Culture Media
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Phenylethanol Agar
Phenylethanol Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Phenylethanol Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Brewer and Lilley reported the addition of phenylethanol to a nutritive medium permitted growth of Gram-positive organisms, but markedly to completely inhibited growth of Gram-negative organisms. Phenylethanol Agar inhibits swarming of Proteus spp., and can be used to selectively isolate anaerobic bacteria from specimens with mixed flora. Phenylethanol Agar is specified for use in several reference methods.
| Brand | Neogen® Culture Media | ||||||||||||
| Analyte | Gram-positive cocci | ||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||
| Mass | 10 kg | ||||||||||||
| Formula |
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| Grams per Liter | 42.50 | ||||||||||||
| Liters per 500 g | 12.00 | ||||||||||||
| Plates per 500 g | 640.00 | ||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7147 Phenylethanol Agar | ||||||||||||
| Package Weight | 26.60 lb |
Columbia CNA Agar
Columbia CNA Agar is used with blood for the selective isolation of Gram-positive cocci in a laboratory setting. Columbia CNA Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Ellner et al. described Columbia CNA Agar as a variation of Columbia Blood Agar Base that is selective for Gram-positive cocci. Colistin and Nalidixic Acid are added to the formula, selecting for Gram-positive organisms and fungi by suppressing Gram-negative bacteria.
| Brand | Neogen® Culture Media | ||||||||||||||||||
| Analyte | Gram-positive cocci | ||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||
| Formula |
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| Grams per Liter | 43.00 | ||||||||||||||||||
| Liters per 500 g | 12.00 | ||||||||||||||||||
| Plates per 500 g | 630.00 | ||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7126 Columbia CNA Agar | ||||||||||||||||||
| Package Weight | 14.00 lb |
Casman Medium Base
Casman Medium Base is used with blood for the isolation of Haemophilus influenzae, Neisseria gonorrhoeae and other fastidious microorganisms in a laboratory setting. Casman Medium Base is not intended for use in the diagnosis of disease or other conditions in humans.
In 1947, Casman described an infusion-free medium enriched with 5% blood for fastidious microorganisms incubated anaerobically. This medium replaced formulas containing fresh meat infusion and heated blood. Casman adjusted the formula after experiments revealed nicotinamide disrupted the action of a blood enzyme that inactivates V factor (NAD). The concentration of nicotinamide was lowered to support growth of Neisseria spp.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||
| Analyte | Haemophilus influenzae, Neisseria gonorrhoeae | ||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||
| Mass | 10 kg | ||||||||||||||||||||||
| Formula |
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| Grams per Liter | 43.00 | ||||||||||||||||||||||
| Liters per 500 g | 12.00 | ||||||||||||||||||||||
| Plates per 500 g | 630.00 | ||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7123 Casman Medium Base | ||||||||||||||||||||||
| Package Weight | 26.60 lb |
APT Agar
APT Agar is used for the cultivation of heterofermentative lactobacilli in a laboratory setting. APT Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Evans and Niven investigated the cultivation of heterofermentative lactobacilli, causing the faded or green discoloration of cured meat products. Deibel, Evans, and Niven tested thiamine-requiring bacteria, specifically Lactobacillus viridescens. Their formulations led to the development of APT Agar.
Lactic acid bacteria, a group of acid-producing bacteria, include the genera Streptococcus, Leuconostoc, Pediococcus, and Lactobacillus. These organisms are widespread in nature, associated with bacterial spoilage of foods including dairy products, meat, and vegetables. APT Agar is used for cultivating heterofermentative lactic acid bacteria from food products.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||||
| Analyte | Heterofermentative lactobacilli | ||||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 58.00 | ||||||||||||||||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||||||||||||||||
| Plates per 500 g | 470.00 | ||||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7302 APT Agar | ||||||||||||||||||||||||||
| Package Weight | 14.00 lb |
m-HPC Agar
m-HPC Agar is used for the enumeration of heterotrophic organisms in water by the membrane filtration technique in a laboratory setting. m-HPC Agar is not intended for use in the diagnosis of disease or other conditions in humans.
m-HPC Agar, also known as m-Heterotrophic Plate Count Agar, was developed by Taylor and Geldreich in 1979. Monitoring the levels of heterotrophic bacteria, those microorganisms that can grow on minimal organic nutrients, is currently performed to indicate problems with treatment methods within distribution systems. There is evidence that heterotrophic bacteria are not hazardous to healthy individuals, but may be opportunistic pathogens capable of infecting individuals with impaired immune systems.
m-HPC Agar is used in standard methods for the detection of heterotrophic bacteria using the membrane filtration technique. The membrane filter technique is a microbiological method used in testing large volume samples. The concentration of larger samples on a membrane filter is a key benefit of the membrane filtration procedure. Several studies reported m-HPC Agar as a suitable alternate medium for standard total plate count agar.
| Brand | Neogen® Culture Media | ||||||||
| Analyte | Heterotrophic | ||||||||
| Platform | Dehydrated Culture Media | ||||||||
| Mass | 5 kg | ||||||||
| Formula |
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| Grams per Liter | 60.00 | ||||||||
| Liters per 500 g | 8.00 | ||||||||
| Plates per 500 g | 450.00 | ||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7690 m-HPC Agar | ||||||||
| Package Weight | 15.30 lb |
R2A Agar
R2A Agar is used for the enumeration and cultivation of bacteria from potable water in a laboratory setting. R2A Agar is not intended for use in the diagnosis of disease or other conditions in humans. R2A Agar was developed by Reasoner and Geldreich for bacterial plate counts of treated potable water. R2A Agar is a low nutrient medium, and in combination with a lower incubation temperature and longer incubation time, stimulates the growth of stressed and chlorine-tolerant bacteria. Nutritionally rich media support the growth of fast-growing bacteria, and may suppress slow growing or stressed bacteria found in treated water. When compared with Tryptone Glucose Yeast Extract Agar or Plate Count Agar (Standard Methods Agar), R2A Agar reported improved recovery of stress and chlorine-tolerant bacteria from drinking water systems. R2A Agar is recommended in standard methods for pour plate, spread plate, and membrane filter methods for heterotrophic plate counts.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||
| Analyte | Heterotrophs | ||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||
| Formula |
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| Grams per Liter | 18.20 | ||||||||||||||||||||||
| Liters per 500 g | 28.00 | ||||||||||||||||||||||
| Plates per 500 g | 1,520.00 | ||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7390 R2A Agar; and LabM Product: LAB163 R2A Medium | ||||||||||||||||||||||
| Package Weight | 14.00 lb |
Raka-Ray No. 3 Agar
Raka-Ray No.3 Agar is for the detection of lactic acid bacteria in beer and for monitoring in-process beer quality, and is not intended for use in the diagnosis of disease or other conditions in humans. Raka-Ray No.3 Agar is for the detection of lactic acid bacteria in beer and for monitoring in-process beer quality. It is recommended for this application by the European Brewing Convention (EBC) and the American Society of Brewing Chemists (ASBC). Contamination of beer and the beer making process by members of the lactobacilli family results in spoilage, primarily through their production of metabolic products which are detrimental to the flavor of the final product. Detection of these organisms is complicated by their diverse nutritional and environmental requirements. A number of different formulations have been described for the isolation of lactic acid bacteria in brewing products and processes. Raka-Ray Agar was developed by the addition of various growth promoting compounds to Universal Beer Agar. This work led to the recognition that the addition of sorbitan mono-oleate, liver extract and N-acetylglucosamine produced superior growth when compared to the standard Universal Beer Agar formulation. Further investigations provided the basis for the final formula of Raka-Ray No. 3 Medium in which fructose is an essential carbohydrate source for Lactobacillus fructivorans. Maltose is present to allow the growth of lactobacilli which cannot utilize glucose. The media can be made selective against yeasts by the addition of 7mg/l cycloheximide (Actidione®) and against Gram-negative bacteria by the addition of 3g/L 2-phenylethanol.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||||||||||
| Analyte | Lactic Acid Bacteria | ||||||||||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 77.10 | ||||||||||||||||||||||||||||||||
| Liters per 500 g | 6.00 | ||||||||||||||||||||||||||||||||
| Plates per 500 g | 350.00 | ||||||||||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as LabM Product: LAB198 Raka-Ray No.3 Agar | ||||||||||||||||||||||||||||||||
| Package Weight | 15.30 lb |
Tomato Juice Agar
Tomato Juice Agar is used for the cultivation of lactobacilli in a laboratory setting. Tomato Juice Agar is not intended for use in the diagnosis of disease or other conditions in humans.
In 1925, Mickle and Breed reported the use of tomato juice in culture media used for cultivating lactobacilli. Kulp investigated tomato juice on bacterial development and found growth of L. acidophilus was enhanced. Tomato Juice Agar, prepared according to Kulp and White’s modification, is especially useful in cultivating L. acidophilus from foodstuffs.
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Lactobacillus spp. | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 500 g | ||||||||||
| Formula |
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| Grams per Liter | 51.00 | ||||||||||
| Liters per 500 g | 10.00 | ||||||||||
| Plates per 500 g | 530.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7349 Tomato Juice Agar | ||||||||||
| Package Weight | 15.30 lb |
Lactobacillus Selective Agar Base
Lactobacillus Selective Agar Base is used for the isolation and enumeration of lactobacilli in a laboratory setting. Lactobacillus Selective Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
Lactobacillus Selective Agar Base was developed by Rogosa, Mitchell, and Wiseman. This medium is used for isolation, enumeration, and identification of lactobacilli in foods. The low pH and high acetate concentrations effectively suppress other bacterial flora allowing lactobacilli to flourish.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||
| Analyte | Lactobacillus spp. | ||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 84.00 | ||||||||||||||||||||||||
| Liters per 500 g | 6.00 | ||||||||||||||||||||||||
| Plates per 500 g | 320.00 | ||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7234 Lactobacillus Selective Agar Base | ||||||||||||||||||||||||
| Package Weight | 15.30 lb |
Lactobacilli MRS Agar
Lactobacilli MRS Agar is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans. MRS Agar is a medium for the cultivation and enumeration of Lactobacillus spp. Originally developed in 1960 by de Man, Rogosa & Sharpe, the medium is suitable for most lactic acid bacteria and is intended as a substitute for Tomato Juice Agar. When acidified to pH 5.4 MRS Agar can be used to enumerate Lactobacillus bulgaricus in yogurts. Nutrition is provided by a mixture of carefully selected peptones, glucose, beef & yeast extracts while Tween® 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli while inhibiting a number of other microorganism groups. Occasionally, sterilization of this medium at 121°C for 15 minutes, in some autoclaves, may cause the pH to fall outside of the specified pH limits 6.4 +/- 0.2. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||
| Analyte | Lactobacillus spp. | ||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 70.00 | ||||||||||||||||||||||||
| Liters per 500 g | 7.00 | ||||||||||||||||||||||||
| Plates per 500 g | 390.00 | ||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7543 Lactobacilli MRS Agar; and LabM Product: LAB093 M.R.S Agar – de Man, Rogosa and Sharpe Agar | ||||||||||||||||||||||||
| Package Weight | 14.00 lb |
Lactobacilli MRS Broth
Lactobacilli MRS Broth is used for the cultivation of lactobacilli and is not intended for use in the diagnosis of disease or other conditions in humans.
Lactobacilli MRS broth was originally developed in 1960 by de Man, Rogosa & Sharpe, the medium can be used for confirmatory tests on organisms isolated on MRS Agar. The medium can also be used for enumeration by the Miles and Misra technique.
Nutrition is provided by a mixture of carefully selected peptones, glucose, beef and yeast extracts while Tween® 80, magnesium and manganese sulfates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate.
Occasionally, sterilization of this medium at 121°C for 15 minutes, in some autoclaves, may cause the pH to fall outside of the specified pH limits 6.4 +/- 0.2. In these rare cases, adjustment of the medium using acetic acid or sodium hydroxide is recommended.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||
| Analyte | Lactobacillus spp. | ||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||
| Formula |
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| Grams per Liter | 55.00 | ||||||||||||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7406 Lactobacilli MRS Broth ; and LabM Product: LAB094 M.R.S. Broth | ||||||||||||||||||||||
| Package Weight | 14.00 lb |
Enterobacteria Enrichment (EE) Broth Mossel
Enterobacteria Enrichment (EE) Broth Mossel is used for the cultivation and enrichment of Enterobacteriaceae in food. Enterobacteria Enrichment (EE) Broth Mossel, conforms to Harmonized USP/EP/JP Requirements and is not intended for use in the diagnosis of disease or other conditions in humans. Enterobacteria Enrichment Broth Mossel was developed by Mossel, Visser, and Cornelissen to facilitate the growth of Enterobacteriaceae. This medium contains dextrose to enhance the growth of E. coli and Salmonella spp., particularly in food samples. Nuisance organisms are suppressed by the addition of Ox Bile and Brilliant Green. Enterobacteria Enrichment Broth Mossel is used as an enrichment broth, providing a rich environment for the recovery of damaged or injured cells. Enterobacteriaceae organisms can be injured in food-processing procedures, including exposure to low temperature, sub-marginal heat, drying, radiation, preservatives, or sanitizers. The enumeration of Enterobacteriaceae is an important measure of the sanitary condition of food. EE Broth, Mossel complies with the specifications of the Eiprodukte-Verodnung (German Egg Product Regulations) and conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Gram-negative bacteria | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 45.00 | ||||||||||||||
| Liters per 500 g | 11.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7603 EE Broth, Mossel; and LabM Products: LAB091 EE Broth – Enterobacteriaceae Enrichment and HP003 Enterobacteria Enrichment Broth – Mossel (USP/EP/JP) | ||||||||||||||
| Package Weight | 14.00 lb |
MRS Agar
MRS Agar is a medium for the enumeration of mesophilic lactic acid bacteria according to ISO 15214:1998, and is not intended for use in the diagnosis of disease or other conditions in humans.
MRS Agar was originally developed in 1960 by de Man, Rogosa & Sharpe for the cultivation and enumeration of Lactobacillus spp. from various sources and is intended as a substitute for Tomato Juice Agar. This original formulation has been adapted and adjusted to pH 5.7 according to ISO 15214:1998.
Nutrition is provided by enzymatic digest of casein, glucose, meat & yeast extracts whilst polyoxyethylenesorbitan monooleate, magnesium and manganese sulphates act as growth stimulants. Selectivity against streptococci & molds is provided by ammonium citrate and sodium acetate. Used at low pH, ammonium citrate allows growth of lactobacilli whilst inhibiting a number of other organism groups.
Occasionally, sterilization of this medium at 121°C can cause the pH to fall outside of the specified pH limits 5.7 +/- 0.1. In these rare cases adjustment of the medium using acetic acid or sodium hydroxide is recommended.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||
| Analyte | Lactobacillus spp. | ||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 70.00 | ||||||||||||||||||||||||
| Liters per 500 g | 7.00 | ||||||||||||||||||||||||
| Plates per 500 g | 390.00 | ||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as LabM Product: LAB223 MRS Agar (ISO) | ||||||||||||||||||||||||
| Package Weight | 15.30 lb |
Ceftazidime (20mg/L)
A selective culture media supplement designed for the selection and isolation of Listeria spp.
| Brand | Neogen® Culture Media |
| Analyte | Listeria |
| Platform | Dehydrated Culture Media |
| Formula | Ceftazidime – 20 mg/L |
| Preparation | Reconstitute using sterile deionized/RO water |
| Previous Item Number | Product was previously known as Abtek product: #07-IS/LM6 Ceftazadime |
| Storage Conditions | 2-8°C |
| Package Dimensions | 4.60 in x 2.00 in x 2.10 in |
| Package Weight | 1.10 lb |
Half Fraser (Demi-Fraser) Broth
Ready-to-reconstitute media bags for Half Fraser Broth contain exactly the correct amount of dehydrated culture media and supplements to prepare 20 L of enrichment broth. The dehydrated culture media contained in each bag has been sterilized via irradiation and needs reconstituting with sterile deionized/RO water.
Half Fraser (Demi-Fraser) Broth (ISO) is a selective medium used for the primary enrichment of Listeria monocytogenes and Listeria spp., as described in ISO 11290-1:2017. Half Fraser (Demi-Fraser) Broth (ISO) was developed as a modification of UVM medium (NCM0012) and is based on the formula as described by Fraser and Sperber. Listeria spp. hydrolyze the esculin to esculetin, which reacts with the ferric ammonium citrate (NCM4009), resulting in a black precipitate and a visible positive reaction.
Lithium chloride suppresses the growth of Enterococci in the medium, as Enterococci can also hydrolyze esculin. Enzymatic digestion of animal tissues, casein, meat extract, and yeast extract provides essential vitamins, minerals, amino acids, nitrogen, and carbon, while sodium chloride maintains the osmotic balance. The selective components acriflavine and nalidixic acid are blended into the base powder and inhibit the growth of Escherichia coli. Ferric ammonium citrate is added to the tempered broth after sterilization.
According to ISO 11290-1:2017, the test portion is inoculated into the primary enrichment medium Half Fraser (Demi-Fraser) Broth (ISO), followed by secondary enrichment in Fraser Broth (ISO), and then sub-cultured onto Harlequin® Listeria Chromogenic Agar according to Ottaviani and Agosti (NCM1004). The medium conforms to the performance and formulation requirements of ISO 11290-1:2017.
| Brand | Neogen® Culture Media |
| Analyte | Listeria monocytogenes, Listeria spp. |
| Platform | Dehydrated Culture Media |
| Result Type | Presence/Absence |
| Previous Item Number | Product was previously known as Lab M® product # MPB004 |
| Storage Conditions | 10-25 °C away from direct sunlight |
| Quantity per Package | 5 |
| Package Weight | 14.50 lb |
Oxford Listeria Agar
Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage and river water. Oxford Listeria Agar is prepared according to the formulation of Curtis et al. Listeria spp. grow over a pH range of 5.0 – 9.6 and survive in food products with pH levels outside these parameters.
| Brand | Neogen® Culture Media | ||||||||||||
| Analyte | Listeria monocytogenes | ||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||
| Mass | 5 kg | ||||||||||||
| Formula |
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| Grams per Liter | 57.50 | ||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||
| Plates per 500 g | 470.00 | ||||||||||||
| Preparation |
*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions. | ||||||||||||
| Previous Item Number | Product was previously known as LabM Products: LAB122 Listeria Isolation Medium (Oxford) & LAB206 Listeria Isolation Media | ||||||||||||
| Package Weight | 14.00 lb |
Palcam Agar Base
Palcam Agar Base is used with supplements as a selective and differential medium for the detection and isolation of Listeria monocytogenes from food and environmental samples. Palcam Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
Palcam Agar was developed by Van Netten et al in 1989 as an improved selective differential medium for the isolation of Listeria monocytogenes from food and environmental specimens. Improved selectivity is achieved by the combination of antibiotic supplements and microaerobic incubation. The double indicator system of esculin hydrolysis and mannitol fermentation aids differentiation of Listeria spp from enterococci and staphylococci which can be confused with Listeria spp on other types of culture media. Palcam Agar is listed within Annex E of ISO 11290-1:2017 as a suitable second selective agar.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||
| Analyte | Listeria monocytogenes | ||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 71.00 | ||||||||||||||||||||||||
| Liters per 500 g | 7.00 | ||||||||||||||||||||||||
| Plates per 500 g | 380.00 | ||||||||||||||||||||||||
| Preparation |
*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions. | ||||||||||||||||||||||||
| Previous Item Number | Product was previously known as Acumedia Product: 7669 PALCAM Agar; and LabM Product: LAB148 PALCAM Agar Base | ||||||||||||||||||||||||
| Package Weight | 14.00 lb |
LESS Plus Medium Irradiated
LESS Plus Medium Irradiated is a proprietary broth intended to selectively enrich Listeria monocytogenes and Listeria spp. in a broad range of food and environmental sample as part of a rapid method. LESS Plus medium is available exclusively for use as part of Neogen’s Listeria testing solutions.
| Brand | Neogen® Culture Media |
| Analyte | Listeria monocytogenes, Listeria spp. |
| Platform | Dehydrated Culture Media, One Broth One Plate |
| Result Type | Qualitative |
| Approvals | This product forms part of Neogen’s innovative One Broth One Plate for Listeria (OBOP-L) workflow that has been validated according to ISO 16140-2 and certified by AFNOR. |
| Mass | 5 kg |
| Grams per Liter | 44.00 |
| Liters per 500 g | 11.00 |
| Storage Conditions | 2-30 °C away from direct sunlight |
| Package Weight | 15.30 lb |
Half Fraser (Demi-Fraser) Broth (ISO)
Half Fraser (Demi-Fraser) Broth is used with ferric ammonium citrate for the selective enrichment of Listeria species and is not intended for use in the diagnosis of disease or other conditions in humans. Primary enrichment broth for the isolation of Listeria spp. formulated according to ISO 11290. The selective components acriflavine and nalidixic acid are blended into the base powder and the ferric ammonium citrate is added to the tempered broth after sterilization. Half Fraser (Demi-Fraser) Broth, NCM0001 was developed to give improved results for the isolation of Listeria spp. with both traditional and ELISA methods. As with the sister product, Fraser Broth, NCM0050 the selective components acriflavine and nalidixic acid are blended into the base powder and the Ferric Ammonium Citrate is added to the tempered broth after autoclaving. This format has been shown to give improved selectivity with pure cultures and food samples. Furthermore, more stable ELISA results are seen, resulting in fewer false positive results when the ferric ammonium citrate is omitted from the complete media. Developed as a modification of UVM medium (NCM0012) and made according to ISO 11290, Fraser Broth is a secondary enrichment broth for the isolation of Listeria spp. and is similar to Palcam Broth (NCM0049) in that it contains esculin to indicate the presence of a potential Listeria isolate. It also contains lithium chloride in an attempt to suppress the growth of Enterococci in the medium (as does Palcam). Listeria spp. hydrolyze the esculin to form esculetin, which reacts with the ferric ammonium citrate in resulting in a black precipitate and a visible positive reaction. However, Enterococci can also perform this reaction so further plating is required onto an isolation medium such as Harlequin® Listeria Chromogenic Agar (NCM1004).
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||
| Formula |
*Equivalent to Disodium Hydrogen Phosphate Dihydrate 12.0 g/L | ||||||||||||||||||||||||
| Grams per Liter | 55.00 | ||||||||||||||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||||||||||||||
| Preparation |
*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions. | ||||||||||||||||||||||||
| Previous Item Number | Product was previously known as Acumedia Product: 7656 Demi-Fraser Broth Base; and LabM Product: LAB211 Half Fraser Broth PLUS | ||||||||||||||||||||||||
| Package Weight | 14.00 lb |
Listeria Enrichment Broth
Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.
Listeria monocytogenes, first described in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.
Listeria Enrichment Broth is based on the formula developed by Lovett et al. in which Tryptic Soy Broth is supplemented with Yeast Extract for optimum growth. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.
| Brand | Neogen® Culture Media | ||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||
| Formula |
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| Grams per Liter | 36.10 | ||||||||||||||||||||
| Liters per 500 g | 14.00 | ||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7398 Listeria Enrichment Broth | ||||||||||||||||||||
| Package Weight | 14.00 lb |
Fraser Broth
Fraser Broth is a selective medium used for the secondary enrichment of Listeria monocytogenes and Listeria spp., as described in ISO 11290-1:2017. Fraser Broth is not intended for use in the diagnosis of disease or other conditions in humans.
Fraser Broth was developed as a modification of UVM medium (NCM0012) and is based on the formula as described by Fraser and Sperber. Listeria spp. hydrolyze the esculin to esculetin which reacts with the ferric ammonium citrate (X211 or 7984) resulting in a black precipitate and a visible positive reaction.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 55.00 | ||||||||||||||||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||||||||||||||||
| Preparation |
*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions. | ||||||||||||||||||||||||||
| Previous Item Number | Product was previously known as Acumedia Product: 7502 Fraser Broth Base; and LabM Product: LAB212 Fraser Broth PLUS | ||||||||||||||||||||||||||
| Package Weight | 14.00 lb | ||||||||||||||||||||||||||
Buffered Listeria Enrichment Broth
Buffered Listeria Enrichment Broth is used for selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans.
Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. Evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.
Buffered Listeria Enrichment Broth, a modification of the formula by Lovett et al., was developed after subsequent work concluded that enrichment properties can be improved by increasing the buffering capacity of the medium with the addition of Disodium Phosphate. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 47.00 | ||||||||||||||||||||||||
| Liters per 500 g | 11.00 | ||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7579 Buffered Listeria Enrichment Broth; and LabM Product: LAB579 Buffered Listeria Enrichment Broth | ||||||||||||||||||||||||
| Package Weight | 14.00 lb |
LEE Broth
Listeria Express Enrichment Broth (LEE Broth) is a selective enrichment broth for the detection of Listeria from foods and is not intended for use in the diagnosis of disease or other conditions in humans. Developed to give improved growth rates of Listeria over traditional selective enrichment media, LEE Broth enhances the expression of target antigens for most commercially available immunological test kits/methods whilst maintaining adequate suppression of potential non-target organisms. Selective components are blended into the powder, removing the requirement for supplementation. Compared with more traditional methods, immunological tests, such as ELISA, require relatively high levels of target organisms to achieve a reliable positive result. LEE Broth has been specifically designed to stimulate growth from low numbers to the required high levels within a 24 hour period. Therefore, LEE Broth offers an excellent choice for laboratories employing immunological test methods for detection of Listeria in food products. LEE Broth can be used as an enrichment broth prior to plating on selective media such as Harlequin® Listeria Chromogenic Agar ISO (NCM1004 / NCM3001); as secondary selective enrichment medium following primary enrichment in, for example, Half Fraser Broth (NCM0001 or NCM0066); or as the enrichment step of a rapid method e.g. ELISA, lateral flow device, PCR.
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Listeria spp. | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 500 g | ||||||||||
| Formula |
| ||||||||||
| Grams per Liter | 46.20 | ||||||||||
| Liters per 500 g | 11.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as LabM Product: LAB589 LEE Broth – Listeria Express Enrichment Broth | ||||||||||
| Package Weight | 1.35 lb |
Harlequin® Listeria Chromogenic Agar (Ottaviani & Agosti) (LCA)
Harlequin® Listeria Chromogenic Agar (according to the formulation of Ottaviani and Agosti) is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials as described in ISO 11290-1:2017. This medium is not intended to diagnose diseases or other conditions in humans.
Lithium chloride in the base medium and supplementary antimicrobial compounds ceftazidime, polymyxin, nalidixic acid, and cycloheximide provide the medium’s selectivity. Chromogenic activity is a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. and a few strains of Enterococci and Bacilli.
The specific differential activity of this agar is obtained with a proprietary lecithin substrate for the detection of the phospholipase enzyme present in the L. monocytogenes colonies growing on this media. This enzyme activity will result in a halo of precipitation surrounding the target colonies.
Combining both the chromogenic and phospholipase enzyme reactions makes it possible to differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo).
LCA Agar forms part of Neogen’s innovative One Broth One Plate Listeria (OBOP-L) workflow that has been validated according to ISO 16140-2 and certified by AFNOR.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||||||||
| Platform | Dehydrated Culture Media, One Broth One Plate | ||||||||||||||||||||||||||
| Result Type | Qualitative | ||||||||||||||||||||||||||
| Approvals | This product forms part of Neogen’s innovative One Broth One Plate Listeria (OBOP-L) workflow that has been validated according to ISO 16140-2 and certified by AFNOR. | ||||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 70.50 | ||||||||||||||||||||||||||
| Liters per 500 g | 7.00 | ||||||||||||||||||||||||||
| Plates per 500 g | 390.00 | ||||||||||||||||||||||||||
| Preparation |
*Larger vials may be available. Please see the appropriate supplement data sheet for availability and preparation instructions. | ||||||||||||||||||||||||||
| Previous Item Number | Product was previously known as LabM Product: HAL010 Harlequin® Listeria Chromogenic Agar | ||||||||||||||||||||||||||
| Storage Conditions | 2-8 °C away from direct sunlight | ||||||||||||||||||||||||||
| Package Weight | 15.30 lb |
Buffered Listeria Enrichment Broth Base
Buffered Listeria Enrichment Broth Base is used with supplements for selective enrichment of Listeria spp. in foods and is not intended for use in the diagnosis of human disease.
Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey, frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate.
Buffered Listeria Enrichment Broth, a modification of the formula by Lovett et al. was developed after research concluded that enrichment properties can be improved by increasing the buffering capacity of the medium with the addition of disodium phosphate. BLEB Base is based upon the FDA/BAM recommendations where the medium is supplemented with selective agents after an initial four hour, non-selective, pre-enrichment.
| Brand | Neogen® Culture Media | ||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||
| Formula |
| ||||||||||||||||||
| Grams per Liter | 47.00 | ||||||||||||||||||
| Liters per 500 g | 11.00 | ||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7675 Buffered Listeria Enrichment Broth Base (BLEB); and LabM Product: LAB139/LAB582 Buffered Listeria Broth | ||||||||||||||||||
| Package Weight | 14.00 lb |
UVM Broth
UVM Broth is used for the selective enrichment of Listeria spp and is not intended for use in the diagnosis of disease or other conditions in humans. UVM Broth is a modification of the formula described by Donnelly and Baigent. This formula is used for the selective enrichment of Listeria spp. from food samples. Listeria spp. grow over a pH range of 5.0 – 9.6 and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation. Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Beef Extract, and Yeast Extract provide nitrogen, vitamins, and minerals in UVM Broth. The Phosphates are the buffering agents, Sodium Chloride maintains osmotic balance. Nalidixic Acid inhibits growth of Gram-negative organisms. Acriflavin inhibits Gram-positive bacteria. Esculin is hydrolyzed by Listeria spp. The high salt tolerance of Listeria is used as a means to inhibit growth of enterococci.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||
| Formula |
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| Grams per Liter | 52.00 | ||||||||||||||||||||||
| Liters per 500 g | 10.00 | ||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7409 UVM Modified Listeria Enrichment Broth; and LabM Product: LAB155 UVM Broth Base | ||||||||||||||||||||||
| Package Weight | 14.00 lb |
PALCAM Broth
PALCAM Broth is used with supplements for the selective enrichment of Listeria spp. from foods and environmental samples in a laboratory setting. PALCAM Broth is not intended for use in the diagnosis of disease or other conditions in humans.
Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in food industries. Evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. PALCAM Broth is based on the formulation of Van Netten et al. while he was investigating the isolation of Listeria spp. from food samples. PALCAM Broth has been used for testing Listeria from raw milk.
| Brand | Neogen® Culture Media | ||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||
| Formula |
| ||||||||||||||||||||
| Grams per Liter | 47.00 | ||||||||||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||||||||||
| Preparation |
*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions. | ||||||||||||||||||||
| Previous Item Number | Product was previously known as Acumedia Product: 7370 PALCAM Broth; and LabM Product: LAB144 PALCAM Broth | ||||||||||||||||||||
| Package Weight | 14.00 lb |
Blood Agar Base
Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. An inexpensive, general purpose agar base which, with the addition of 5-7% sterile blood, can be used to cultivate a wide range of microorganisms of indicative significance. Typical hemolysis patterns are obtained with this medium.
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Campylobacter spp., Clostridium, Listeria spp., Staphylococci, Streptococci | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 5 kg | ||||||||||
| Formula |
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| Grams per Liter | 37.00 | ||||||||||
| Liters per 500 g | 14.00 | ||||||||||
| Plates per 500 g | 740.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7268 Blood Agar Base, Improved; and LabM Product: LAB028 Blood Agar Base | ||||||||||
| Package Weight | 15.30 lb |
Fraser Broth Base
Fraser Broth Base is used with Acriflavin, Nalidixic Acid, and Ferric Ammonium Citrate for the selective enrichment of Listeria spp. Fraser Broth Base is not intended for use in the diagnosis of disease or other conditions in humans.
Formulated according to MLG Appendix 1.09 (for the ISO 11290 formulation please use NCM0001). Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem in public health and food industries. This organism has the ability to cause human illness and death, particularly in immunocompromised individuals and pregnant women. Epidemiological evidence from outbreaks of listeriosis indicated the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese and pate.
Half Fraser (Demi Fraser) Broth Base is based on the formulation of Fraser and Sperber. This medium is used in rapid detection of Listeria from food and environmental samples. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria spp. is based on successful isolation of the organism, biochemical characterization, and serological confirmation.
| Brand | Neogen® Culture Media | ||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||
| Formula |
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| Grams per Liter | 57.40 | ||||||||||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||||||||||
| Preparation |
*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions. | ||||||||||||||||||||
| Previous Item Number | Product was previously known as Acumedia Product: 7626 Fraser Broth Base | ||||||||||||||||||||
| Package Weight | 14.00 lb |
Dextrose Tryptone Broth
Dextrose Tryptone Broth is used for cultivation of mesophilic or thermophilic spoilage microorganisms from food in a laboratory setting. Dextrose Tryptone Broth is not intended for use in the diagnosis of disease or other conditions in humans.
In the 1930’s, the National Canners Association specified use of Dextrose Tryptone Agar for isolating “flat sour” organisms from food products. “Flat sour” spoilage of canned foods is caused by Bacillus coagulans (Bacillus thermoacidurans). Bacterial growth results in a 0.3 – 0.5 drop in pH, while ends of the can remain flat. B. coagulans is a soil microorganism and found in canned tomato products and dairy products. Conditions favorable for multiplication of the bacterium can result in spoilage of the food product.
Dextrose Tryptone Broth is similar to Dextrose Tryptone Agar, except the concentration of Tryptone and Dextrose has been doubled and Agar is removed. The American Public Health Association and Baumgartner and Herson recommended this formulation for the bacteriological examination of low and medium-acid canned foods (pH 4.5 and above).
| Brand | Neogen® Culture Media | ||||||||
| Analyte | Mesophilic or thermophilic spoilage | ||||||||
| Platform | Dehydrated Culture Media | ||||||||
| Mass | 5 kg | ||||||||
| Formula |
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| Grams per Liter | 30.00 | ||||||||
| Liters per 500 g | 17.00 | ||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7338 Dextrose Tryptone Broth | ||||||||
| Package Weight | 14.00 lb |
NFL Aseptic Validation Medium
National Food Laboratory Aseptic Validation Medium is used for the cultivation of mesophilic or thermophilic spoilage microorganisms in food in a laboratory setting. National Food Laboratory Aseptic Validation Medium is not intended for use in the diagnosis of disease or other conditions in humans.
Foods may spoil either due to biological or chemical reasons. Spore-forming bacteria, e.g., Clostridium and Bacillus, are important because of their heat resistant (thermophilic) nature. In addition, there are other microorganisms which are not heat resistant (mesophilic) but enter through the leakage of the container during cooling.
Examples of spoilage type thermophilic microorganisms are flat-sour and sulfide spoilage and can be found in corn, peas and spinach. Mesophilic microorganisms include putrefactive anaerobes, butyric anaerobes, and aciduric flat-sour, and can be found in tomatoes and peas.
In the 1930’s, the National Canners Association specified use of Dextrose Tryptone Agar for isolating “flat sour” organisms from food products.
National Food Laboratory Aseptic Validation Medium is a general purpose medium for the recovery of aerobic and facultative organisms used in the validation and commissioning of aseptic filling systems. This medium is ideally suited for the spore-forming organisms used in the validation of steam, hydrogen peroxide, and peracetic acid sterilization systems.
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Mesophilic or thermophilic spoilage | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 5 kg | ||||||||||
| Formula |
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| Grams per Liter | 16.00 | ||||||||||
| Liters per 500 g | 31.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7737 National Food Laboratory Aseptic Validation Medium | ||||||||||
| Package Weight | 14.00 lb |
HC Agar Base
HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
Methods for isolating molds from cosmetic products require incubation for 5 – 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||
| Analyte | Molds | ||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||
| Mass | 500 g | ||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 54.50 | ||||||||||||||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||||||||||||||
| Plates per 500 g | 500.00 | ||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7520 HC Agar Base | ||||||||||||||||||||||||
| Package Weight | 1.35 lb |
HC Agar BaseMiddlebrook 7H11 Agar
Middlebrook 7H11 Agar is used with glycerol and OADC Enrichment for the cultivation of Mycobacterium spp. in a laboratory setting. Middlebrook 7H11 Agar is not intended for use in the diagnosis of disease or other conditions in humans.
There are two types of solid culture media for the primary isolation of mycobacteria, coagulated egg as a base (Lowenstein formulations) and an agar base (Middlebrook formulations). The use of agar-based media for primary isolation of mycobacteria have the following significant advantages:
- Agar-based media do not usually liquefy in the presence of contaminating proteolytic organisms.
- Agar-based media retain exact concentrations of added drugs because the medium is solidified with agar rather than by inspissation of the egg. There is less drug inactivation when egg ingredients are absent.
Middlebrook 7H11 Agar is a modification of Middlebrook 7H10 Agar Special as recommended by Cohn, Waggoner, and McClately. Cohn et al. added an enzymatic digest of casein and found organism growth was stimulated for fastidious strains of Mycobacterium tuberculosis.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||||||||
| Analyte | Mycobacterium | ||||||||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 19.00 | ||||||||||||||||||||||||||||||
| Liters per 500 g | 26.00 | ||||||||||||||||||||||||||||||
| Plates per 500 g | 1,440.00 | ||||||||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7244 Middlebrook 7H11 Agar | ||||||||||||||||||||||||||||||
| Package Weight | 14.00 lb |
Lowenstein-Jensen Medium
Lowenstein – Jensen Medium is used with fresh egg and glycerol for the isolation and differentiation of Mycobacterium spp. in a laboratory setting. Lowenstein – Jensen Medium is not intended for use in the diagnosis of disease or other conditions in humans.
The use of egg-based media for primary isolation of mycobacteria have two significant advantages. First, egg-based media support a wide variety of mycobacteria. Second, growth of mycobacteria on egg media can be used for niacin testing. Liquefaction of Lowenstein-Jensen Medium can occur if contaminated with proteolytic organisms.
Lowenstein-Jensen Medium is a modification of Lowenstein Medium, modified by Jensen. Jensen modified the medium by alternating the citrate and phosphate contents, eliminating congo red dye, and increasing malachite green concentration. Lowenstein-Jensen Medium is commonly used in the clinical laboratory to isolate acid-fast organisms from sterile and nonsterile sources.
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Mycobacterium spp. | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 37.30 | ||||||||||||||
| Liters per 500 g | 13.00 | ||||||||||||||
| Plates per 500 g | 730.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7245 Lowenstein-Jensen Medium | ||||||||||||||
| Package Weight | 15.30 lb |
PPLO Broth Base without Crystal Violet
PPLO Broth Base without Crystal Violet is used with enrichments for the isolation and cultivation of Mycoplasma spp. in a laboratory setting. PPLO Broth without Crystal Violet is not intended for the use in the diagnosis of disease or other conditions in humans.
PPLO Broth Base without Crystal Violet is prepared according to the formula described by Morton and Lecce. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as “pleuropneumonia-like organism” or PPLO. Mycoplasmas belong to the class of Mollicutes “soft skin”, which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 μm.
| Brand | Neogen® Culture Media | ||||||||
| Analyte | Mycoplasma spp. | ||||||||
| Platform | Dehydrated Culture Media | ||||||||
| Mass | 10 kg | ||||||||
| Formula |
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| Grams per Liter | 21.00 | ||||||||
| Liters per 500 g | 24.00 | ||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 0113 PPLO Broth Base without Crystal Violet; and LabM Product: LAB229 PPLO Broth Base without Crystal Violet | ||||||||
| Package Weight | 26.60 lb |
