Indicator Organisms
MicroSnap Coliform
MicroSnap Coliform is a rapid bioluminogenic test method for the detection and enumeration of coliform bacteria, giving actionable same-day test results. The MicroSnap™ test platform consists of an Enrichment Device containing a specific growth medium and a Detection Device containing a bioluminogenic (light-producing) substrate. When MicroSnap™ Coliform detects the specific microorganism, light is emitted and measured with a Hygiena™ luminometer. Results are stored and tracked with SureTrend™ Cloud software for further data analysis and trending.
- Applications: environmental and product testing
- Built-in pipette for easy sample transfer
- Snap-Valve technology – Simply snap & squeeze
- Write-on swab label
- MicroSnap™ Coliform detects:
- E. coli
- Klebsiella
- Citrobacter
- Enterobacter
Same-Shift Results
Reduce Costs
Easy-to-Use Format
Works with Filtered Samples
Works with EnSURE Touch and SureTrend Cloud
Same Shift Results
Rapid test results are essential to releasing product quickly. The MicroSnap™ family of rapid indicator organism tests enables food safety and quality manager to enumerate samples in 6 hours and to determine presence/absence in 8 hours. Simply collect sample, incubate and read with a Hygiena™ Luminometer.
Reduce Costs and Shorten Release Cycles
While sending samples to the lab for PCR analysis can be necessary for a specific application, simpler more cost-effective solutions can supplement your testing protocols. Integrating MicroSnap™ as a rapid indicator test within your testing protocol will reduce material cost, shipping costs and lab fees. Plus, a rapid test also means shorter production hold times and lower inventory holding costs, all leading to more cost savings.
Flexible and Easy-to-Use Format
MicroSnap’s efficient design makes microorganism testing flexible and easy. It can be used for both surfaces and samples. Both options use a simple 2-step process: enrichment and detection. Plus, the patented Snap-Valve holds a precise amount of specific enrichment broth, ready to be used for either product or surface samples. Within the enrichment device is a built-in pipette, enabling convenient and efficient transfer of enriched samples to the detection device. The enriched sample is read on a Hygiena™ luminometer, which means no tedious colony counting is necessary. Use the RLU to CFU conversion table to determine the outcome. Microorganism testing has never been easier.
Works with Filtered Samples
MicroSnap Coliform & E. coli Enrichment Broth is available in a 2mL vial for use with large-volume filtered samples and delivers same-day results. Earlier results mean products can be released sooner; thus cutting inventory and warehouse costs for beverage processors. This method is AOAC approved and validated against the EPA method.
Complete Hygiena Monitoring Program
MicroSnap is an essential component within a complete environmental monitoring program. Combine MicroSnap with the EnSURE Touch and SureTrend Cloud to capture and trend your test results over time. Use the SureTrend Cloud dashboard to see how your ATP testing program impacts your microorganism testing and how the two work together to reduce your risk of a contamination event.
MicroSnap™ Enhanced Nutrient Broth
MicroSnap™ Enhanced Nutrient Broth is a non-selective protein solution. The 9mL nutrient broth enables low-level enumeration when used with MicroSnap™ Total, MicroSnap™ Coliform and MicroSnap™ E. coli. For milk samples, use with MicroSnap Coliform to detect 1 CFU/mL (AOAC-RI Performance Tested Method (Certificate #071302).
Only available by special order in quantities of 25 boxes or more.
MicroSnap Coliform & E.coli with EnSURE Touch
MicroSnap Coliform & E.coli with EnSURE
MicroSnap Coliform for Milk Samples
| Catalog No. | Description | Quantity | |
| MS1-CEC | Coliform & E. coli Enrichment Device | 100 | |
| MS1-CEC-BROTH-2ML | Coliform & E. coli Enrichment Broth (2ml) | 100 | |
| MS1-N-BROTH-9ML | Enhanced Nutrient Broth (9ml) | 100 | |
| MS2-COLIFORM | MicroSnap™ Coliform Detection Device | 100 | |
| MS2-ECOLI | MicroSnap™ E. coli Detection Device | 100 |
MicroSnap E. coli
MicroSnap E. coli is a rapid test for detection and enumeration of E. coli bacteria. The test uses a novel bioluminogenic test reaction that generates light when enzymes that are characteristic of E. coli bacteria react with specialized substrates. The light-generating signal is then quantified in the EnSURE™ Touch luminometer. Depending on the required level of detection, MicroSnap E. coli is able to give results on the same day.
- Applications: environmental and product testing
- Built-in pipette for easy sample transfer
- Snap-Valve technology – Simply snap & squeeze
- Write-on swab label
- MicroSnap™ E. coli detects Escherichia coli bacteria
Same-Shift Results
Reduce Costs
Easy-to-Use Format
Works with Filtered Samples
Works with EnSURE™ Touch and SureTrend™ Cloud
Same Shift Results
Rapid test results are essential to releasing product quickly. The MicroSnap™ family of rapid indicator organism tests enables food safety and quality manager to enumerate samples in 6 hours and to determine presence/absence in 8 hours. Simply collect sample, incubate and read with a Hygiena™ Luminometer.
Reduce Costs and Shorten Release Cycles
While sending samples to the lab for PCR analysis can be necessary for a specific application, simpler more cost-effective solutions can supplement your testing protocols. Integrating MicroSnap™ as a rapid indicator test within your testing protocol will reduce material cost, shipping costs and lab fees. Plus, a rapid test also means shorter production hold times and lower inventory holding costs, all leading to more cost savings.
Flexible and Easy-to-Use Format
MicroSnap’s efficient design makes microorganism testing flexible and easy. It can be used for both surfaces and samples. Both options use a simple 2-step process: enrichment and detection. Plus, the patented Snap-Valve holds a precise amount of specific enrichment broth, ready to be used for either product or surface samples. Within the enrichment device is a built-in pipette, enabling convenient and efficient transfer of enriched samples to the detection device. The enriched sample is read on a Hygiena™ luminometer, which means no tedious colony counting is necessary. Use the RLU to CFU conversion table to determine the outcome. Microorganism testing has never been easier.
Works with Filtered Samples
MicroSnap™ Coliform & E. coli Enrichment Broth is available in a 2mL vial for use with large-volume filtered samples and delivers same-day results. Earlier results mean products can be released sooner; thus cutting inventory and warehouse costs for beverage processors. This method is AOAC approved and validated against the EPA method.
Complete Hygiena Monitoring Program
MicroSnap™ is an essential component within a complete environmental monitoring program. Combine MicroSnap™ with the EnSURE™ Touch and SureTrend™ Cloud to capture and trend your test results over time. Use the SureTrend™ Cloud dashboard to see how your ATP testing program impacts your microorganism testing and how the two work together to reduce your risk of a contamination event.
MicroSnap™ Enhanced Nutrient Broth
MicroSnap™ Enhanced Nutrient Broth is a non-selective protein solution. The 9mL nutrient broth enables low-level enumeration when used with MicroSnap™ Total, MicroSnap™ Coliform and MicroSnap™ E. coli. For milk samples, use with MicroSnap Coliform to detect 1 CFU/mL (AOAC-RI Performance Tested Method (Certificate #071302).
Only available by special order in quantities of 25 boxes or more.
MicroSnap Coliform & E.coli with EnSURE Touch
MicroSnap Coliform & E.coli with EnSURE
MicroSnap Coliform for Milk Samples
| Catalog No. | Description | Quantity | |
| MS1-CEC | Coliform & E. coli Enrichment Device | 100 | |
| MS1-CEC-BROTH-2ML | Coliform & E. coli Enrichment Broth (2ml) | 100 | |
| MS2-COLIFORM | MicroSnap™ Coliform Detection Device | 100 | |
| MS2-ECOLI | MicroSnap™ E. coli Detection Device | 100 |
MicroSnap EB
MicroSnap EB is a rapid test for detection and enumeration of Enterobacteriaceae (EB) bacteria. The test uses a novel bioluminogenic test reaction that generates light when enzymes that are characteristic of EB bacteria react with specialized substrates to produce light. The light-generating signal is then quantified in the EnSURE™ Touch luminometer. Depending on the required level of detection, MicroSnap™ EB is able to give results within the same day.
- Applications: environmental and product testing
- Built-in pipette for easy sample transfer
- Snap-Valve technology – snap & squeeze
- Write-on swab label
- MicroSnap™ EB detects:
- E. coli
- Klebsiella
- Citrobacter
- Enterobacter
- Serratia
- Shigella
- Salmonella
- Yersinia
Same-Shift Results
Reduce Costs
Easy-to-Use Format
Works with Filtered Samples
Works with EnSURE™ Touch and SureTrend™ Cloud
Same Shift Results
Rapid test results are essential to releasing product quickly. The MicroSnap™ family of rapid indicator organism tests enables food safety and quality manager to enumerate samples in 6 hours and to determine presence/absence in 8 hours. Simply collect sample, incubate and read with a Hygiena™ Luminometer.
Reduce Costs and Shorten Release Cycles
While sending samples to the lab for PCR analysis can be necessary for a specific application, simpler more cost-effective solutions can supplement your testing protocols. Integrating MicroSnap™ as a rapid indicator test within your testing protocol will reduce material cost, shipping costs and lab fees. Plus, a rapid test also means shorter production hold times and lower inventory holding costs, all leading to more cost savings.
Flexible and Easy-to-Use Format
The efficient design of MicroSnap™ makes microorganism testing flexible and easy. It can be used for both surfaces and samples. Both options use a simple 2-step process: enrichment and detection. Plus, the patented Snap-Valve holds a precise amount of specific enrichment broth, ready to be used for either product or surface samples. Within the enrichment device is a built-in pipette, enabling convenient and efficient transfer of enriched samples to the detection device. The enriched sample is read on Hygiena™ luminometer, which means no tedious colony counting necessary. Use the RLU to CFU conversion table to determine the outcome. Microorganism testing has never been easier.
Complete Hygiena Monitor Program
MicroSnap™ is an essential component within a complete environmental monitoring program. Combine MicroSnap with the EnSURE™ Touch and SureTrend™ Cloud to capture and trend your test results over time. Use SureTrend Cloud dashboard to see how your ATP testing program impacts your microorganism testing and how the two work together to reduce your risk of an event.
MicroSnap™ Enhanced EB Broth
MicroSnap™ Enhanced EB is a non-selective protein solution. The 9mL broth reduces interference from physical components in the sample and enables low-level enumeration when used with MicroSnap™ EB, MicroSnap™ Coliform and MicroSnap™ E. coli. Just add 1mL of sample to the 9mL volume and incubate!
Only available by special order in quantities of 25 boxes or more.
MicroSnap™ Total & EB with EnSURE™ Touch
MicroSnap™ EB with EnSURE™
| Catalog No. | Description | Quantity | |
| MS1-EB | MicroSnap™ EB Enrichment Device | 100 | |
| MS2-EB | MicroSnap™ EB Detection Device | 100 |
MicroSnap Total
MicroSnap Total delivers same-day total viable count (TVC) test results, giving you the advantage of knowing microbial levels on the same day samples are collected. The test uses a novel bioluminogenic test reaction that generates light when enzymes that are characteristic of viable bacteria react with substrates to produce light. The light-generating signal is then quantified with a Hygiena™ luminometer. With same-day results, MicroSnap™ Total results enable you to take corrective actions faster or release products sooner. AOAC-RI Performance Tested Method (Certification #031501).
- Snap-Valve technology – snap & squeeze
- Write-on swab label
- For use with EnSURE luminometer
- AOAC-RI Performance Tested Method, Certificate No. 031501
Same-Shift Results
Reduce Costs
Easy-to-Use Format
Works with Filtered Samples
Works with EnSURE Touch and SureTrend Cloud
Same Shift Results
Rapid test results are essential to releasing product quickly. The MicroSnap™ family of rapid indicator organism tests enables food safety and quality manager to enumerate samples in 6 hours and to determine presence/absence in 8 hours. Simply collect sample, incubate and read with a Hygiena™ Luminometer.
Reduce Costs and Shorten Release Cycles
While sending samples to the lab for PCR analysis can be necessary for a specific application, simpler more cost-effective solutions can supplement your testing protocols. Integrating MicroSnap™ as a rapid indicator test within your testing protocol will reduce material cost, shipping costs and lab fees. Plus, a rapid test also means shorter production hold times and lower inventory holding costs, all leading to more cost savings.
Flexible and Easy-to-Use Format
MicroSnap’s efficient design makes microorganism testing flexible and easy. It can be used for both surfaces and samples. Both options use a simple 2-step process: enrichment and detection. Plus, the patented Snap-Valve holds a precise amount of specific enrichment broth, ready to be used for either product or surface samples. Within the enrichment device is a built-in pipette, enabling convenient and efficient transfer of enriched samples to the detection device. The enriched sample is read on Hygiena™ luminometer, which means no tedious colony counting necessary. Use the RLU to CFU conversion table to determine the outcome. Microorganism testing has never been easier.
Complete Hygiena Monitor Program
MicroSnap is an essential component within a complete environmental monitoring program. Combine MicroSnap with the EnSURE™ Touch and SureTrend™ Cloud to capture and trend your test results over time. Use SureTrend Cloud dashboard to see how your ATP testing program impacts your microorganism testing and how the two work together to reduce your risk of an event.
MicroSnap™ Enhanced Nutrient Broth
MicroSnap™ Enhanced Nutrient Broth is a non-selective protein solution. The 9mL nutrient broth enables low-level enumeration when used with MicroSnap™ Total, MicroSnap™ Coliform and MicroSnap™ E. coli. For milk samples, use with MicroSnap Coliform to detect 1 CFU/mL (AOAC-RI Performance Tested Method (Certificate #071302).
Only available by special order in quantities of 25 boxes or more.
MicroSnap™ Total & EB with EnSURE™ Touch
MicroSnap™ Total with EnSURE™
| Catalog No. | Description | Quantity | |
| MS1-TOTAL | MicroSnap™ Total Enrichment Device | 100 | |
| MS2-TOTAL | MicroSnap™ Total Detection Device | 100 |
InSite Listeria
Environmental Listeria species Test
Hygiena InSite Listeria is an easy-to-use, self-contained, environmental Listeria species test. Each device contains a chromogenic liquid media formulated with antibiotics, growth enhancers, and color-changing compounds specific to Listeria species. Plus, all InSite™ test devices contain neutralizers in the wetting solution to help combat potential sanitizer side effects and improve sample collection. Simply swab the test area and wait. A change in color after 24-48 hours of incubation is considered a presumptive positive. No expensive lab equipment is required and no additional sample handling of the sample is necessary.
- Snap-Valve technology – simply snap & squeeze
- Large 2.5 inch foam swab for the maximum sample pickup
- Write-on swab label for easy sample identification
- Presumptive positive results are available for the most common Listeria spp in as little as 30 hours at levels as low as 1-10 CFU/mL
- Presumptive positive samples should be confirmed by streaking samples onto commonly used selective Listeria agar plates such as Modified Oxford agar, Palcam agar, or any other recognized confirmatory procedure. Typical Listeria colonies on selective agar plates could then be further analyzed by more definitive tests such as microscopy, biochemical tests, etc.
- Compared to UVM and BLEB, provides superior/equivalent recovery and faster detection of as low as 10-50 heat injured Listeria monocytogenes/mL within ~24-30 hours of incubation.
- Provides a higher level of selectivity and sensitivity for heat injured L. monocytogenes under binary competition conditions compared to BLEB and UVM media
Results in
24-48 Hours
Reduce
Material & Labor
Self-Contailed
Reagent
Easy to Interpret
Color Change
Patented Snap-Valve Technology
How to Use
| Catalog No. | Description | Quantity | |
| ILC50 | InSite™ Listeria Test (box of 50) | 50 Tests | |
| ILC100 | InSite™ Listeria Test (box of 100) | 100 Tests |
InSite L. mono Glo
Environmental L. mono Test
Hygiena InSite L. mono Glo is an easy-to-use, self-contained, environmental sampling and screening test for Listeria species and Listeria monocytogenes (L. mono). Each device contains a chromogenic liquid media formulated with antibiotics, growth enhancers, and color-changing compounds specific to Listeria plus fluorescent compounds specific to Listeria monocytogenes. Plus, all InSite™ test devices contain neutralizers in the wetting solution to help combat potential sanitizer side effects and improve sample collection. The two-step test changes color in the presence of Listeria species, while illumination with UV light reveals the presence of L.mono.
- Snap-Valve technology – simply snap & squeeze
- Large 2.5 inch foam swab for the maximum sample pickup
- Write-on swab label for easy sample identification
- Presumptive positive results are available for the most common Listeria spp in as little as 24 hours at levels as low as 1-10 CFU/mL
- Presumptive positive samples should be confirmed by an alternative method such as PCR, lateral flow, or traditional bacteriology methods. Any confirmatory results should be handled according to appropriate regulations.
- Compared to UVM and BLEB, provides superior/equivalent recovery and faster detection of as low as 10-50 heat-injured Listeria monocytogenes within ~24-48 hours of incubation
Results in 24-48 Hours
Reduce Material & Labor
Self-Contained Reagent
Easy to Interpret Color Change
Patented Snap-Valve Technology
Easy to Interpret Color Results
It doesn’t take a microbiologist to understand these results. When Listeria species are present in a sample, the liquid media changes color from amber to black. If pathogenic Listeria, like L. mono, is present, the media will glow green under UV light.
| Catalog No. | Description | Quantity | |
| ILM050 | InSite™ L. mono GloTest | 50 |
InSite Salmonella
Environmental Salmonella Test
Hygiena InSite Salmonella is an easy-to-use, self-contained, environmental Salmonella spp. test. Each device contains a liquid medium formulated with growth enhancers and chromogenic compounds selective for Salmonella species. All InSite™ test devices contain neutralizers in the wetting solution to help combat potential sanitizer side effects and improve sample collection. Simply swab the test area and incubate. A change in color after 24-48 hours of incubation is considered presumptive positive for Salmonella species. No expensive lab equipment is required.
The InSite™ Chromo and InSite™ L. mono Glo test devices contain a neutralizing wetting solution on the swab, it is a mixture of Buffered Peptone Water and Neutralizing Broth.
- Snap-Valve technology – Simply snap & squeeze
- Large 2.5 inch foam swab for the maximum sample pickup
- Write-on swab label for easy sample identification
- Presumptive positive results are available in as little as 24 hours
- Presumptive positive samples can be confirmed by streaking samples onto commonly used selective Salmonella agar plates or any recognized confirmatory procedure. Typical Salmonella colonies on selective agar plates could then be further analyzed by using biochemical, immunological, or molecular methods.
- Sensitive to 1-10 CFU
Results in 24-48 Hours
Reduce Material & Labor
Self-Contained Reagent
Easy to Interpret Color Change
Patented Snap-Valve Technology
How to Use
Easy to Interpret Color Results
It doesn’t take a microbiologist to understand these results. When Salmonella species is present in a sample, the liquid medium changes color from purple to vivid yellow.
| Catalog No. | Description | Quantity | |
| IS050 | Insite Salmonella | 50 |
Digital Dry Block Incubators
Designed specifically for Hygiena’s wide variety of test devices, our Digital Dry Block Incubators are flexible to fit your unique needs. Incubators are available in two different sizes and multiple configurations.
- Wide temperature range:
- Room temp +5 – 80 °C for Small Format Incubator
- Room temp +5 – 105 °C for Lab Format Incubator
- Footprint / Weight:
- Small Format (W x D x H): 11 cm x 15 cm x 8 cm, 0.5 kg / 4.3 in x 5.9 in x 3.1 in, 1.1 lbs
- Lab Format (W x D x H): 36.5 cm x 21 cm x 15 cm, 4.5 kg / 14.4 in x 8.3 in x 5.9 in, 9.9 lbs
- Temperature stability ≤ ± 0.5 °C
- Volumetric capacity of small tube incubator blocks = 1.5 mL
- Heats to max temp in less than 15 minutes
- Timer range: 1 min – 99 hr 59 min
- Blocks are sold separately – see Catalog No’s for ordering information
- Includes manual, power cord, wrench, and performance certificate
Dual Temperature Control
Multiple Configurations
Numbered Wells for Easy Tracking
Small Footprint
Wide Temperature Range
Two Sizes to Fit Your Needs
Digital Dry Block Incubators are available in two sizes to accommodate your testing volume. The small footprint version is perfect for labs with limited bench-top space and small test volumes (6-12 wells). The larger lab format incubator (30-70 wells) offers two separate blocks (A & B) each with separate temperature controls and allowing for three separate well configurations.
Customize Block Configuration
The Lab Format Digital Dry Block Incubator can be configured in three different ways to fit your testing needs. Since blocks are heated independently, you can run multiple test assays all on one incubator. Blocks are sold separately.
Digitally controlled time and temperature.
Capable of up to 105 °C, Digital Dry Block Incubators can be set to any temperature required by Hygiena™ test devices, and more! The digitally controlled blocks are independently heated, allowing you to run two temperatures in one incubator at a time – ideal for users running a variety of tests. A built-in timer features auto-stop and sound alert to help you achieve perfect incubation, every time.
| Catalog No. | Description | Quantity | |
| INCUBATOR | Small Incubator (blocks sold separately) | 1 | |
| IB003 | 12-well block for small incubator (Model No: INCUBATOR) | 1 | |
| IB004 | 6-well block for small incubator (Model No: INCUBATOR) | 1 | |
| INCUBATOR2 | Lab Format Incubator (blocks sold separately) | 1 | |
| IB001 | 35-well swab-size block for INCUBATOR2 | 1 | |
| IB002 | 15-well vial tube size block for INCUBATOR2 | 1 |
