tail Flick tests unilateral/contralateral experiments with internal control was possible and enabled the researcher to discern a peripherally mediated thermal stimulation

Automatic detection for accuracy

The Ugo Basile is the only available system which detects paw withdrawal latency automatically (or optionally manually), thanks to a light detector embedded in the control unit. It comes complete with:

Emitter/Detector unit with strong and focused infra-red light source invisible to the animal
Cross line engraved on the top of the emitter to ease the paw targeting
Touch-screen electronic unit to set all parameters (light intensity, thresholds, etc.) and review data
Data saving in the electronic unit and in the provided USB stick
Data export of the USB stick data into .CSV file for easy analysis in Excel
PC software for animal vivarium management
Platform with semi-transparent glass panel to avoid animal distraction
Animal enclosures for up to 12 mice and 6 rats, fully modular

Features	Benefits
Infrared, strong light source	Invisible to the animal, which is not distracted and high intensity stimulation for short-lasting experiments (a stimulation should not last more than 10-20 seconds, ideally only a few ones)
Embedded light detection	Automated paw withdrawal scoring with no experimenter bias and high accuracy
Optional manual score	The user can decide to manually score the paw withdrawal in those cases where the position of the paw is odd and the automatic detection is not possible
Touch screen control unit	Seamless configuration of the experiment parameters and easy review of data from the control unit
USB stick data export	No need to connect a PC and one-click data export for review in Excel
Modular animal enclosures	The dimension of the area available to the animal can be changed so that it is small but the animal is still unrestrained and amount of habituation can be reduced. High throughput: up to 12 mice and 6 rats

Specifications

General
Command input and readout	via 4.3″ touch-screen
Power requirements	Universal input 100-240 VAC, 50-60Hz, 50W
Data Portability	via USB flash drive provided
Calibration	via appropriate I.R. Radiometer (not included)
Operating Temperature	10° to 40° C, 5% to 95% RH (non-condensing)
Sound Level	<54dB
Operation
I.R. Intensity	Adjustable in the interval 01-99 (in one digit steps)
Latency Time	0.1s steps
Cut off function	From 5 to 30 seconds
Measurement mode	Manual or Automatic
Start experiment	By start icon, push button or TTL input
Stop experiment	By start icon, push button, cut-off, or TTL input
Data export	.csv format, from USB key
TTL I/O	Input and output TTL signal
Physical
Dimensions	135 (w) x 40 (d) x 50 (h) cm (required space on the table)
Weight	11.0Kg
Packing dimensions	98x49x47cm
Shipping Weight	14Kg approx.
Physical animal enclosure setup (internal dimensions)
Mouse	96mm x 96mm x 140mm (h) (max 12 animals)
Rat	96mm x 196 mm x 140mm (h) (max 6 animals)
Fat Rat	196mm x 196 mm x 140mm (h) (max 3 animals)
Warranty
Warranty	37570 is covered by a 12-month warranty + 12 after product registration
UB-Care	Additional UB-Care can be added for other 12 or 24 months

Documents

Dynamic Plantar Aesthesiometer - For Automated Mechanical Stimulation and Allodynia

Description

Specifications

Documents

Description

Background

Von Frey filaments are still a productive way to evaluate behavioral responses of neuropathic pain in preclinical and clinical research.
However, Von Frey tests are time consuming and subject to experimenter bias, and for this reason Ugo Basile developed a fully automated instrument
Moreover, the application of the force is always perpendicular, with a preset amount of grams and at a predefined rate
The Dynamic Plantar Aesthesiometer has been used in a variety of applications in nerve injury, from partial sciatic nerve ligation (PNL, to chronic constriction injury (CCI) and spinal nerve ligation (SNL) to screen phenotypes and drugs to treat allodynia and hyperalgesia

System components and main features

The Ugo Basile system comes complete with:

A movable touch stimulator with standardized filament and angled mirror to facilitate paw targeting
A touch-screen based electronic unit, which controls all the setting parameters, collects data and saves into USB stick for seamless export into CSV, Excel-compatible format
A large testing grid surface with 5×5 mm square holes
A modular set of animal enclosures to hold up to 6 rats and 12 mice

Additional features include:

TTL outputs for start and stop
Automatic or manual detection of paw withdrawal
Analogue output is also available
Filament approaching curve can be adjusted to provide a smoother or lighter first contact with the animal paw, before the force application ramp starts

Feature	Benefit
Fully automated force and force rate application	Higher throughput and improved accuracy
Automated scoring of paw withdrawal	Higher throughput and improved accuracy, thanks to decrease in experimenter bias
Large force range (0,5-100 grams)	Flexibility for performing many types of experiments from severe allodynia to hyperalgesia
Touch force and touch pause can be adjusted	The way the probe approaches the paw can be changed, depending on paw sensitivity
Touch screen and multiple data output (internal storage and CSV export)	Data can be quickly saved in the electronic unit or on the USB stick for CSV export and further analysis
Optional manual scoring of paw withdrawal	Allows for scoring animals which present an unusual position of the paw, and do require the experimenter to visually score
Painted metal grid and modular animal cages	Works with rats and mice in high throughput (6 rats and 12 mice at the time)

Specifications

General
Read out and commands	4,3 inches touch-screen (Resistive)
Power Requirements	Universal input 100-240 VAC, 50-60 Hz, 30W
Sound Level	Negligible
Operating Temperature	10°C to 40°C; 5% to 95% RH (non-condensing)
Operation
Force Range	0.5 to 100 grams, in 0.1g steps
Force Increasing Rate (ramp)	Adjustable in the interval 0 to 50 seconds, in 1 s steps
Latency Time	Display in 0.1s steps
Measurement Mode	Automatic/Manual
Start Experiment	By Start button, push buttons or TTL input
Stop experiment	By Stop button, push buttons, cut-off or TTL input
Data input	Experiment protocol
Data export	.csv format
TTL/IO	Start/Stop, Detection Mode, Force level
Calibration	Calibration Weights (5g, 50g, 100g)
Physical
Total Weight	10Kg
Shipping Weight	13Kg
Dimensions (all parts)	135 (w) x 40 (d) x 50 (h) cm (required space on table; all parts)
Packing Dimensions	98x49x47 cm
Physical animal enclosure setup (internal dimensions)
Mouse	96mm x 96mm x 140mm (h) (max 12 animals)
Rat	96mm x 196 mm x 140mm (h) (max 6 animals)
Fat Rat	196mm x 196 mm x 140mm (h) (max 3 animals)
Warranty
Warranty	Dynamic Plantar Aesthesiometer is covered by a 12-month warranty + 12 after product registration.
UB-Care	Additional UB-Care can be added for other 12 or 24 months

Documents

Electronic Von Frey - e-VF Handheld

Description

Specifications

Documents

Description

Background

Von Frey filaments are still a productive way to evaluate behavioral responses of neuropathic pain in preclinical and clinical research, but they are time consuming and subject to experimenter bias; for this reason Ugo Basile developed a hand held Electronic Von Frey test with automated paw withdrawal detector.
It is important that the application of the force is always perpendicular, with a preset amount of grams and at a predefined rate, which can be controlled with the provided tools (rate-meter on the control unit and slope on the software).
The Electronic Von Frey is based on a force sensor and a peak detection algorithm to automatically score paw withdrawal (a foot pedal for manual score is also included).
One key feature of this device is the possibility to visually control the rate of application of the force by keeping the signal on the slope via software or using the rate-meter on the electronic unit.

System components and main features

The Electronic Von Frey Ugo Basile system comes complete with:

An electronic unit battery powered via USB or the provided charger
A rigid stimulator filament in nitinol (metal)
A prism to aid paw targeting
A pedal for manual scoring
A software for saving force curves, peaks and export data into CSV for Excel
A large testing grid surface with 5×5 mm2 holes (option)
A modular set of animal enclosures to hold up to 6 rats and 12 mice (option)

Features include:

0-50, 0-200, 0-1500 grams ranges to maximize device dynamic range 0.1 gram sensitivity and time resolution of 0.1 seconds
Automatic peak detector and foot pedal
Software included for rate control, waveform saving and CSV data export
Small and handy battery powered electronic unit

Feature	Benefit
Embedded force sensor	No need for multiple filament measurements but just one application of the force
Force Peak detector for automated scoring of paw withdrawal	Higher throughput and improved accuracy, thanks to decrease in experimenter bias
Force Rate Control aids via electronic unit or PC-software	Allows to apply the force at a desidere and adjustable rate
Large force range (0-1500 grams) and high time resolution (0.1 seconds)	Flexibility for performing many types of experiments from severe allodynia to hyperalgesia
Battery powered, small electronic unit with display for settings and results	The system can be easily positioned even away from the mains and can be used with or without software
Grid platform and animal enclosures (optional)	Can be provided as a complete, “ready to go system”. Works with rats and mice in high throughput (6 rats and 12 mice at the time) and it is easy to clean
Optional manual scoring of paw withdrawal	Allows for scoring animals which present an unusual position of the paw, and do require the experimenter to visually score

Specifications

Operation
Commands	Via soft buttons and pedal
Read-out	LCD Screen, via the DCA3 Software on the PC
Start	Automatic start at pressure adjustable threshold
Stop	Automatic at end of pressure, Automatic after a time limit (adjustable from 5 to 25 seconds), Manual by pedal
Force Ranges	o.50, 0-200, 0-1500gf
Force Increasing Rate	Monitored via e-VF Electronic Unit or via the DCA3 Software on the PC
Force Response	in 0.1gf steps
Latency Time	in 0.1s steps
Connection to PC	via USB cable (A to mini-B) and e-VF Software (DCA3)
Power Requirement	Either battery operated, via USB cable connected to PC, included power supply (100-240 VAC, 50-60Hz)
Operating Temperature	10°C to 40°C; 5% to 95% RH (non-condensing)
Sound Level	Negligible

Documents

Incapacitance Tester Librae (Weight Bearing)

Description

Specifications

Documents

Description

Background

Also called “Incapacitance Tester” or “Static Weight Bearing”, it is the classic method to assess unilateral pain (especially joint pain, but also neuropathic pain, etc.), as when the animal stands on the force sensors (one per hind paw), it makes a natural adjustment to the degree of pain, by adapting weight distribution on both rear paws.

Shifts in pain distribution between limbs serve as indicators of pain severity, offering a quantitative and simple method to quantify the extent of weight distribution imbalances caused by pain. Weight bearing devices can also be used in longitudinal studies to understand pain progression and effectiveness of treatments, to screen potential drug candidates as well as in translational studies.

Automated, efficient, accurate

Automation of the measurement process using AutoStart, a unique feature that identifies immobility windows to optimise test repeatability and save operator time.
Option to switch to manual mode with the included foot pedal for hands free operation.
Quick calibration of high quality, precision force sensors (0.1g resolution) using the reference weight supplied.
Maximum weight 500 gr.
Quick, seamless management of settings and data using large, bright, colour touch screen.
Visualization of test data as left/right weight histograms and scatter charts displaying trend data.
Recorded parameters include average paw weight, Standard Deviation, Left/Right ratio and more.
Data is saved in universal .csv format for spreadsheets including Microsoft Excel. Easy transfer of data using supplied USB key.
No screws or protruding parts that can cause interference with the rodents and possibly compromise experiment results.
Fast cleaning of foot pads (within seconds) because they are conveniently attached magnetically for easy removal.

Features	Benefits
Measure hind paw weight distribution	The most widely used method for weight bearing (>1,500 citations) is now updated and equipped with the latest technologies by Ugo Basile.
Autostart is a unique feature	Automate the measurement process by identifying immobility windows.
Touch screen and USB data storage	Let scientists setting, controlling and managing instrument data quickly and seamlessly. Through a few, intuitive buttons, one can reach all the functionalities. The included USB key stores all data for optimal portability (average paw weight, Standard Deviation, Left/Right ratio, etc.). The Left/Right weight histograms and scatter charts display on line the experiment output for an immediate visual control, graphically effective and easy to follow during the test.
Reliable and easy to collect results:, software included	High quality force sensors (0.1g resolution) are easily calibrated with the provided reference weight and show no screws or other parts potentially interfering with the animal, for maximum experiment repeatability. After each test the result can be stored or discarded and data can be transferred from the instrument to a PC for MS Excel. At the end of the experiment the foot pads can be cleaned in a few seconds, being magnetically attached to the base.

Specifications

General
Command input and read-out	4,3 inches touch-screen (Resistive)
Power Requirements	Universal input 100-240 VAC, 50-60Hz, 15W max
Sound Level	Negligible
Operating Environment	10°C to 40°C; 5% to 95% RH (non-condensing)
Operation
Animal weight (per paw)	From 20g to 2200g
Precision	From 0.05% to 0.1%
Measurement time	From 1s to 360s
Measurement starting mode	Manual and Automatic
Calibration	Automatic
Start	Start button, Pedal Switch, or Autostart
Stop	Stop button, Pedal Switch, or Elapsed Timeout
Data Export	.csv format, from USB key (provided)
TTL I/O	Start, Stop, Mode
Physical Librae Incapacitance tester
47880-001	25 cm x 25 cm x 13 cm
Packaging dimension:	45 cm x 34 cm x 26 cm
Weight:	2.5 Kg

Physical Restrainers
Model SKU	Length Internal Base	Length Top	Height	Width	Animal Range
47880-003	34 mm	64 mm	55 mm	40 mm	mouse
47880-002	53 mm	124 mm	89 mm	64 mm	rat up to 200 gr
47880-004	54-78 mm	123-147 mm	89 mm	64 mm	rat up to 250 gr
47880-007	107 mm	189 mm	103 mm	80 mm	rat up to 350 gr
47880-008	117 mm	217 mm	123 mm	100 mm	rat up to 500 gr

Warranty

Warranty

Librae is covered by a 12-month warranty + 12 months after product registration

UB-Care

Additional UB-Care can be added for other 12 or 24 months (see ordering informations)

Documents

Orofacial Stimulation Test - Fehrenbacher, Henry, Hargreaves method

Description

Specifications

Documents

Description

Specifications

General
Water Temperature		Can be adjusted from ambient to 70°C
Mechanical Stimulation		3 mechanical Stimulators with different wire number, and 1 blank
Oro Software		Collects and records beam-break number and duration from up to 16 cages simultaneously
Operating Temperature		10°C to 40°C; 5% to 95% RH (non-condensing)
Sound Level		Negligible
Pollution Degree		≤ 2
Physical
Product Code		Net Weight	Shipping Weight
31300 Net Weight		13Kg	15Kg
31320 Net Weight		23Kg	28Kg
31340 Net Weight		38Kg	43Kg
Warranty
Warranty	Orofacial System is covered by a 12-month warranty + 12 after product registration
UB-Care	Additional UB-Care can be added for other 12 or 24 months

Documents

Thermal Place Preference for Mice and Rats

Description

Specifications

Documents

Description

Background

The hot/cold plate test has some limitations, like the experimenter bias, the lack of consensus around the pain threshold endpoints (first lick latency, frequency of lick, jumping, vocalization), which the Temperature Place Preference overcomes.
In addition to allowing the assessment of temperature preference behaviors, it also automatizes the scoring of the nociceptive behavior if video-tracking is used and makes the scoring more objective anyway, as no action by the operator is required. .

System components and main features

The Ugo Basile Thermal Place Preference includes:
- A Hot/Cold Plate system
- A corridor (choose version for rats or mice)
- A hot plate
- An electronic unit with touch screen to set all parameters and save data
- The Perspex animal restrainers
- USB port to save data and export them in CSV files
- A NEW keypad to score endpoints or any other stereotyped behavior (bluetooth optional)
- A temperature range from -5° to 65° C
- A room ambient temperature sensor
- A very silent operation, even when the peltier elements work at full speed to cool down the device quickly
- A NEW software on the touch screen with several new features, e.g. temperature in C° and °F, an online, an integrated help section, a stop or go option for the end of the ramp mode, a graph showing the plate temperature in real time and a new result screen which is clearer and more comprehensive
- The new software includes all the animal behaviors that can be scored with the 9 keys available
- A PC software to build custom temperature ramps and populate your animal vivarium
Additional features include:
- TTL input/outputs for start/stop and mode command
- USB port for PC connection
- COM port for maintenance purposes
- Ethernet port for support and maintenance (to be used at the factory only)
Optionally, a system with tripod, camera and video-tracking can be purchased to automatize the time spent in each plate, the distance travelled, the number of entries/exits, etc.

Please note: slight variations in the coloration of the plates may occur. This is due to the manufacturing process and does not affect the procedure or the outcome of the experiment in any way.

Features	Benefits
Standard Hot/Cold plate connected to a hot plate	Fixed, ramping and complex ramp temperature modes, for flexibility in performing many types of pain experiments from temperature preference to allodynia and hyperalgesia
Keypad and pedal for scoring pain animal responses and stereotyped behaviours and save the results in the electronic unit	Possibility to use and save the preset 9 keys or modify them (licking, scratching, jumping, grooming, vocalization, rearing, immobility, climbing, shivering, twitching
Broad ramp of temperatures (-5°-65°C)	Allows for any sort of thermal sensitivity experiment, including severe cold allodynia
Ambient temperature sensor is placed outside of the device	Accuracy is much higher
Silent mode of operation or the air dissipation system	Less disturbance to the animal
Control unit and USB key saving	No need to connect a PC
Automatic conversion of data into CSV files for the USB stick	Possibility to open files in Excel
PC software	Possibility to populate your vivarium of animals and to build complex temperature ramps
Optional complete video-tracking software with tripod and camera	Allows for extended and automated data parameters, video saving and no need for the experimenter to be present during the test and to run multiple animals at the same time (with multiple cameras)

Specifications

General
Commands	Hot/Cold Plate: 4”3 touch-screen (resistive), Hot Plate: Themoregulator panel
Read-out	On the touch-screen
Power Requirement	Universal input 85-264 VAC, 50-60Hz, 200W max.
Sound Level	Max 60 dB (A)
Operating Temperature	18° to 25°C
Operation
Temperature	Hot/Cold Plate: adjustable in the range -5.0 to 65.0°C, with 0.1°C increments Hot Plate: from room temperature to 65°C
Precision	+/- 0.1°C
Mode	Hot plate, cold plate, ramp (increasing or decreasing)
Counting Start	By START button or pedal switch
Counting Stop	By pedal switch
Latency Time Counter	Up to 999.9 seconds, in 0.1 steps
Data Acquisition	Via X-PAD software, provided
Data Portability	By USB flash drive, provided
TTL Output	For temperature and counter status
Physical
Total Weight	14Kg
Shipping Weight	20Kg approx.
Dimensions	50(w)x37(d)x47(h)cm – including restrainer
Packing Dimensions	68x34x28cm (two boxes)
Warranty
Warranty	35350 / 35360 are covered by a 12-month warranty + 12 after product registration
Ub-Care	Additional UB-Care can be added for other 12 or 24 months

Documents

Tail Flick Unit - Thermal stimulation, D'Amour & Smith method

Description

Specification

Documents

Description

Background

Performs rapid precise screening of analgesic drugs via heat stimulation according to D’Amour & Smith method.
More than 3,000 citations of our Tail Flick unit in published papers.
Market leading device is the most used and validated worldwide.

Easy use, excellent reproducibility

Works by focusing radiant energy of adjustable intensity on the animal tail (using an embodied parabolic mirror).
Operator holds the animal on a comfortable, flat, unobstructed surface.
Optional inclined mouse restrained is available to ensure flat tail position on surface (to compensate for mouse tendency to hold tail upwards).
When the animal flicks its tail in response to the pain, the sensor detects it, stops the timer and switches off the bulb.
Reaction time (latency) and light intensity data are saved automatically to the unit, USB key (supplied) or “one-touch” export to your PC.
Features a bright graphical touch screen with intuitive controls for easy use.
Easy start, convenient foot pedal switch for excellent reproducibility.
Includes Xpad software.

Feature	Benefits
Tail-flick is scored automatically or manually	Precise score, free of human variability and flexibility
Fully controlled by a 4.3″ touch screen	Ultra-intuitive menus and bright colors for ease of use
USB memory key and software are included	Works as a stand-alone or connected to the PC
No protruding elements and unobstructed surface	Work confortably and get excellent reproducibility
Radiant energy can be calibrated with optional Radiometer	Arbitrary units can be converted and lamp usage compensated

Specification

General
Command input and read-out	4.3″ colour touch screen
Power requirements	Universal input 100-240 VAC, 50-60Hz, 50W
Sound Level	<54db
Operating Environment	10°C to 40°C, 5% to 95% RH (non-condensing)
Operation
Data export	.csv format via USB key
Start Experiment	Via pedal switch or via start icon on the touch screen (or via TTL)
Stop Experiment	Via pedal switch, start icon on the touch screen, cut-off (or via TTL)
Measurement mode	Manual (foot-switch) or automatic (fiber optic)
I.R. Intensity	Adjustable in the interval 01-99 (in one digit steps)
Latency Time	Displayed on the graphic display, in 0.1s steps
Cut-off Function	Adjustable from 5 to 30 seconds
Data Portability	Via USB pen-drive provided
Calibration	Via appropriate I.R. Radiometer (not included)
TTL/IO	Available as input or output
Physical
Total Weight	2.8Kg
Shipping Weight	3Kg approx.
Dimensions	37.5(w) x38.5 (d) x 13 (h) cm (assembled)
Packing Dimensions	46x35x28cm
Warranty
Warranty	Tail Flick Unit is covered by a 12-month warranty + 12 after product registration.
UB-Care	Additional UB-Care can be added for other 12 or 24 months

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Documents

PAM Pressure Application Measurement (for joint pain)

Description

Specifications

Documents

Description

The P.A.M. (Pressure Application Measurement) device is an original tool designed for measuring mechanical pain threshold on joints. It was specifically designed and validated for Arthritis research and is therefore especially suited to assess joint hypersensitivity in rodent knees or ankles. Once saved, data can be browsed on the control unit and/or transferred to a PC in proprietary, Excel or text format, to be managed by most statistical analysis packages available on the market. It can also be used as a Paw Pressure Measurement system with the right adaptor (PN 38500-006).

Feature	Benefits
The force is applied directly to the joint	Direct measurent of evoked pain on the specific site
Pre-calibrated force transducers	No calibration needed
Specifically designed for arthritis research	The applicators are shaped for rodents knee and ankle
Paw pressure transducer as an optional	PAM can also be used as a hand-held Randall-Selitto device
Foot pedal included	Also manual scoring is possible for those cases when the automatic peak detector cannot be used

Specifications

Operation
Commands	Via soft buttons and pedal
Read-out	LCD Screen, via the DCA3 Software on the PC
Start	Automatic start at pressure adjustable threshold
Stop	Automatic at end of pressure, Automatic after a time limit (adjustable from 5 to 25 seconds), Manual by pedal
Force Ranges	0-500gf, 0-1500gf
Force Increasing Rate	Monitored via PAM Electronic Unit or via the DCA3 Software on the PC
Force Response	in 0.1gf steps
Latency Time	in 0.1s steps
Connection to PC	via USB cable (A to mini-B) and PAM Software (DCA3)
Power Requirement	Either battery operated, via USB cable connected to PC, included power supply (100-240 VAC, 50-60Hz)
Operating Temperature	10°C to 40°C; 5% to 95% RH (non-condensing)
Sound Level	Negligible

Physical
Total Weight	0.8Kg
Shipping Weight	2.7Kg approx.
Dimensions	12(w)x15(d)x4,5(h) cm
Packing Dimensions	46x38x27cm
Warranty
Warranty	PAM is covered by a 12-month warranty + 12 after product registration
UB-Care	Additional UB-Care can be added for other 12 or 24 months

Documents

Analgesy-Meter

Description

Specifications

Documents

Description

Measures the force on a rat or mouse paw according to the Randall-Selitto paw-pressure test, it enables rapid and precise screening of analgesic drugs for anti-nociceptive studies, in healthy or inflamed limbs. The device applies a steady increasing force, starting and stopping using the foot pedal. No calibration required. Digital model with force sensors and electronic unit is also available.

Exerts a steadily increasing force with easy start and stop. No calibration required. Design maximises care for animals. Model with digital reading available.

Feature	Benefits
Same instrument, three different force ranges	Applied force: 37215 from 0 to 250, 500, 750grams (with extra weights) 37216 from 0 to 125, 250, 375grams (with extra weights)
No calibration needed	Simple and reliable
Classic method since the 1960s	More than 1000 papers published!
NEW model with digital reading	Upgrade kit for old UB Analgesy-Meters available

Specifications

General
Power Consumption	15W
Operating Temperature	10° to 40° C
Sound Level	Negligible
Operation
Start / Stop	By Pedal Switch
37215 Force Range	From 0 to 250, 500, 750 grams
37216 Force Range	From 0 to 125, 250, 375 grams
Force increasing rate	Regulated by the Analgesy-Meter configuration
Power Requirements	115V 50/60Hz	230V 50/60Hz
Digital data recording	Via optional Analgesy-Meter DAQ 37215-100
Physical
Total Weight	3.3 Kg
Shipping Weight	5.4 Kg approx.
Dimensions	46 x 16 x 14 cm
Packing Dimensions	55 x 45 x 36 cm
Warranty
Warranty	367215 / 37216 are covered by a 12-month warranty + 12 after product registration
UB-Care	Additional UB-Care can be added for other 12 or 24 months

Documents

I.R. Heat-Flux Radiometer

Description

Specifications

Documents

Description

Provides simple, reliable and precise calibration for Ugo Basile Plantar Test & Tail Flick and other infrared (I.R.) analgesia meters.

Feature	Benefits
Calibrate IR emission of Tail Flick and Plantar Test	Ensures that IR emission of different instruments are equal
Provides a measure of stimulus intensity in mW/cm2	Transforms the arbitrary IR intensity in absolute energy values

Specifications

Documents

Von Frey Hairs, Semmes-Weinstein set of monofilaments

Description

Specifications

Documents

Description

Used to measure the paw withdrawal reflex when the mouse or rat paw is unexpectedly touched. For evaluating mechanical allodynia and cutaneous sensation levels. Popular non-invasive technique.

Feature	Benefit
Starts from very low forceas (0.800 gr)	Detects even the most severe allodynia
Retractable filament	Easy to carry and protected from damage
Effective use in clinical setting	Used to diagnose pathologies of hyper- or hypo-aesthesia
Optional perforated platform available	For convenient stimulation of rodent plantar-surface

Set of 20 monofilaments based on the Semmes Weinstein monofilament set. An ascending series of increasingly stiff filaments (from 0.008 to 300 grams). Features retractable head to protect the filaments, allowing a researcher to carry a few around in a pocket. An optional perforated metal grid platform is available that is comfortable for the animal and easy for viewing the target area.

Especially suggested for sub-gram forces (i.e., severe allodynia) although very time consuming and operator dependent. Whenever possible, the use of the Electronic Von Frey or the Dynamic Plantar Aesthesiometer is suggested for higher throughput and better accuracy.

Specifications

Documents

Hot/Cold Plate - Screening of Thermal Hyperalgesia/Allodynia

Description

Specifications

Documents

Description

The hot cold plate test has been the gold standard test for thermal pain tests in rodents for decades. Through the years the devices have been improving and the techniques have become more sophisticated, adding to constant temperature methods also other ones.

The Ugo Basile Hot/Cold plate test includes all the latest technologies necessary to run constant temperature, linear ramping temperature (toward hot or toward cold) and complex temperature ramps thanks to the included X-Pad software.

Moreover, the new model includes a keypad (cabled or optional Bluetooth) to score pain endpoints or stereotyped behaviors.

Temperature range goes from -5° to 65°C and animal holders of different heights are available.

Feature	Benefit
Fixed, ramping and complex ramp temperature modes	Flexibility for performing many types of pain experiments from allodynia to hyperalgesia
Keypad and pedal for scoring pain animal responses and stereotyped behaviors	Possibility to use the preset 10 keys or modify them (licking, scratching, jumping, grooming, vocalization, rearing, immobility, climbing, shivering, twitching) and save the results in the electronic unit
Broad range of temperatures (-5° to -65°)	Allows to perform any sort of thermal sensitivity experiment, including severe cold allodynia
Ambient temperature sensor is placed outside of the device	Improved system and thermal accuracy
Silent mode of operation or the air dissipation system	Less disturbance to the animal
Control unit and USB key saving	No need to connect a PC
Automatic conversion of data into CSV files for the USB stick	Possibility to open files in Excel
PC software	Possibility to populate your vivarium of animals and to build complex temperature ramps

Specifications

General
Commands	4,3 inches touch-screen (Resistive)
Read-out	4,3 inches touch-screen (Resistive), PC
Power Requirement	Universal input 100-240 VAC, 50-60Hz, 200W MAX
Sound Level	70 dB
Operating Temperature	18°C to 25°C
Detection	By pedal switch and via numeric key pad
Operation
Plate temperature range	-5.0°C to +65°C (in 0.1°C steps)
Accuracy	+/- 0.2°C
Mode	Fixed temperature, Ramp (increasing or decreasing), Silent, Fast Ramp, Custom Ramp
Start	By START button or pedal switch
Stop	By STOP button or pedal switch
Experiment duration	Max 999.9 seconds
Data Acquisition	Via X-PAD software (included)
Data Portability	By USB flash drive (included)
Data Input	Personalized protocol
Data Output	Results table in .csv
TTL Output	Temperature, Start, Stop

Documents

Duhram Holders

Description

Specifications

Description

These animal holders complete the scope of the infrared thermal stimulus of the Plantar Test, or the mechanical stimuus of the Dynamic Plantar Aesthesiometer, used for assessing hind paw withdrawal. This new invention allows the application of the same stimulus to the region innervated by the trigeminal nerve.

The Set includes two holders, form molded for testing specific size ranges of animals: the two sizes have been optimized for young adult rats as well as for bigger rats.

Feature	Benefit
Correlation thresholds in submandibular (trigeminal) region and hindpaw plantar surface	A step forward toward multifactorial measurement of pain-related sensitivity in animal research
Test orofacial nociception using a standard Plantar thermal or mechanical stimulator	The new holders complete the scope of the thermal or mechanical stimulators used for assessing hind paw withdrawal

Specifications

Physical
Material	Deep-Red Color Polymer
Weight	0.4 Kg
Shipping Weight	1.0 Kg
Packing dimension	39 x 27 x 21 cm
Warranty
Warranty	Durham Holder is covered by a 12-month warranty + 12 after product registration
UB-Care	Additional UB-Care can be added for other 12 or 24 month

Thermal Gradient Ring (TGR) 2.0

Description

Specifications

Description

The TGR can clearly discriminates temperature-dependent phenotypes or temperature dependent drug effects.

It records and analyses thermal preference/avoidance in mice. For neuropathic pain studies, peripheral neuropathy, temperature sensitivity and insensitivity assessment in basic research, phenotyping and drug screening.

The animal is freely moving, its position is tracked by a camera, and no user intervention is required to gather time spent in each temperature zone (12 zones in duplicate) and many other parameters, like the preferred temperature zone.

In the TGR mice can freely move around the ring floor, thereby avoiding the stereotypical habit that mice have of staying in a corner, as occurs in rectangular systems.

It is a cost effective and bias-free tool to obtain reproducible data in a very broad range of thermal stimulation experiments.

The new version is designed to use also cabled animals for optogenics, electrophysiology and other techniques which require the use of wires.

Features	Benefits
New circular design, internal diameter 45cm, outer diameter 57cm	Duplicate values, no border effects, no spatial cues
New design which includes swivel support and has no obstruction for animal mounted wires	Allows for utilization of techniques that required cabled animals, such as optogenetics, electrophysiology and others
Thermal Insulated Ring-shaped Aluminum Runway	More sensitive than previous methods: bias-free, reproducible data
Heater and cooler on opposite sides, to establish a symmetric gradient	Gradient setup superior to two-plate choice design
12 zones per side (specular), 40cm² each	Temperature Δ proportionally divided into 12 (in the method paper 15°C-40°C = 2.27°C per zone)
4 thermocouples embedded in the thermal gradient ring	The exact temperature gradient measured in real time
CCD-camera (included in the standard package, with its dedicated support) and ANYmaze video-tracking software	Behavior recorded automatically during 60 minutes
The system includes a set of 4 dual (visible/I.R.) lights	Ideal for videotracking: perfect view even in the dark
Control ambient temperature	Improve temperature accuracy avoiding laboratory temperature oscillations

Specifications

General
Controls	Commands on the heater/cooler front panel
Preset Temperature Read-out	On LCD display on the front panel of the thermal units
Detection	Via ANY-Maze specific protocol for TGR through integrated USB camera
Sound emission	68 dB
Power requirements	Universal input 85-264 VAC, 50-60Hz, 400W max
Communication interface	Integrated 4 ports USB hub
Operation
Temperature Range	Heating Plate: from 20°C to 65°C Heating/cooling Plate: from 4°C to 65°C
Precision	1°C
Temperature feedback	By 4 thermocouples monitored in real time by ANYmaze
Light Intensity (I.R.)	Set by potentiometer with graded scale
Light Intensity (visible)	Set by potentiometer with graded scale
Physical
Internal diameter	45 cm
Outer diameter	57,5 cm
Corridor Width	6 cm
Wall Height standard	15 cm
Wall Height optional	25 cm
Dimensions	76x60x60(h)cm
Weight	49.5 Kg
Shipping Weight	69.5 Kg
Packing Dimensions	120x80x70(h)cm (wooden fireproof crate with pallet)
Warranty
Warranty	35550 is covered by a 12-months warranty + 12 months after product registration
UB-Care	Additional UB-Care can be added for other 12 or 24 months

Plethysmometer - Paw Volume & Oedema

Description

Specifications

Documents

Description

Measures small changes in volume & oedema (fluid retention) to gauge the inflammatory response such as for anti-inflammatory screening tests.

Hands-free operation. Precision measurements. Detects and displays 0.01 ml changes in rodent paw volume. Easy data transfer and software included.

Feature	Benefits
Touch screen display	Easy to operate, easily result data viewing
Auto-measure function	Easy operation and better measuring accuracy and repeatability
USB pen drive	For fast measured data retrieving and experiment data loading
Web based LAN connection	Remote data retrieving and experiment data loading
Foot-Pedal to freeze the reading	Hand-free operation
More than 1000 citations since 1960s	Most applications can be found in bibliography for reference
Interchangeable water cells	Same instrument for mice and rats (four different dimensions)

Specifications

Operation
Power Requirement	Universal input 85-264 VAC, 50-60Hz, 15 W max
Data Read–out	Multifunction graphic display
Display	4.3” multifunction color touch-screen display
Data Format	4 digits 2 integers, 2 decimals (e.g.:23.89 ml)
Physical
Weight	3,5 Kg
Shipping Weight	6.5 Kg
Shipping Dimension	36x55x45 cm
Warranty
Warranty	Plethysmometer is covered by a 12-month warranty + 12 after product registration
UB Care	Additional UB-Care can be added for other 12 or 24 months

Documents

Climbing Test - Measures Vertical Activity in Rodents

Description

Specifications

Documents

Description

Background

The device, developed in collaboration with the laboratory of Prof. Sidney Negus from Virginia Commonwealth University, provides a measurement of the vertical movement of rodents, a parameter which has not been widely investigated so far, in spite of the fact that rodents are animals that live in a 3D space and hence the analysis of XY position only is a limitation in many current studies.

However, climbing behavior and vertical movements have been previously studied in non-automated experiments to investigate many fields, from muscle strength (Ueno et al. 2022), to antidepressants (Perona et al. 2008), stroke (Jin et al., 2017), Parkinson’s (Sundstrom et al., 1990), sex-differences (Borbelyova et al., 2019), locomotor activity and kinematics (Green et al., 2012), neuroleptics and dopamine agonists (Costall et al., 1982; Pinsky et al., 1988; Medvedev et al., 2013), XYZ activity (Wexler et al. 2018), opioid receptors (Michael-Titus et al., 1989), neuropathic and cancer pain (Falk et al., 2017).

The Ugo Basile Climbing System aims to provide a simple and automated tool to measure vertical climbing movements within a grid cylinder to inform all the scientists that are interested in this crucial parameter for their research.

Climbing Test System Components

A load cell underneath the aluminum base (diameter 12 cm) provides the automated start of the test once the animal leaves the base and starts climbing over the cylinder (height 25.5 cm) and its ceiling, thanks to the 5x5mm internal grid.

The output parameters, stored in the electronic unit and exportable in Excel, include the %climbing, the number and duration of climbing episodes, the climbing time, the maximum and average climbing height and the total activity while climbing.

Features	Benefits
Conductive sensors to track Z movements	Automated measurement of time climbing, distance travelled, time on ceiling, climbing episodes
Load cell floor	Automated start of the test
Electronic unit	Controlled by touch screen and with internal memory
USB and LAN data saving	Easy export into excel and data security

Specifications

General
Commands	4,3 inches touch-screen (Resistive)
Read-out	4,3 inches touch-screen (Resistive), PC
Power Requirement	Universal input 100-240 VAC, 50-60Hz, 200W MAX
Sound Level	Negligible
Operating Temperature	18°C to 25°C
Endpoints and statistics:	Max height, Average height, Average distance covered, Total climbing time, Total time on roof, Latency on the single climbing event
Operation
Start/Stop	By Start/Stop button on the climbing cage
Tare	By Tare button on the climbing cage
Experiment duration	Max 3600 seconds
Height sensors	7 rings + 1 on the roof (capacitive sensors)
Data Acquisition	Via X-PAD software (included)
Data Portability	By USB flash drive (included) or LAN connection
Data Output	Results table in .csv
Physical
Sensing cylinder diameter	External diameter 12 cm. Internal Diameter 11,5 cm
Grid cylinder diameter	11 cm
Sensing cylinder height	29 cm
Grid cylinder height	28 cm
Weight	5Kg
Shipping Weight	8Kg
Packing Dimensions	36x55x45cm
Warranty
Warranty	36103 is covered by a 12-months warranty + 12 months after product registration

Documents

Pain and Inflammation

Plantar Test for Thermal Stimulation - Hargreaves Apparatus

Background

Automatic detection for accuracy

Dynamic Plantar Aesthesiometer - For Automated Mechanical Stimulation and Allodynia

Background

System components and main features

Electronic Von Frey - e-VF Handheld

Background

System components and main features

Incapacitance Tester Librae (Weight Bearing)

Background

Automated, efficient, accurate

Orofacial Stimulation Test - Fehrenbacher, Henry, Hargreaves method

Background

High throughput, simultaneous measurement

Thermal Place Preference for Mice and Rats

Background

System components and main features

Tail Flick Unit - Thermal stimulation, D'Amour & Smith method

Background

Easy use, excellent reproducibility

PAM Pressure Application Measurement (for joint pain)

Analgesy-Meter

I.R. Heat-Flux Radiometer

Von Frey Hairs, Semmes-Weinstein set of monofilaments

Hot/Cold Plate - Screening of Thermal Hyperalgesia/Allodynia

Duhram Holders

Thermal Gradient Ring (TGR) 2.0

Plethysmometer - Paw Volume & Oedema

Climbing Test - Measures Vertical Activity in Rodents

Background

Climbing Test System Components

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