Nano Spectrophotometers and Fluorometers
Smart-Q Fluorometer
Smart-Q 100 for single sample processing
Smart-Q 800 for up to 8-sample processing
Accuris DNA Quantification Kits
The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving:
- Next Generation Sequencing
- PCR
- Transfection
- Western blotting
- Immunoassays
The Smart-Q Fluorometers are engineered to achieve optimal performance with Accuris’ Fluorescent DNA Quantification Kits and assay tubes; however, they also maintain compatibility with a range of kits offered by various other suppliers.
Intuitive Usability
Each instrument is equipped with a 7-inch color touchscreen, featuring an intuitive, icon-driven user interface. Preprogrammed protocols allow for quick & effortless assay set-up. Results are quickly calculated and displayed in less than 6 seconds, and saved to the instrument’s internal memory. All data can also be exported easily to a PC using a USB flash drive.
Accurate Quantification
The Smart-Q Fluorometers, along with their accompanying assay kits, are engineered for the precise measurement of fluorescent dye signals that are bound to targeted biological molecules. These specially formulated dyes exhibit selective affinity towards DNA, RNA, or proteins, and generate fluorescent signals exclusively upon binding to their respective targets. Both Smart-Q models include Blue and Red channels that allow for various fluorescent dyes to be used in nucleic acid and protein measurements.
The Smart-Q Fluorometers incorporate advanced curve-fitting algorithms to establish calibration curves from standard samples of known concentrations.
The concentration of an unknown sample, whether it be DNA, RNA, or protein, is determined by correlating its Relative Fluorescence Units (RFUs) with those of the standards employed during calibration. The measurement sensitivity for each type of assay is distinct and defined by its detection limits.
Options for Throughput
The Smart-Q portfolio includes two distinct models, each designed to meet specific laboratory requirements:
The Smart-Q 100 allows for single-sample processing in 0.5mL assay tubes (NS1020-T). This renders the Smart-Q 100 ideal for laboratories processing fewer samples.
The Smart-Q 800 caters to high-throughput workflows and allows for up to eight samples to be evaluated at once using individual 0.2mL tubes or 8 x 0.2mL strips tubes (Order no. F1001-T and F1001-T-8, ). The use of a standard 8-channel pipettor can streamline both the reagent preparation and sample transfer, making the Smart-Q 800 an efficient solution for workflows requiring parallel processing of multiple samples.
Accuris DNA Quantification Kits
Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using an instrument such as the SmartDrop XF, Qubit™or Accuris™ Fluorometer. The reagents are highly selective for double-stranded DNA (dsDNA), and not for single-stranded DNA (ssDNA), RNA, protein, or nucleotides. Included with each kit are a concentrated assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, sample is added (1 µL to 20 µL of sample is needed), and the concentration can be determined using an appropriate fluorometer instrument.
High Sensitivity dsDNA Assay Kit
The Accuris High Sensitivity kit provides an accurate method for quantitation of dsDNA samples when sensitivity is required. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.005 to 120 ng/µL, providing a detection range of 0.1−120 ng. 0.1 to 100 ng/ul, providing detection of 0.1 to 100 ng
Broad Range dsDNA Assay Kit
The Accuris Broad Range kit provides an accurate method for quantitation of dsDNA samples for a broad range quantity of DNA. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng.
| F1001 | Smart-Q 100 Fluorometer, single sample, 115V |
| F1001-E | Smart-Q 100 Fluorometer, single sample, 230V |
| F1008 | Smart-Q 800 Fluorometer, 8-channel, 115V |
| F1008-E | Smart-Q 800 Fluorometer, 8-channel, 230V |
| F1000-HS-100 | High Sensitivity dsDNA Quantification Kit, 100 assays |
| F1000-BR-100 | Broad Range dsDNA Quantification Kit, 100 assays |
| F1001-T | 0.5mL Fluorescence Detection Tubes, pack of 250 |
| F1008-T | 0.2ml Smart-Q™ 800 Assay Tubes, pack of 1000 |
| F1008-T-8 | 0.2ml Smart-Q™ 800 Assay Tube Strips, pack of 120 |
Smart-Q Fluorometer, for quantification of DNA, RNA and protein samples
Sensitive quantitation down to 0.1pg/ul ds DNA
Large color touch screen for programming and operation
Two models for single or 8-sample processing
Built-in reagent calculators for sample preparation
Compact and portable (11 x 6 inch footprint)
| Model | Smart-Q™ 100 Fluorometer | Smart-Q™ 800 Fluorometer |
|---|---|---|
| Display | 7.0-inch, color touch screen | 7.0-inch, color touch screen |
| Dynamic Range | 5 orders of magnitude | 4 orders of magnitude |
| Light Source | Blue and Red LED | Blue and Red LED |
| Excitation Wavelengths | Blue: 460 ± 20nm and Red: 625 ± 20nm | Blue: 460 ± 20nm and Red: 625 ± 20nm |
| Emission Wavelengths | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm |
| Stability | ≤ 1.5% | ≤ 1.5% |
| Detector | Photodiodes (300-1000nm) | Photodiodes (300-1000nm) |
| Measurement Speed | ≤ 5 seconds | ≤ 6 seconds |
| Consumable type | 0.5mL assay tubes | 0.2mL assay tubes (single and 8-tube strip) |
| Input Voltage | 24 VDC 2A | 24 VDC 2A |
| Dimensions (WxDxH) | 16.1 x 28.7 x 6.1cm / 6.34 x 11.30 x 2.40in. | 16.1 x 28.7 x 7.6 cm / 6.3 x 11.3 x 3.0 in. |
| Weight | 1.6kg / 3.5lbs | 2.0kg / 4.4lbs |
SmartDrop Nano Spectrophotometers
The Accuris SmartDrop™ series of nano spectrophotometers provides quick and easy quantification of small samples. Utilizing a small sample volume eliminates the need for dilutions, providing more accurate results.
A full color, 7” touchscreen with icon-driven menus and preprogrammed protocols gets users up and running quickly. No external computer is required. Each unit features a quartz pedestal onto which a drop of sample is placed. The arm is lowered and the drop is stretched into a liquid column of specificed length for spectral analysis. DNA, RNA and protein concentrations and purity numbers are displayed in a matter of seconds.
The resulting data can be printed, saved in the SmartDrop’s onboard memory or exported via USB as an Excel file. All SmartDrop models include an integrated printer. For OD600 readings, each model includes a cuvette port and two optical glass cuvettes. Three SmartDrop models are available to meet a range of applications and budgets.
SmartDrop XF with Fluorometer
NS1020-T, 0.5mL Fluorescence Detection Tubes, pack of 250
NS1020-HS-100, NS1020-BR-100
DNA Quantification Kits
SmartDrop L
- Ideal for basic DNA/RNA/protein quantification and purity
- Affordable LED and silicon detector technology
- Fixed 0.5mm path length, detect up to 2500ng/µl of dsDNA
SmartDrop L is the affordable choice for laboratories needing to determine nucleic acid and protein concentrations and purity of samples. The LED light source and silicon photocell sensor provide readings at 260 and 280nm. Samples from 1.0 to 2.0µl are loaded directly on the pedestal. Results are provided in about 5 seconds. The software automatically
calculates and displays 260/280 ratios.
SmartDrop X
- Full-spectrum absorbance scans from 200 to 800nm
- Quickly calculates and displays 260/280 and 260/230 ratios
- Long-life Xenon lamp with precision monochromator
- Auto-ranging path lengths of 0.2mm and 1.0mm
- Detect up to 4,500 ng/µl of dsDNA without dilution
A full spectrum UV-Vis spectrophotometer, SmartDrop X requires 0.5 to 2.0µl of sample. In addition to nucleic acid and protein calculations, the software has preset programs for BCA, Bradford and Lowry assays. A full spectrum analysis can be performed in about 6 seconds, aiding in the
identification of unknowns. Up to 5 specific points on the spectrum can be selected for colorimetric readings output. The long life Xenon lamp source requires no warm up and is paired with a highly sensitive CCD detector. To provide the most accurate results, the SmartDrop X utilizes 2 path lengths.
SmartDrop XF – Spectrophotometer + Fluorometer
- Full-spectrum absorbance scanning and calculations of
260/280 and 260/230 ratios - Long-life Xenon lamp with precision monochromator
- Auto-ranging path lengths of 0.05mm, 0.2mm and
1.0mm - Detect up to 15,000ng/µl of dsDNA without dilution
- Integrated fluorometer for sensitive quantitation of low
concentration nucleic acids
This top of the line spectrophotometer can perform not only UV-Vis readings in the range of 200-800nm but also fluorescence readings. Like the SmartDrop X, the SmartDrop XF features predefined programs for nucleic acids, proteins, and various colorimetric assays. The patented, motorized system gently stretches the sample column and allows for three different path lengths, providing accurate readings of samples from 0.5 to 2.0µl without dilution.
The integrated fluorometer yields more accurate, sensitive and selective quantitation of nucleic acids. The blue light channel (460nm) is useful for detection of dyes such as PicoGreen and RiboGreen as well as GFP and fluorescein. UV, green and red channel models are available upon
request.
Accuris DNA Quantification Kits
Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using instruments such as the Qubit®, SmartDrop™ XF, and microplate readers. The reagents are highly selective for double-stranded DNA (dsDNA); quantification results will not be skewed by single-stranded DNA (ssDNA), RNA, protein, or nucleotides in a sample. Included with each kit are a concentrated fluorescent assay reagent, dilution buffer, and ready-to-use DNA standards. The assay reagent is diluted using the buffer provided, sample is added (1 µL to 20 µL), and the concentration determined using an appropriate fluorometer instrument that utilizes blue excitation (460 ± 20 nm) and measures green fluorescence (525-570 nm).
High Sensitivity dsDNA Assay Kit
The Accuris High Sensitivity Kit provides an accurate method to quantify low concentration dsDNA samples. The assay kit is designed to be accurate for initial DNA sample concentrations of 0.005–120 ng/µl, providing a detection range of 0.1–120 ng.
Broad Range dsDNA Assay Kit
The Accuris Broad Range Kit provides an accurate method to quantify dsDNA samples that have a broad concentration range starting at 0.2 ng/µL. The assay kit is designed to be accurate for initial DNA sample concentrations of 0.2 to 2,000 ng/µL, providing a detection range of 4−2,000 ng
SmartDrop Nano Spectrophotometers for Microvolume Analysis of DNA, RNA and Proteins
Quick and accurate results from a sample droplet
Touch screen control panel, no PC or software required
Built-in printer and cuvette port for OD600 readings
XF model includes fluorometer for sensitive DNA
quantification
| Model | SmartDrop L (NS1000) | SmartDrop X (NS1010) | SmartDrop XF (NS1020) |
| Display | 7-inch touch screen | 7-inch touch screen | 7-inch touch screen |
| Minimum sample size | 1.0µl – 2.0µl | 0.5µl – 2.0µl | 0.5µl – 2.0µl |
| Path length | 0.5mm | 0.2mm, 1.0mm | 0.05mm, 0.2mm, 1.0mm |
| Light source | UV LED | Xenon Flash Lamp | Xenon Flash Lamp |
| Detector | UV Silicon Photocell | 2048 Element Linear Silicon CCD Array | 2048 Element Linear Silicon CCD Array |
| Wavelength | 260, 280, & 600nm | 200 – 800nm (1nm steps) | 200 – 800nm (1nm steps) |
| Absorbance range | 0.2 – 50 A (10mm equivalent) | 0.04 – 90 A (10mm equivalent) | 0.04 – 300 A (10mm equivalent) |
| Absorbance precision | 0.005 A | 0.003 A | 0.003 A |
| Lower limit of detection | dsDNA: 10ng/µl; BSA: 0.1 mg/ml | dsDNA: 2ng/µl; BSA: 0.1mg/ml | dsDNA: 2ng/µl ; BSA: 0.1mg/ml |
| Upper limit of detection | dsDNA: 2,500ng/µl; BSA: 75.0 mg/ml | dsDNA: 4,500ng/µl; BSA: 135mg/ml | dsDNA: 15,000ng/µl; BSA: 360mg/ml |
| Detection time | < 6 seconds | < 5 seconds | < 6 seconds |
| Communication ports | USB for data export | USB for data export | USB for data export |
| Voltage input | DC 24V, 2A | DC 24V, 2A | DC 24V, 2A |
| Power | 25W | 25W | 25W |
| Dimensions (WxDxH) | 8.2 x 11.0 x 7.3 in. / 20.8 x 28.0 x 18.6cm | 8.3 x 10.6 x 7.2 in. / 21.0 x 26.8 x 18.1cm | 8.2 x 12.6 x 7.3 in. / 20.8 x 32.0 x 18.6cm |
| Weight | 3.6 kg / 7.9 lbs | 2.8 kg / 6.2 lbs | 3.6 kg / 7.9 lbs |
| Cuvette OD600 detection | |||
| Absorbance range | 0.0 – 4.0 A | 0.0 – 4.0 A | 0.0 – 4.0 A |
| Absorbance stability | [0,3) ≤ 0.5%, [3,4) ≤ 2% | [0,3) ≤ 0.5%, [3,4) ≤ 2% | [0,3) ≤ 0.5%, [3,4) ≤ 2% |
| Absorbance repeatibility | [0,3) ≤ 0.5%, [3,4) ≤ 2% | [0,3) ≤ 0.5%, [3,4) ≤ 2% | [0,3) ≤ 0.5%, [3,4) ≤ 2% |
| Absorbance precision | [0,2) ≤ 0.005 A, [2,3) ≤ 1%, [3,4) ≤ 2% | [0,2) ≤ 0.005 A, [2,3) ≤ 1%, [3,4) ≤ 2% | [0,2) ≤ 0.005 A, [2,3) ≤ 1%, [3,4) ≤ 2% |
| Fluorescence detection | |||
| Excitation/Emission | N/A | N/A | 460 ± 20nm / 525-570nm |
| Sensitivity | N/A | N/A | dsDNA: 0.5pg/µl |
| Linearity | N/A | N/A | R² ≥ 0.995 |
| Repeatability | N/A | N/A | ≤ 1.5% |
| Stability | N/A | N/A | ≤ 1.5% |
