Culture Media
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Tryptic Soy Broth, Modified with Novobiocin
Tryptic Soy Broth, Modified with Novobiocin is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. Tryptic Soy Broth, Modified with Novobiocin is not intended for use in the diagnosis of disease or other conditions in humans.
The first major outbreak of E. coli O157:H7 was in 1982 and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water.
Tryptic Soy Broth, Modified with Novobiocin is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.
| Brand | Neogen® Culture Media | ||||||||||||||||
| Analyte | Enterohemorrhagic | ||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||
| Mass | 5 kg | ||||||||||||||||
| Formula |
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| Grams per Liter | 33.00 | ||||||||||||||||
| Liters per 500 g | 15.00 | ||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7694 Tryptic Soy Broth, Modified with Novobiocin | ||||||||||||||||
| Package Weight | 14.00 lb |
KF Streptococcus Agar
KF Streptococcus Agar is used with triphenyltetrazolium chloride for the selective isolation and enumeration of fecal streptococci in a laboratory setting. KF Streptococcus Agar is not intended for use in the diagnosis of disease or other conditions in humans.
KF Streptococcus Agar was developed by Kenner, Clark, and Kabler for the detection of fecal streptococci in surface waters. KF Streptococcus Agar is currently used in detecting fecal streptococci in water, foods, and other materials through the membrane filtration technique or pour plate method. Fecal streptococci are normal inhabitants in the intestines, and the presence of fecal streptococci can be used as an indication of fecal contamination. The presence of fecal streptococci is of value in determining pollution sources because certain fecal streptococci are host-specific.
| Brand | Neogen® Culture Media | ||||||||||||||||||||
| Analyte | Faecal Streptococci | ||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||
| Formula |
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| Grams per Liter | 76.40 | ||||||||||||||||||||
| Liters per 500 g | 7.00 | ||||||||||||||||||||
| Plates per 500 g | 360.00 | ||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7610 KF Streptococcus Agar | ||||||||||||||||||||
| Package Weight | 14.00 lb |
Sabouraud Dextrose Agar, Emmons
Sabouraud Dextrose Agar, Emmons is used for the cultivation of fungi in a laboratory setting. Sabouraud Dextrose Agar, Emmons is not intended for use in the diagnosis of disease or other conditions in humans.
Sabouraud Dextrose Agar (SDA), Emmons is a modification of Dextrose Agar described by Sabouraud. SDA is used for cultivating pathogenic and commensal fungi and yeasts. Emmons modified the formula by adjusting the pH and reducing the dextrose concentration. This neutral pH enhances recovery of some pathogenic fungi. Sabouraud Dextrose Agar, Emmons is an excellent basal medium, and antibiotics and inhibitors may be added for the selective cultivation and isolation of various microorganisms.
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Fungi | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 10 kg | ||||||||||
| Formula |
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| Grams per Liter | 47.00 | ||||||||||
| Liters per 500 g | 11.00 | ||||||||||
| Plates per 500 g | 580.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7204 Sabouraud Dextrose Agar, Emmons | ||||||||||
| Package Weight | 26.60 lb |
M-Green Yeast and Fungi Broth
M-Green Yeast and Fungi Broth is used for the detection of fungi in beverages in a laboratory setting. M-Green Yeast and Fungi Broth is not intended for use in the diagnosis of disease or other conditions in humans.
M-Green Yeast and Fungi Broth is a relatively more complex formula compared to other media used for isolation of fungi and yeast. This formulation is rich in nutrients which allows for excellent fungal growth. Bacterial growth is inhibited by an acid pH.
Fungi have been found in potable water and on the inner surface of distribution system pipes. They can survive water treatment or they enter the system after treatment and remain viable.
| Brand | Neogen® Culture Media | ||||||||||||||||||||
| Analyte | Fungi | ||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||
| Mass | 10 kg | ||||||||||||||||||||
| Formula |
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| Grams per Liter | 73.00 | ||||||||||||||||||||
| Liters per 500 g | 7.00 | ||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7190 m-Green Yeast and Fungi Broth | ||||||||||||||||||||
| Package Weight | 26.60 lb |
Sabouraud Dextrose Agar with Lecithin & Tween
Sabouraud Dextrose Agar (with Lecithin & Tween) is used for the isolation of fungi from surfaces sanitized with quaternary ammonium compounds in a laboratory setting. Sabouraud Dextrose Agar (with Lecithin & Tween) is not intended for use in the diagnosis of disease or other conditions in humans.
Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. SDA is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permits selectivity of fungi. The addition of Lecithin and Tween to Sabouraud Dextrose Agar is used to neutralize antiseptics and disinfectants for environmental monitoring and other applications. Complete neutralization of disinfectants is important. Disinfectant carryover can cause false no-growth test results.
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Fungi | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 71.00 | ||||||||||||||
| Liters per 500 g | 7.00 | ||||||||||||||
| Plates per 500 g | 380.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7392 Sabouraud Dextrose Agar w/ Lecithin & Tween | ||||||||||||||
| Package Weight | 14.00 lb |
Sabouraud Dextrose Agar with Chloramphenicol
Sabouraud Dextrose Agar with Chloramphenicol is used for the selective isolation of fungi in a laboratory setting. Sabouraud Dextrose Agar with Chloramphenicol is not intended for use in the diagnosis of disease or other conditions in humans.
Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. Sabouraud Dextrose Agar is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permits selectivity of fungi. This medium is beneficial in sporulation studies and pigment production. Sabouraud Dextrose Agar is used for determining the microbial content of cosmetics and in the mycological evaluation of food.
Sabouraud Dextrose Agar with Chloramphenicol is a modification of Sabouraud Dextrose Agar, with the addition of Chloramphenicol to increase selectivity against commensal microorganisms.
| Brand | Neogen® Culture Media | ||||||||||||
| Analyte | Fungi | ||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||
| Mass | 5 kg | ||||||||||||
| Formula |
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| Grams per Liter | 65.00 | ||||||||||||
| Liters per 500 g | 8.00 | ||||||||||||
| Plates per 500 g | 420.00 | ||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7306 Sabouraud Dextrose Agar with Chloramphenicol | ||||||||||||
| Package Weight | 14.00 lb |
Mycobiotic Agar
Mycobiotic Agar is used for the selective isolation of fungi in a laboratory setting. Mycobiotic Agar is not intended for use in the diagnosis of disease or other conditions in humans.
The value of selective media for initial cultivation of pathogenic fungi has been demonstrated by numerous investigators. Historically, media for fungi generally relied on an acid pH to make the media less suitable for growth of many bacteria. Recently developed media use neutral or slightly alkaline reactions, antibiotics, bile salts, and dyes as selective agents against bacteria. Mycobiotic Agar is an excellent basal medium and antifungal agents, cycloheximide and chloramphenicol, are added to study their affect on fungi. This medium is proven useful in the isolation of dermatophytes and other fungi from samples.
George recommends the use of Mycobiotic Agar exclusively for isolating dermatophytes (dermatophytes are not sensitive to cycloheximide or chloramphenicol) and in parallel to media without antibiotics for isolating fungi which cause systemic disease.
| Brand | Neogen® Culture Media | ||||||||||||
| Analyte | Fungi | ||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||
| Mass | 500 g | ||||||||||||
| Formula |
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| Grams per Liter | 35.50 | ||||||||||||
| Liters per 500 g | 14.00 | ||||||||||||
| Plates per 500 g | 774.00 | ||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7419 Mycobiotic Agar | ||||||||||||
| Package Weight | 15.30 lb |
Potato Dextrose Broth
Potato Dextrose Broth is used for the cultivation of fungi in a laboratory setting. Potato Dextrose Broth is not intended for use in the diagnosis of disease or other conditions in humans.
Potato Dextrose Broth is a general purpose broth for yeasts and molds. The low pH of this medium inhibits bacterial growth. Potato Dextrose Broth is the same formula as Potato Dextrose Agar, but agar has been omitted.
| Brand | Neogen® Culture Media | ||||||
| Analyte | Fungi | ||||||
| Platform | Dehydrated Culture Media | ||||||
| Mass | 5 kg | ||||||
| Formula |
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| Grams per Liter | 24.00 | ||||||
| Liters per 500 g | 21.00 | ||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7585 Potato Dextrose Broth | ||||||
| Package Weight | 14.00 lb |
YM Broth
YM Broth is used for the cultivation of fungi in a laboratory setting. YM Broth is not intended for use in the diagnosis of disease or other conditions in humans.
YM Broth is prepared according to the formulation published by Wickerham. Wickerham suggested that YM Broth, acidified to pH 3.0 – 4.0, be used as an enrichment medium for yeasts from populations also containing bacteria and molds. To favor isolation of fermentative species, add a layer of sterile paraffin oil 1 cm deep on the surface of the inoculated broth. Cultures should be incubated until growth appears, and then streaked onto YM Agar to obtain isolated yeast colonies. To isolate fermentative and oxidative strains, place acidified inoculated YM Broth on a rotary shaker for 1 to 2 days. This favors yeast recovery while preventing sporulation of molds.
Media selectivity may be enhanced through acidification or addition of selective agents. YM Broth may be acidified prior to sterilization.
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Fungi | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 500 g | ||||||||||
| Formula |
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| Grams per Liter | 21.00 | ||||||||||
| Liters per 500 g | 24.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7363 YM Broth | ||||||||||
| Package Weight | 1.35 lb |
Dextrose, Anhydrous
Dextrose is used in preparing microbiological culture media in a laboratory setting. Dextrose is not intended for use in the diagnosis of disease or other conditions in humans.
A basic characteristic of a microorganism is its nutritional requirements and without the proper nutrients the organism cannot survive. An essential variety of ingredients are used in the preparation of dehydrated culture media to ensure optimal growth of the microorganism. Carbohydrates, including Dextrose, play an important role as an energy source to increase the growth of bacteria and fungi.
| Brand | Neogen® Culture Media |
| Analyte | Fungi |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Grams per Liter | 39.00 |
| Liters per 500 g | 13.00 |
| Plates per 500 g | 700.00 |
| Previous Item Number | Product was previously known as Acumedia Product: 7203 Dextrose |
| Package Dimensions | 11.50 in x 11.50 in x 10.50 in |
| Package Weight | 14.00 lb |
Agar No. 4 - Plant Tissue Culture Grade
Agar No.4 has been selected specifically for use as a gelling agent in plant tissue culture techniques, and is not intended for use in the diagnosis of disease or other conditions in humans. Agar No.4 is selected primarily on gel strength, a parameter of particular importance for plant tissue culture techniques, and then tested to ensure it meets the parameters set by a major Horticultural company. The agar contains no nutrients for plant growth and is designed to be incorporated into classical formulations such as Murashige and Skoog, as well as customer’s own formulations.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC029 Agar No 4 Plant Tissue Culture Grade |
| Package Weight | 15.30 lb |
Casein Digest
Casein Digest is an enzymatic digest of casein for use in preparing microbiological culture media in a laboratory setting. Casein Digest is not intended for use in the diagnosis of disease or other conditions in humans.
Casein is the main protein of milk and a rich source of amino nitrogen. Casein Digest was developed for use in molecular genetic media formulations, including NZCYM Broth, NZYM Broth, and NZM Broth. The enriched formulations of NZ media are used to cultivate recombinant strains of E. coli and propagating theta bacteriophages.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 500 g |
| Grams per Liter | 35.00 |
| Liters per 500 g | 14.00 |
| Plates per 500 g | 780.00 |
| Previous Item Number | Product was previously known as Acumedia Product: 7689 Casein Digest Peptone |
| Package Weight | 1.35 lb |
Bile Salts No. 3
Bile Salts No. 3 is a mixture of bile salts for use in preparing microbiological culture media in a laboratory setting. Several media containing Bile Salts No. 3 are recommended in standard methods for multiple applications. Bile Salts No. 3 is not intended for use in the diagnosis of disease or other conditions in humans.
Bile Salts No. 3 is a refined bile salts mixture, comprising mainly sodium cholate and sodium desoxycholate. Its primary use is as a selective agent for the isolation of Gram-negative micro-organisms, inhibiting Gram-positive cocci. Bile Salts No. 3 is prepared especially for use in MacConkey Agar and Violet Red Bile Agar. This fraction of bile is highly active allowing maximum selection of organisms of enteric origin at relatively low concentrations (0.15%) and is soluble in distilled or deionized water and neutral to slightly alkaline in pH.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7320 Bile Salts No. 3; and LabM Product: LABMC025 Bile Salts No. 3 |
| Package Weight | 14.00 lb |
Agar, Bacteriological No. 1
Agar, Bacteriological No. 1 is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Bacteriological No. 1 is not intended for use in the diagnosis of disease or other conditions in humans. Agar, Bacteriological No. 1 conforms with the testing specified in the USP/EP Pharmacopeia.
Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperature for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.
Agar is a gel at room temperature, remaining firm at temperatures as high as 65°C. Agar melts at approximately 85 – 91°C, a different temperature from solidification at 34 – 36°C. This property is known as hysteresis. Agar is generally resistant to shear forces; however, different agar may have different gel strengths or degrees of stiffness.
Specifications for Agar, Bacteriological No. 1 include good clarity, controlled gelation temperature, controlled melting temperature, good diffusion characteristics, absence of toxic bacterial inhibitors, and relative absence metabolically useful minerals and compounds. Agar, Bacteriological No. 1 is recommended for clinical applications, auxotrophic studies, bacterial and yeast transformation studies, and bacterial molecular genetics applications.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7178 Agar, Bacteriological |
| Package Weight | 14.00 lb |
Agar, Bacteriological No. 2
Agar, Bacteriological No. 2 is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Bacteriological No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.
Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperature for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.
Agar is a gel at room temperature, remaining firm at temperatures as high as 65°C. Agar melts at approximately 85 – 91°C, a different temperature from solidification at 34 – 36°C. This property is known as hysteresis. Agar is generally resistant to shear forces; however, different agar may have different gel strengths or degrees of stiffness.
Specifications for Agar, Bacteriological No. 2 include good clarity, controlled gelation temperature, controlled melting temperature, good diffusion characteristics, absence of bacterial inhibitors, and the presence of metabolically useful minerals such as calcium and magnesium. Agar, Bacteriological No. 2 is recommended for various applications, auxotrophic studies, bacterial and yeast transformation studies, and bacterial molecular genetics applications.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7558 Agar, Bacteriological No. 2 |
| Package Weight | 14.00 lb |
Malt Extract
Malt Extract is a dehydrated extract of malt for use in preparing microbiological culture media in a laboratory setting. Malt Extract is not intended for use in the diagnosis of disease or other conditions in humans.
Malt Extract is a clarified, water-soluble extract of malted barley. Malt Extract is a useful ingredient of culture media designed for the propagation of yeasts and molds. This ingredient is suitable for yeasts and molds because it contains a high concentration of carbohydrates, particularly maltose. The approximate percentage of reducing sugars in Malt Extract is 60 – 63%.The high carbohydrate content makes malt extract very sensitive to overheating which results in darkening of the medium it is used in. Malt Extract is generally employed in culture media at concentrations between 10 to 100 grams per liter.
Malt Agar, a medium recommended for the detection and isolation of yeast and molds from dairy products, food, and as a stock culture, contains Malt Extract. Wort Agar, used for the cultivation and enumeration of yeasts, has Malt Extract as one of the main ingredients in the formula. Several media containing Malt Extract are specified in standard methods.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 10 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7341 Malt Extract; and LabM Product: LABMC023 Malt Extract |
| Package Weight | 28.00 lb |
Agar No. 4 - Plant Tissue Culture Grade
Agar No.4 has been selected specifically for use as a gelling agent in plant tissue culture techniques, and is not intended for use in the diagnosis of disease or other conditions in humans. Agar No.4 is selected primarily on gel strength, a parameter of particular importance for plant tissue culture techniques, and then tested to ensure it meets the parameters set by a major Horticultural company. The agar contains no nutrients for plant growth and is designed to be incorporated into classical formulations such as Murashige and Skoog, as well as customer’s own formulations.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC029 Agar No 4 Plant Tissue Culture Grade |
| Package Weight | 15.30 lb |
Liver Digest
Liver Digest is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.
Liver digest is prepared by the controlled hydrolysis of liver. It is rich in vitamins and essential amino acids and has excellent nutritional properties and especially favors the growth of strict anaerobes and other fastidious microorganisms. Due to its capacity to stimulate sugar metabolism in saccharolytic organisms it is perfectly suited for the growth of a broad range of organisms such as Clostridium, Leuconostoc, Bacillus, homo and hetero-fermentative Lactic acid bacteria, as well as yeasts and filamentous molds.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 500 g |
| Previous Item Number | Product was previously known as LabM Product: MC034 Liver Digest |
| Package Weight | 1.35 lb |
Oxbile (Oxgall)
Oxbile (Oxgall) is dehydrated bile for use in preparing microbiological culture media in a laboratory setting. Oxbile (Oxgall) is not intended for use in the diagnosis of disease or other conditions in humans.
Oxbile is manufactured from large quantities of fresh bile by rapid evaporation of the water content. Bile is composed of fatty acids, bile acids, inorganic salts, sulfates, bile pigments, cholesterol, mucin, lecithin, glycuronicacids, porphyrins, and urea. The use of Oxbile insures a regular supply of bile, and uniformity impossible to obtain with fresh materials.
Oxbile is dehydrated fresh bile and prepared specifically for differentiation of bile tolerant microorganisms. A 10% solution of dehydrated bile is equivalent to a fresh bile solution. It is usually incorporated into media e.g., Bile Esculin Agar and Brilliant Green Bile Agar, used for the determination of enteric pathogens. Oxbile is also found in Littman Agar, a selective fungal medium.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 500 g |
| Previous Item Number | Product was previously known as Acumedia Product: 7216 Oxbile (Oxgall) |
| Package Weight | 1.35 lb |
Lactose
Lactose is used in preparing microbiological culture media in a laboratory setting. Lactose is not intended for use in the diagnosis of disease or other conditions in humans.
A basic characteristic of a microorganism is its nutritional requirements, and without the proper nutrients the organism cannot survive. An essential variety of ingredients are used in the preparation of dehydrated culture media to ensure optimal growth of the microorganism. Carbohydrates, including lactose, play an important role as an energy source to increase the growth of bacteria and fungi.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 10 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7231 Lactose; and LabM Product: LABMC020 Lactose |
| Package Weight | 26.60 lb |
Agar No. 1 Bacteriological
Agar No. 1 Bacteriological is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.
A high clarity agar with good gelling properties and a low concentration of metal ions. This agar is suitable for all bacteriological purposes including sensitivity testing and pour plate techniques. A firm gel is obtained at working concentrations of 1.0 to 1.5%. No significant precipitation is observed on reheating or prolonged holding at 65°C.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 10 kg |
| Previous Item Number | Product was previously known as LabM Product: MC002 Agar No 1 Bacteriological |
| Package Weight | 26.60 lb |
Agar, Bacteriological No. 2
Agar, Bacteriological No. 2 is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Bacteriological No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.
Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperature for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.
Agar is a gel at room temperature, remaining firm at temperatures as high as 65°C. Agar melts at approximately 85 – 91°C, a different temperature from solidification at 34 – 36°C. This property is known as hysteresis. Agar is generally resistant to shear forces; however, different agar may have different gel strengths or degrees of stiffness.
Specifications for Agar, Bacteriological No. 2 include good clarity, controlled gelation temperature, controlled melting temperature, good diffusion characteristics, absence of bacterial inhibitors, and the presence of metabolically useful minerals such as calcium and magnesium. Agar, Bacteriological No. 2 is recommended for various applications, auxotrophic studies, bacterial and yeast transformation studies, and bacterial molecular genetics applications.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7558 Agar, Bacteriological No. 2 |
| Package Weight | 14.00 lb |
Tryptone No. 1
Tryptone No. 1 is an enzymatic digest of casein and meat for use in preparing microbiological culture media in a laboratory setting. Tryptone No. 1 is not intended for use in the diagnosis of disease or other conditions in humans.
Tryptone No. 1 is an enzymatic digest of casein and meat used as a nitrogen source in culture media. Casein is the main protein of milk, and a rich source of amino-acid nitrogen. Tryptone No. 1 is rich in tryptophane, making it valuable for use in detecting indole production. The absence of detectable levels of carbohydrates in Tryptone makes it a suitable peptone in differentiating bacteria on the basis of their ability to ferment various carbohydrates.
Several media containing Tryptone are specified in standard methods for multiple applications.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 10 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7351 Tryptone |
| Package Weight | 26.60 lb |
Pancreatic Digest of Gelatin
Pancreatic Digest of Gelatin is an enzymatic digest of gelatin for use in preparing microbiological culture media in a laboratory setting. Pancreatic Digest of Gelatin is not intended for use in the diagnosis of disease or other conditions in humans.
Pancreatic Digest of Gelatin provides a complex nitrogen source and is used as a media ingredient for fermentation studies. Pancreatic Digest of Gelatin alone is used to support the growth of non-fastidious microorganisms. Pancreatic Digest of Gelatin is deficient in carbohydrates and distinguished by low cystine and tryptophan content.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 9190 Pancreatic Digest of Gelatin |
| Package Weight | 14.00 lb |
Agar, Technical
Agar, Technical is a solidifying agent for use in preparing microbiological culture media in a laboratory setting. Agar, Technical is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to US Pharmacopeia (USP).
Agar is a phycocolloid extracted from a group of red-purple algae, usually Gelidium spp. Agar was first suggested for microbiological purposes in 1881 by Fannie Hesse. By the early 1900’s, agar became the gelling agent of choice over gelatin because agar remains firm at growth temperatures for many pathogens and agar is generally resistant to a breakdown by bacterial enzymes. The use of agar in microbiological media significantly contributed to the advance of microbiology, paving the way to study pure cultures.
Agar is a gel at room temperature, remaining firm at temperatures as high as 65°C. Agar melts at approximately 85 – 91°C, a different temperature from solidification at 34 – 36°C. This property is known as hysteresis. Agar is generally resistant to shear forces; however, different agar may have different gel strengths or degrees of stiffness.
Specifications for Agar, Technical include good clarity, controlled gelation temperature, controlled melting temperature, good diffusion characteristics, absence of toxic bacterial inhibitors, and relative absence of metabolically useful minerals and compounds. Agar, Technical is recommended as a general purpose agar for preparing microbiological culture media.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 500 g |
| Previous Item Number | Product was previously known as Acumedia Product: 7619 Agar, Technical |
| Package Weight | 1.35 lb |
Meat Peptone
Meat Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.
Meat Peptone is a highly nutritious enzymatic digest of meat and may be used as an ingredient in culture media.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC018 Meat Peptone |
| Package Weight | 15.30 lb |
Soy Peptone
Soy Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.
Soy peptone is a rich source of nutrients with a high carbohydrate content. Most organisms will grow rapidly in this peptone but some bacteria will produce high levels of acid leading to auto-sterilization unless an adequate buffering system is incorporated.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 500 g |
| Previous Item Number | Product was previously known as LabM Product: MC003 Soy Peptone |
| Package Weight | 1.35 lb |
Bacteriological Peptone
Bacteriological Peptone is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.
An economical source of nutrients provided by a balanced mixture of meat peptones and tryptone. The growth requirements of most non-fastidious organisms will be fulfilled by the range of amino acids, peptides and proteoses in this mixture.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC024 Bacteriological Peptone |
| Package Weight | 15.30 lb |
Meat Peptone No. 1
Meat Peptone No.1 is an enzymatic digest of animal tissue used in preparing microbiological culture media in a laboratory setting. Meat Peptone No.1 is not intended for use in the diagnosis of disease or other conditions in humans.
Meat Peptone No. 1 was developed as an alternate nitrogen and vitamin source in dehydrated culture media to accommodate varying nutritional requirements.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 10 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7181 Peptic Digest of Animal Tissue (Peptone A) |
| Package Weight | 26.60 lb |
Beef Extract Powder
Beef Extract Powder is a dehydrated extract of bovine tissue for use in preparing microbiological culture media in a laboratory setting. Beef Extract Powder is not intended for use in the diagnosis of disease or other conditions in humans.
Beef Extract Powder is prepared and standardized for use in microbiological culture media, where it is generally used to replace infusion of meat. Culture media containing Beef Extract Powder are recommended for use in bacteriological examination of water, milk, and other materials, where uniform composition of media is important. Beef Extract Powder is relied upon for biochemical studies, particularly fermentation reactions because of its independence from fermentable substances. Several media containing Beef Extract Powder are recommended in standard methods for multiple application.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 10 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7228 Beef Extract Powder; and LabM Product: LABMC019 Beef Extract Powder |
| Package Weight | 26.60 lb |
Acid Hydrolysed Casein
Acid Hydrolysed Casein is for use in preparing microbiological culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans.
A soluble protein hydrolysate obtained by digesting casein with hot acid. It is almost free from growth factors, vitamins and antagonists, and these qualities make it suitable for use as a protein source in media for antibiotic and vitamin assays.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 10 kg |
| Previous Item Number | Product was previously known as LabM Product: MC007 Acid Hydrolysed Casein |
| Package Weight | 26.60 lb |
Casein Peptone Type I
Casein Peptone Type I is an enzymatic hydrolysate of casein for use in preparing microbiological culture media in a laboratory setting and is not intended for use in the diagnosis of disease or other conditions in humans.
Casein Peptone Type I is an enzymatic hydrolysate of casein, rich in peptones and amino acids (including tryptophane). This peptone can be utilized by most bacteria as a growth substrate.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 9191 Casein Peptone Type I; and LabM Product: MC005 Casein Peptone Type I |
| Package Weight | 14.00 lb |
Enzymatic Digest of Soy No. 2
Enzymatic Digest of Soy No. 2 is an enzymatic digest of soybean meal for use in preparing microbiological culture media in a laboratory setting. Enzymatic Digest of Soy No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.
Enzymatic Digest of Soy No. 2 is an enzymatic hydrolysate of soybean meal prepared under controlled conditions for use in microbiological procedures. Enzymatic Digest of Soy No. 2 is recommended for use in media for the cultivation of a large variety of organisms, including fungi and microbiological assay media. The nitrogen source in Enzymatic Digest of Soy No. 2 contains naturally occurring, high concentrations of vitamins and carbohydrates of soybean. Due to its high carbohydrate content, Enzymatic Digest of Soy No. 2 cannot be used for fermentation studies. This media ingredient produces clear solutions at culture media concentrations and neutral reactions after autoclaving.
Enzymatic Digest of Soy No. 2 minimizes Bovine Spongiform Encephalopathy (BSE) risk in vaccine production because of the plant origin of this product.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 10 kg |
| Grams per Liter | 40.00 |
| Liters per 500 g | 13.00 |
| Plates per 500 g | 680.00 |
| Previous Item Number | Product was previously known as Acumedia Product: 9213 Enzymatic Digest of Soy No.2 |
| Package Weight | 26.60 lb |
Tryptose
Tryptose is an enzymatic digest of protein for use in preparing microbiological culture media in a laboratory setting. Tryptose is not intended for use in the diagnosis of disease or other conditions in humans.
Tryptose was developed while studying growth requirements of Brucella spp. Huddleson found Tryptose media to be equal or superior to meat infusion media, providing uniformity for the cultivation and differentiation of fastidious organisms including Neisseria gonorrhoeae, Streptococcus milleri and Brucella spp.. Casman reported that a medium consisting of 2% Tryptose, along with other media ingredients, equaled fresh beef infusion base with respect to organism growth.
Tryptose is recommended in broth and agar formulations, and when used in media containing blood to permit hemolytic reactions. Tryptose is used in the preparation of Tryptose Broth (Product Code No. NCM0087). Tryptose media are recommended in standard methods for food testing.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7693 Tryptose; and LabM Product: LAB008 Tryptose |
| Package Weight | 14.00 lb |
Lecithin
Lecithin is used when preparing microbiology culture media in a laboratory setting, and is not intended for use in the diagnosis of disease or other conditions in humans. A deoiled, powdered soybean lecithin for use in microbiological culture media. A mixture of polar (phospho- and glyco-) lipids and a small amount of carbohydrates.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC041 Lecithin |
| Package Weight | 15.30 lb |
Glucose (Dextrose)
Glucose is a fermentable carbohydrate and is used when preparing microbiological culture media in a laboratory setting. Glucose is not intended for use in the diagnosis of disease or other conditions in humans.
Glucose is a fermentable carbohydrate which acts as an energy source in culture media.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC013 Glucose (Dextrose) |
| Package Weight | 15.30 lb |
Agar No. 2 Bacteriological
Agar No. 2 Bacteriological is a clear bacteriological agar with high gel strength compatible with all culture media. Agar No. 2 Bacteriological is not intended for use in the diagnosis of disease or other conditions in humans.
A bacteriological agar which gives a firm gel at working concentrations of 1.0 to 1.5% which is reasonably clear. This agar is recommended for all culture media except sensitivity testing media and those where absolute clarity is advantageous.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC006 Agar No 2 Bacteriological General Purpose |
| Package Weight | 15.30 lb |
Skim Milk Powder
Skim Milk Powder is used when preparing microbiology culture media in a laboratory setting. Milk Powder is not intended for use in the diagnosis of disease or other conditions in humans.
A bacteriological grade of thermophile free spray dried skim milk. Used in Milk Plate Count Agar NCM0119 and in media for diagnostic tests involving the digestion or coagulation of casein and the fermentation of lactose. Recommended working concentration 10%.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC027 Skimmed Milk Powder |
| Package Weight | 15.30 lb |
Skim Milk, Agglomerated NFDM
Skim Milk, Agglomerated NFDM is dehydrated skim milk for use in preparing microbiological culture media in a laboratory setting. Skim Milk, Agglomerated NFDM is not intended for use in the diagnosis of disease or other conditions in humans.
Skim Milk, Agglomerated NFDM is soluble, spray-dried skim milk. When prepared in a 10% solution, it is equivalent to fresh skim milk. Skim Milk is added to enhance growth of Mycobacterium tuberculosis and Corynebacterium diphtheria in culture media. Skim Milk, Agglomerated NFDM is used for differentiating organisms based on coagulation and proteolysis of casein in foods and dairy products. Indicators of pH, Litmus and Bromocresol Purple, are used with Skim Milk, Agglomerated NFDM to determine acid production. Methylene Blue and Resazurin may be used with Skim Milk, Agglomerated NFDM as oxidation-reduction indicators.
| Brand | Neogen® Culture Media |
| Analyte | General Purpose |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7352 Skim Milk, Agglomerated NFDM |
| Package Weight | 26.60 lb |
Enterobacteria Enrichment (EE) Broth Mossel
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Gram-negative bacteria | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 45.00 | ||||||||||||||
| Liters per 500 g | 11.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7603 EE Broth, Mossel; and LabM Products: LAB091 EE Broth – Enterobacteriaceae Enrichment and HP003 Enterobacteria Enrichment Broth – Mossel (USP/EP/JP) | ||||||||||||||
| Package Weight | 14.00 lb |
