End Point PCR Reagents
- End Point PCR Reagents
- qPCR and cDNA Synthesis Kits
- Laboratory Weighing
- Accuris Liquid Handling
- Electro Phoresis
- Microplate - Instruments
- Automated Cell Counter
- Magnetic Bead Based Purification
- Nano Spectrophotometers and Fluorometers
- Benchmark Scientific Products
Fast Extraction PCR Kit
The Accuris Fast Extraction PCR Kit features an exclusive single-tube solution that extracts genomic DNA in just 8 minutes. This is swiftly followed by PCR amplification utilizing our Hot Start Red Dye Master Mix, designed for superior endpoint PCR performance. Hot Start Red Dye Master Mix is a 2X formula that incorporates a non-reactive red dye, enabling direct gelloading.
This kit offers versatility to suit different workflows: the PCR prepared genomic DNA can be directly used in endpoint PCR, or can be applied in real-time PCR using either SYBR Green or TaqMan Probe chemistries.
Each 100 reaction kit includes:
- 1 tube of High Fidelity Hot Start Red Dye Master Mix (1.25mL)
- 1 tube of Fast Extraction Lysis Solution (1.25mL)
Applications: Extraction & Amplification of Tissue, Plant, Saliva, &
Bacteria
The Accuris Fast Extraction PCR Kit offers exceptional versatility and can be used to extract and amplify DNA from a wide range of sample types
• Compatible with animal tissue, plant, saliva, & bacterial samples
• Extraction & Amplification in less than 60 minutes
• Includes High Fidelity Hot Start Red Dye Master Mix to prevent the risk of nonspecific amplification & to visualize bands easily
• Process extracted sample via PCR with included mastermix
• Alternatively amplify via qPCR (qPCR mastermix not included
| PR1400-FE-S | Accuris Fast Extraction PCR Kit Sample 16 reactions |
| PR1400-FE-100 | Accuris Fast Extraction PCR Kit 100 reactions |
| PR1400-FE-400 | Accuris Fast Extraction PCR Kit 400 reactions |
| PR1400-FLS-S | Accuris Fast Extraction Lysis Solution 16 reactions |
| PR1400-FLS-100 | Accuris Fast Extraction Lysis Solution 100 reactions |
| PR1400-FLS-400 | Accuris Fast Extraction Lysis Solution 400 reactions |
One-Step RT-PCR
The Accuris One-Step RT-PCR Kit has been formulated for cDNA synthesis and subsequent PCR in a single tube for end-point analysis. This latest generation RT-PCR Kit consists of an MMLV-derived, thermostable Reverse Transcriptase (45°C to 55°C), an advanced RNase Inhibitor and Accuris Hot Start Taq for ultra-sensitive one-step RT-PCR from as little as 1pg total RNA starting material.
The optimized buffer chemistry allows for efficient reverse transcription and PCR of problematic sequences with significant secondary structure (such as GC-rich targets). The Accuris One-Step RT-PCR Kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative analysis of RNA transcription levels. The kit also efficiently synthesizes double-stranded cDNA for subsequent gene expression analysis.
Convenient cDNA synthesis and PCR in a single tube from 1pg total RNA
Formulated for highly specific and sensitive RT-PCR from any RNA templates
Incorporates thermostable reverse transcriptase and Hot-Start Taq for preparation at room-temperature
| Item No. | Description |
|---|---|
| PR1100-50 | Accuris One-Step RT-PCR kit, 50 reactions |
| PR1100-100 | Accuris One-Step RT-PCR kit, 100 reactions |
Accuris dNTPs
The success of your PCR reaction depends on the quality of the starting materials. Accuris dNTP’s are purified by HPLC in a strict process that results in greater than 99% purity. The stringent purification process eliminates PCR inhibitors such as tetraphosphates and pyrophosphates that can interfere with the sensitivity of your PCR and reduce yields.
Extensive quality control testing is performed to ensure the dNTPs are free of nuclease, protease and nickase activity. Each lot is performance tested in standard PCR, long PCR and qPCR reactions to assess reproducibility and sensitivity.
The 40mM dNTP Mix is a single tube that contains premixed dNTPs at a concentration of 10mM each. The 100mM dNTP Set contains four individual tubes, one each of dATP, dCTP, dGTP and dTTP. The nucleotides are supplied in ultra-pure water as an ammonium salt. Both the set and mix are stable for 24 months when stored at -20ºC.
- Supplied as a ready-to-use 40mM mix or a set of 4 separate 100mM solutions
- Free of impurities and inhibitors that reduce sensitivity and yield
- No nuclease, protease or nickase activity
- >99% pure, purified by HPLC
- 24 month shelf life
| PR3040-M-1 | 40mM dNTP Mix, ready-to-use, 10mM/20μmol each, 0.5ml |
| PR3040-M-4 | 40mM dNTP Mix, ready-to-use, 10mM/80μmol each, 2ml |
| PR3101-S-1 | 100mM dNTP Set, dATP, dCTP, dGTP, dTTP, 250μl/25μmol each |
| PR3101-S-4 | 100mM dNTP Set, dATP, dCTP, dGTP, dTTP, 1ml/100μmol each |
Accuris Taq
Accuris Taq provides superior results for routine applications. Modified to improve DNA-binding, this polymerase offers higher solubility and greater template affinity, resulting in consistently superior performance.
- Ideal for routine PCR applications as well as genotyping, colony PCR and fast PCR.
- Improved template affinity and solubility for higher enzyme activity and greater yields.
- Proprietary buffer optimized for a variety of assay conditions.
- Conveniently supplied as a 2X Master Mix or in a 2-tube format of polymerase and separate 5X buffer.
| Item No. | Description | Size |
|---|---|---|
| PR1000-500 | AccurisTaq DNA Polymerase 500u (5u/µl) | 500u (5u/µl) |
| PR1000-1000 | Accuris Taq DNA Polymerase 1000u (5u/µl) | 1000u (5u/µl) |
| PR1000-6000 | Accuris Taq DNA Polymerase 6000u (5u/µl) | 6000u (5u/µl) |
| PR1001-200 | Accuris 2xTaq Master Mix 200 x50µl Reactions | 200 x50µl Reactions |
| PR1001-1000 | Accuris 2x Taq Master Mix 1000 x 50µl Reactions | 1000 x 50µl Reactions |
| PR1001-R-200 | Accuris 2x Taq Red Dye Master Mix 200 x 50µl Reactions | 200 x 50µl Reactions |
| PR1001-R-1000 | Accuris 2x Taq Red Dye Master Mix 1000 x 50µl Reactions | 1000 x 50µl Reactions |
Accuris Hot Start Taq
Engineered for controlled polymerase activity, Accuris Hot Start Taq is bound with a monoclonal antibody that blocks enzyme activity. This allows reactions to be set up at room temperature without the risk of non-specific amplification.
- Exceptional sensitivity for low copy PCR.
- Ideal for multiplex PCR and amplification of GC-rich DNA.
- Same enhanced features as Accuris Taq Polymerase.
- Buffer optimized for fast cycling and reproducibility.
| Item No. | Description | Volume |
|---|---|---|
| PR1000-HS-500 | Accuris Hot Start Taq DNA Polymerase | 500u (5u/µl) |
| PR1000-HS-1000 | Accuris Hot Start Taq DNA Polymerase | 1000u (5u/µl) |
| PR1000-HS-6000 | Accuris Hot Start Taq DNA Polymerase | 6000u (5u/µl) |
| PR1001-HS-200 | Accuris Hot Start 2X Taq Master Mix | 200 x 50µl Reactions |
| PR1001-HS-1000 | Accuris Hot Start 2X Taq Master Mix | 1000 x 50µl Reactions |
| PR1001-HSR-200 | Accuris Hot Start 2X Taq Red Master Mix | 200 x 50µl Reactions |
| PR1001-HSR-1000 | Accuris Hot Start 2X Taq Red Master Mix | 1000 x 50µl Reactions |
Accuris High Fidelty
Accuris High Fidelty Polymerase (2 tube formulation)
- Leaves a blunt end for blunt end vector cloning
- 50x fidelity of native Taq
- Ideal for complex targets up to 10kb
For applications requiring highly accurate amplification, and full control of enzyme concentration, choose our High Fidelity DNA polymerase kit. Our polymerase enzyme has been genetically modified for better solubility and higher activity across a broad range of ionic conditions, and will amplify a wide range of targets, including those that are GC or AT rich as well as crude DNA samples.
A 3′-5′ proofreading exonuclease activity and an error rate of 4.55×10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The included 5X buffer contains an optimized concentration of dNTPs and enhancers for compatibility with a variety of amplicons up to 10kb in length.
Accuris High Fidelity Master Mix
- Leaves a blunt end for blunt end vector cloning
- 100x fidelity of native Taq
- Ideal for shorter, less complex targets
A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, Accuris High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.
The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.
Accuris High Fidelity, Hot Start Master Mix
- Leaves an A-overhang for TA cloning
- 10x fidelity of native Taq
- Ideal for difficult, high GC content sequences up to 10kb
Accuris High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, and an antibody mediated hot-start polymerase with proof-reading component for trouble-free PCR reaction assembly and performance.
The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.
For applications requiring highly accurate amplification, Accuris offers a range of high fidelity PCR options. Our proofreading enzymes are modified for better sensitivity and higher activity, and this ensures more reproducible performance with a wide range of targets.
- Up to 100X higher fidelity than Taq polymerase.
- Crude DNA sample will not inhibit amplification.
- Ideal for cloning, mutagenesis, sequencing and microarrays.
- Options for blunt end or A-overhang products.
- Optimized buffers include PCR enhancers.
| Item No. | Description | Volume |
|---|---|---|
| PR1000-HF-200 | Accuris High Fidelity DNA Polymerase | 200u (2u/µl) |
| PR1000-HF-1000 | Accuris High Fidelity DNA Polymerase | 1000u (2u/µl) |
| PR1001-HF-200 | Accuris High Fidelity Master Mix | 200 x 25ul rxns |
| PR1001-HF-500 | Accuris High Fidelity Master Mix | 500 x 25ul rxns |
| PR1001-HFHS-200 | Accuris High Fidelity, Hot Start Master Mix | 200 x 25ul rxns |
| PR1001-HFHS-500 | Accuris High Fidelity, Hot Start Master Mix | 500 x 25ul rxns |
Mammalian Genotyping
The Accuris 1 Hour Mammalian Genotyping Kit is quick and easy – add sample to the proprietary buffer and incubate for 5-10 minutes, then add the deactivation buffer and incubate for 10 minutes.
- DNA extraction and amplification in 1 hour.
- Proprietary lysis buffer optimized for ear punches, tail snips and other mammalian tissues.
- Single tube – no organic solvents or clean up procedures.
- Enhanced Accuris Hot Start Polymerase included.
| Item No. | Description | Reactions |
|---|---|---|
| PR1300-MG-80 | Accuris 1 Hour Mammalian Genotyping kit | 80 |
| PR1300-MG-400 | Accuris 1 Hour Mammalian Genotyping kit | 400 |
