Culture Media
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PALCAM Broth
PALCAM Broth is used with supplements for the selective enrichment of Listeria spp. from foods and environmental samples in a laboratory setting. PALCAM Broth is not intended for use in the diagnosis of disease or other conditions in humans.
Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in food industries. Evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. PALCAM Broth is based on the formulation of Van Netten et al. while he was investigating the isolation of Listeria spp. from food samples. PALCAM Broth has been used for testing Listeria from raw milk.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0049B | |
| 10 kg | NCM0049C | |
| 500 g | NCM0049A |
| Brand | Neogen® Culture Media | ||||||||||||||||||||
| Analyte | Listeria spp. | ||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||
| Formula |
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| Grams per Liter | 47.00 | ||||||||||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||||||||||
| Preparation |
*Larger vials may be available. Please see appropriate supplement data sheet for availability and preparation instructions. | ||||||||||||||||||||
| Previous Item Number | Product was previously known as Acumedia Product: 7370 PALCAM Broth; and LabM Product: LAB144 PALCAM Broth | ||||||||||||||||||||
| Package Weight | 14.00 lb |
Middlebrook 7H11 Agar
Middlebrook 7H11 Agar is used with glycerol and OADC Enrichment for the cultivation of Mycobacterium spp. in a laboratory setting. Middlebrook 7H11 Agar is not intended for use in the diagnosis of disease or other conditions in humans.
There are two types of solid culture media for the primary isolation of mycobacteria, coagulated egg as a base (Lowenstein formulations) and an agar base (Middlebrook formulations). The use of agar-based media for primary isolation of mycobacteria have the following significant advantages:
- Agar-based media do not usually liquefy in the presence of contaminating proteolytic organisms.
- Agar-based media retain exact concentrations of added drugs because the medium is solidified with agar rather than by inspissation of the egg. There is less drug inactivation when egg ingredients are absent.
Middlebrook 7H11 Agar is a modification of Middlebrook 7H10 Agar Special as recommended by Cohn, Waggoner, and McClately. Cohn et al. added an enzymatic digest of casein and found organism growth was stimulated for fastidious strains of Mycobacterium tuberculosis.
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||||||||
| Analyte | Mycobacterium | ||||||||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 19.00 | ||||||||||||||||||||||||||||||
| Liters per 500 g | 26.00 | ||||||||||||||||||||||||||||||
| Plates per 500 g | 1,440.00 | ||||||||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7244 Middlebrook 7H11 Agar | ||||||||||||||||||||||||||||||
| Package Weight | 14.00 lb |
GC Agar
GC Agar is used with lysed blood and other supplements for the isolation and cultivation of Neisseria gonorrhoeae and other fastidious organisms. GC Agar is not intended for use in the diagnosis of disease or other conditions in humans.
A nutritious agar base described by Thayer and Martin for the isolation of Neisseria gonorrhoeae. The rich peptone mixture is enhanced by the use of corn starch to absorb toxic metabolites and a buffering system is used to maintain neutral pH. The medium is made selective by the use of various antibiotic cocktails. Thayer and Martin originally recommended the use of vancomycin, colistin and nystatin and the addition of trimethoprim which is useful in preventing the swarming of proteus. More recently the emergence of vancomycin sensitive gonococci has made the selective agents (lincomycin, colistin, amphotericin, trimethoprim (X070 LCAT) the combination of choice. Enrichment of the base is usually by the addition of lysed blood. Alternatively, chocolated blood or hemoglobin powder and Thayer and Martin’s mixture of vitamins, amino acids and coenzymes can be used. GC Growth Supplement (X271) can be added to this medium to aid in the isolation of Neisseria spp.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0045B | |
| 10 kg | NCM0045C | |
| 500 g | NCM0045A |
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Neisseria spp. | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 36.00 | ||||||||||||||
| Liters per 500 g | 14.00 | ||||||||||||||
| Plates per 500 g | 760.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7104 GC Agar; and LabM Product: LAB067 GC Agar Base | ||||||||||||||
| Package Weight | 14.00 lb |
Yeast Extract
Yeast Extract is an autolysate of yeast cells used in preparing microbiological culture media in a laboratory setting. Yeast Extract is not intended for use in the diagnosis of disease or other conditions in humans. Yeast Extract is the water-soluble portion of autolyzed yeast. The autolysis is carefully controlled to preserve naturally occurring B-complex vitamins. Yeast Extract is prepared and standardized for bacteriological use and cell cultures and is an excellent stimulator of bacterial growth. Yeast Extract is generally employed in the concentration of 0.3% – 0.5%. Yeast Extract is typically prepared by growing baker’s yeast, Saccharomyces spp., in a carbohydrate-rich plant medium. The yeast is harvested, washed, and resuspended in water, where it undergoes autolysis, i.e., self-digestion using the yeast’s enzymes. Yeast Extract is the total soluble portion of this autolytic action. The autolytic activity is stopped by a heating step. The resulting Yeast Extract is filtered clear and dried into a powder by spray drying. Yeast Extract has been successful in culture media for bacterial studies in milk and other dairy products. Several media containing Yeast Extract have been recommended for cell culture applications.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0218B | |
| 10 kg | NCM0218C | |
| 25 kg | NCM0218D | |
| 50 kg | NCM0218E | |
| 500 g | NCM0218A |
| Brand | Neogen® Culture Media |
| Analyte | Non-selective |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as Acumedia Product: 7184 Yeast Extract; and LabM Product: MC001 Yeast Extract Powder |
| Package Weight | 14.00 lb |
Tryptic Soy Broth (Soybean-Casein Digest Broth)
Tryptic Soy Broth (Soybean-Casein Digest Broth) is used for the cultivation of a wide variety of microorganisms. Tryptic Soy Broth conforms to Harmonized USP/EP/JP requirements and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptic Soy Broth, a general purpose medium, is commonly referred to as Casein Soya Bean or Soybean-Casein Digest broth, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry. TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines. TSB conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). The rich nutritional base of TSB, supplemented with SPS and CO2, is an excellent broth for blood cultures. With the addition of 6.5% NaCl, TSB can be used for the selective growth of group D streptococci. Enzymatic Digest of Casein and Enzymatic Digest of Soybean Meal are nitrogen sources in TSB. Dextrose is the carbon energy source that facilitates organism growth. Sodium Chloride maintains osmotic balance; Dipotassium Phosphate is a buffering agent.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0004B | |
| 10 kg | NCM0004C | |
| 25 kg | NCM0004D | |
| 50 kg | NCM0004E | |
| 500 g | NCM0004A |
| Brand | Neogen® Culture Media | ||||||||||||
| Analyte | Non-selective | ||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||
| Mass | 5 kg | ||||||||||||
| Formula |
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| Grams per Liter | 30.00 | ||||||||||||
| Liters per 500 g | 17.00 | ||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7164 Tryptic Soy Broth; and LabM Product: HP002 Casein Soya Bean Digest Broth (USP/EP/JP) | ||||||||||||
| Package Weight | 14.00 lb |
Columbia Blood Agar Base
Columbia Blood Agar Base is used with blood for the isolation and cultivation of a wide variety of fastidious microorganisms. Columbia Blood Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. A modification of the original Columbia Agar base formulation, Columbia Blood Agar Base provides a medium that is suitable for use with both defibrinated horse or sheep blood. The peptone mixture provides a source of nitrogen, essential vitamins and amino acids. The starch provides a carbon source and sodium chloride maintains osmotic balance. Originally described as a general purpose nutritious agar base by Ellner et al. Columbia Agar is more frequently used when enriched by the addition of sterile blood. The medium is suitable for supporting the growth and determining hemolytic reactions of a variety of microorganisms.
| Item | SKU No. | |
|---|---|---|
| 10 kg | NCM0031C | |
| 25 kg | NCM0031D | |
| 500 g | NCM0031A |
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Non-selective | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 10 kg | ||||||||||
| Formula |
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| Grams per Liter | 43.10 | ||||||||||
| Liters per 500 g | 12.00 | ||||||||||
| Plates per 500 g | 630.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7125 Columbia Blood Agar Base; and LabM Product: LAB215 Columbia II Agar Base | ||||||||||
| Package Weight | 26.60 lb |
Letheen Broth Base without Tween
Letheen Broth Base (no tween) is used with Tween 80 (Polysorbate 80) for the testing of samples containing quaternary ammonium compounds for antimicrobial activity in a laboratory setting. Letheen Broth Base (no tween) is not intended for use in the diagnosis of disease or other conditions in humans.
In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds while testing germicidal activity. Letheen Agar is a modification of TGE Agar with the addition of Lecithin and Polysorbate 80.
Letheen Broth Base was developed as a subculture medium for the neutralization of quaternary ammonium compounds in disinfectant testing. Quisno, Gibby, and Foter discovered that adding Lecithin and Polysorbate 80 to F.D.A. Broth resulted in a medium that neutralized high concentrations of quaternary ammonium salts. The resulting medium, termed “Letheen” (a combination of Lecithin and Tween) was easy to prepare and clear in appearance, aiding to visual inspection for growth. Letheen Broth Base is recommended by the Official Methods of Analysis of the Association of Official Analytical Chemists (AOAC) for use with disinfectants containing cationic surface active materials.
Letheen Broth Base is specified for use by the American Society for Testing Materials (ASTM) in Standard Test Method for Preservatives in Water-Containing Cosmetics. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0145B | |
| 500 g | NCM0145A |
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Non-selective | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 5 kg | ||||||||||
| Formula |
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| Grams per Liter | 20.70 | ||||||||||
| Liters per 500 g | 24.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7105 Letheen Broth Base (no Tween) | ||||||||||
| Package Weight | 14.00 lb |
D/E Neutralizing Broth with Tween
D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth is not intended for use in the diagnosis of disease or other conditions in humans. D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false-negative (no-growth) test. D/E Neutralizing Broth w/Tween effectively neutralizes the inhibitor action of disinfectant carryover, allowing differentiation between bacteriostasis and the true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralizing Broth w/Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0047B | |
| 10 kg | NCM0047C | |
| 500 g | NCM0047A |
| Brand | Neogen® Culture Media | ||||||||||||||||||||
| Analyte | Non-selective | ||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||
| Formula |
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| Grams per Liter | 39.00 | ||||||||||||||||||||
| Liters per 500 g | 13.00 | ||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7705 D/E Neutralizing Broth with Tween; and LabM Product: LAB187 D/E Neutralising Broth (Dey & Engley) | ||||||||||||||||||||
| Package Weight | 14.00 lb |
Letheen Broth with Tween
Letheen Broth is primarily used for assessing the bactericidal activity of quaternary ammonium compounds, and is not intended for use in the diagnosis of disease or other conditions in humans. Letheen Broth is used to determine the phenol co-efficient of cationic surfactants as recommended by the Official Methods of Analysis of the Association of Official Analytical Chemists (AOAC). It is also used in hygiene swabbing protocols where it is necessary to neutralize quaternary ammonium compounds. A modification of FDA Broth, Letheen Broth contains lecithin to neutralize quaternary ammonium compounds and Polysorbate 80 to neutralize phenols, hexachlorophene, formalin and (with lecithin) ethanol. Letheen Broth is easily prepared and has a clear appearance aiding in visual inspection for growth. The American Society for Testing Materials (ASTM) specifies the use of Letheen Broth in the Standard Test Method for Preservatives in Water Containing Cosmetics.
| Item | SKU No. | |
|---|---|---|
| 10 kg | NCM0116C | |
| 500 g | NCM0116A |
| Brand | Neogen® Culture Media | ||||||||||||
| Analyte | Non-selective | ||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||
| Mass | 10 kg | ||||||||||||
| Formula |
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| Grams per Liter | 25.70 | ||||||||||||
| Liters per 500 g | 19.00 | ||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as LabM Product: LAB184 Letheen Broth | ||||||||||||
| Package Weight | 26.60 lb |
Letheen Broth with Tween, Modified
Letheen Broth with Tween, Modified is used for the recovery of microorganisms from cosmetics in a laboratory setting and is not intended for use in the diagnosis of disease or other conditions in humans.
In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical as disinfectant residues can result in a false negative (no-growth) test result.
Letheen Broth with Tween, Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base. This medium is recommended by the FDA for use in the microbiological testing of cosmetics. Letheen Broth with Tween, Modified has Polysorbate 80 (Tween) incorporated into the medium.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0104B | |
| 10 kg | NCM0104C | |
| 500 g | NCM0104A |
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Non-selective | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 42.80 | ||||||||||||||
| Liters per 500 g | 12.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7712 Letheen Broth with Tween, Modified | ||||||||||||||
| Package Weight | 14.00 lb |
Tryptic Soy Agar with Lecithin and Tween 80
Tryptic Soy Agar with Lecithin and Tween 80 is used for the isolation of microorganisms from surfaces sanitized with quaternary ammonium compounds and is not intended for use in the diagnosis of disease or other conditions in humans.
In 1955, Leavitt et al. discovered Tryptic Soy Agar supported excellent growth of aerobic and anaerobic microorganisms. Tryptic Soy Agar is a nutritious base and a variety of supplements are added to enhance the medium, including Lecithin and Tween 80. The Lecithin and Tween 80 inactivate some preservatives that may inhibit bacterial growth, reducing “preservative carryover”. Tryptic Soy Agar with Lecithin and Tween 80 is recommended for determining the sanitation efficiency of containers, equipment, and work area (environmental monitoring).
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0011B | |
| 10 kg | NCM0011C | |
| 50 kg | NCM0011E | |
| 500 g | NCM0011A |
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Non-selective | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 51.20 | ||||||||||||||
| Liters per 500 g | 11.00 | ||||||||||||||
| Plates per 500 g | 600.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7163 Tryptic Soy Agar with Lecithin & Tween 80; and LabM Product: LAB189 Microbial Content Test Agar (MCTA) | ||||||||||||||
| Package Weight | 14.00 lb |
LB Agar, Miller
LB Agar (Miller), a nutritionally rich medium designed for growth of pure cultures of recombinant strains, is based on the formulation described by Miller. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to a high copy number and do not require selective amplification. Some vectors do not replicate so freely and need to be selectively amplified. Chloramphenicol may be added to inhibit host synthesis and prevent replication of the bacterial chromosome.
LB Agar, Miller contains 10 g/L of sodium chloride, different from the levels in Lennox formulations. This allows the researcher to select the optimal salt concentration for a specific strain. The medium may be aseptically supplemented with glucose or antibiotics.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0142B | |
| 10 kg | NCM0142C | |
| 500 g | NCM0142A |
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Non-selective | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 5 kg | ||||||||||
| Formula |
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| Grams per Liter | 40.00 | ||||||||||
| Liters per 500 g | 13.00 | ||||||||||
| Plates per 500 g | 680.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7213 LB Agar (Miller); and LabM Product: LAB168 LB Agar (Miller) | ||||||||||
| Package Weight | 14.00 lb |
SIM Medium
SIM Medium is used for the differentiation of microorganisms on the basis of hydrogen sulfide production, indole production, and motility in a laboratory setting. SIM Medium is not intended for use in the diagnosis of disease or other conditions in humans.
Semisolid media have been used extensively in the determination of bacterial motility. The production of hydrogen sulfide, indole formation, and motility are useful diagnostic tests in the identification of Enterobacteriaceae, especially Salmonella spp. and Shigella spp.
In 1940, Sulkin and Willett demonstrated motility, hydrogen sulfide production, and carbohydrate fermentation by members of Salmonella and Shigella groups. They called attention to the “brush-like growth” or motility of the typhoid organisms. Greene et al. used SIM Medium to detect motility in a large series of cultures of typhoid organisms.
| Brand | Neogen® Culture Media | ||||||||||||
| Analyte | Non-selective | ||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||
| Mass | 500 g | ||||||||||||
| Formula |
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| Grams per Liter | 30.00 | ||||||||||||
| Liters per 500 g | 17.00 | ||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7221 SIM Medium | ||||||||||||
| Package Weight | 15.30 lb |
Urea Agar Base (Christensen)
Urea Agar Base is used with agar for the differentiation of microorganisms on the basis of urease production in a laboratory setting. Urea Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
Christensen devised a urea agar medium containing peptone and dextrose that had a reduced buffer content. The medium supported the vigorous growth of many Gram-negative, enteric bacilli and readily permitted observation of urease production. Urea Agar Base may be used as a screening medium (along with Triple Sugar Iron Agar) for the selection of Salmonella and Shigella cultures for serologic classification. Urea Agar Base is also used to detect production of urease by yeast. Urease production is an important differential test in microbiology and outlined in standard methods.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0180B | |
| 500 g | NCM0180A |
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Non-selective | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 21.00 | ||||||||||||||
| Liters per 500 g | 24.00 | ||||||||||||||
| Plates per 500 g | 1,300.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7226 Urea Agar Base; and LabM Product: LAB130 Urea Agar Base | ||||||||||||||
| Package Weight | 15.30 lb |
Letheen Broth Base Modified without Tween
Letheen Broth Base Modified without Tween is used with Polysorbate 80 for the recovery of microorganisms from cosmetics in a laboratory setting. Letheen Broth Base Modified without Tween is not intended for use in the diagnosis of disease or other conditions in humans.
In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Polysorbate 80 to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.
Letheen Broth Base Modified without Tween is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Broth Base. Letheen Broth Base Modified is recommended by the FDA for use in the microbiological testing of cosmetics.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0129B | |
| 10 kg | NCM0129C | |
| 500 g | NCM0129A |
| Brand | Neogen® Culture Media | ||||||||||||
| Analyte | Non-selective | ||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||
| Mass | 5 kg | ||||||||||||
| Formula |
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| Grams per Liter | 37.80 | ||||||||||||
| Liters per 500 g | 13.00 | ||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7496 Letheen Broth Base, Modified | ||||||||||||
| Package Weight | 14.00 lb |
Sodium Chloride (Bacteriological)
Sodium Chloride is a salt often used when preparing microbiological culture media in a laboratory setting. Sodium Chloride is not intended for use in the diagnosis of disease or other conditions in humans.
When added to culture media Sodium Chloride allows for adjustments in osmotic pressure.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0245B | |
| 10 kg | NCM0245C | |
| 25 kg | NCM0245D | |
| 500 g | NCM0245A |
| Brand | Neogen® Culture Media |
| Analyte | Non-selective |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC017 Sodium Chloride (Bacteriological) |
| Package Weight | 15.30 lb |
Simmons Citrate Agar
Simmons Citrate Agar is used for the differentiation of microorganisms on the basis of citrate utilization in a laboratory setting. Simmons Citrate Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Koser first developed a liquid medium for differentiating coliforms from fecal coliforms. Fecal coliforms were unable to use citrate as the sole source of carbon and inorganic ammonium salt as a sole source of nitrogen. Non-fecal coliforms, such as Enterobacter aerogenes could use citrate in such a medium with resultant alkalinity. Liquid medium had the disadvantage of appearing turbid when a large inoculum was used, although no growth had taken place. This observation led Simmons to devise a solid medium that eliminated the problem with turbidity.
Simmons Citrate Agar is a modification of Koser’s medium, with the addition of bromothymol blue and 1.5% agar. Organisms able to metabolize citrate grow luxuriantly. The medium is alkalinized and changes from green to deep blue in 24 – 48 hours. Escherichia coli either do not grow at all on this medium or grow so sparsely that no change in reaction is apparent. Simmons Citrate Agar is recommended for differentiation of enteric Gram-negative bacilli from laboratory specimens, water samples, and food samples.
| Brand | Neogen® Culture Media | ||||||||||||||||
| Analyte | Non-selective | ||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||
| Mass | 500 g | ||||||||||||||||
| Formula |
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| Grams per Liter | 24.20 | ||||||||||||||||
| Liters per 500 g | 21.00 | ||||||||||||||||
| Plates per 500 g | 1,130.00 | ||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7156 Simmons Citrate Agar; and LabM Product: LAB069 Simmons Citrate Agar | ||||||||||||||||
| Package Weight | 1.35 lb |
Nutrient Agar 1.5%
Nutrient Agar 1.5% is used for the cultivation of a wide variety of microorganisms in a laboratory setting. Nutrient Agar 1.5% is not intended for use in the diagnosis of disease or other conditions in humans.
In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Agar is specified in many standard method procedures. Nutrient Agar 1.5% is a modification of Nutrient Agar. Nutrient Agar 1.5% is a general purpose medium, with a slightly alkaline pH. This medium contains 0.8% sodium chloride and can be used as a base for enrichment with blood, ascitic fluid, or other supplements for cultivating fastidious microorganisms.
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Non-selective | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 10 kg | ||||||||||
| Formula |
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| Grams per Liter | 31.00 | ||||||||||
| Liters per 500 g | 16.00 | ||||||||||
| Plates per 500 g | 880.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7286 Nutrient Agar 1.5% | ||||||||||
| Package Weight | 26.60 lb |
D/E Neutralizing Broth without Tween
D/E Neutralizing Broth is used to neutralize and determine the bactericidal activity of antiseptics and disinfectants. D/E Neutralizing Broth without Tween is not intended for use in the diagnosis of disease or other conditions in humans. D/E Neutralizing Broth was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycollate media, and Neutralizing Buffer. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false-negative (no-growth) test. D/E Neutralizing Broth without Tween effectively neutralizes the inhibitor action of disinfectant carryover, allowing differentiation between bacteriostasis and the true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralizing Broth without Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0121B | |
| 10 kg | NCM0121C | |
| 500 g | NCM0121A |
| Brand | Neogen® Culture Media | ||||||||||||||||||
| Analyte | Non-selective | ||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||
| Formula |
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| Grams per Liter | 34.00 | ||||||||||||||||||
| Liters per 500 g | 13.00 | ||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7562 DE Neutralizing Broth (no tween) | ||||||||||||||||||
| Package Weight | 14.00 lb |
Nutrient Broth
Nutrient Broth is used for the cultivation of a wide variety of microorganisms. Nutrient Broth is not intended for use in the diagnosis of disease or other conditions in humans. In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Broth is the same formulation as Nutrient Agar, only Agar has been omitted. Nutrient Broth is used as a pre-enrichment medium when testing certain foods and dairy products for Salmonella spp. In dried or processed foods, salmonellae may be sub-lethally injured and in low numbers. The presence of other bacteria and food sample components may hinder growth and recovery of Salmonella spp. Pre-enrichment in a nonselective medium such as Nutrient Broth allows for cell damage repair, dilutes toxic or inhibitory substances, and provides a nutritional advantage to Salmonella over other bacteria. Nutrient Broth is included in many standard methods procedures for testing food, dairy products, and other materials.
| Brand | Neogen® Culture Media | ||||||
| Analyte | Non-selective | ||||||
| Platform | Dehydrated Culture Media | ||||||
| Mass | 5 kg | ||||||
| Formula |
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| Grams per Liter | 8.00 | ||||||
| Liters per 500 g | 38.00 | ||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7146 Nutrient Broth; and LabM Product: LAB068 Nutrient Broth ‘E’ | ||||||
| Package Weight | 14.00 lb |
Nutrient Agar (BAM)
Nutrient Agar (BAM) is used for the cultivation of a wide variety of microorganisms in a laboratory setting. Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans.
In the early 1900’s, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Agar continues to be a widely used general purpose medium for growing non-fastidious microorganisms. If required, enrichments can be added to this medium. Nutrient Agar, modified by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG), is used for fluorogenic detection of Escherichia coli.
Nutrient Agar meets APHA and Association of Official Analytical Chemists (AOAC) standard methods. Nutrient Agar is specified in many standard methods procedures for the examination of food, dairy products, water, and other materials.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0269B | |
| 500 g | NCM0269A |
| Brand | Neogen® Culture Media | ||||||||
| Analyte | Non-selective | ||||||||
| Platform | Dehydrated Culture Media | ||||||||
| Mass | 5 kg | ||||||||
| Formula |
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| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7145 Nutrient Agar (BAM) | ||||||||
| Package Weight | 15.30 lb |
Mueller Hinton Agar II
Mueller Hinton Agar II is used in antimicrobial susceptibility testing by the disk diffusion method. This formula conforms to Clinical and Laboratory Standard Institute (CLSI), formerly National Committee for Clinical Laboratory Standards (NCCLS).
Mueller Hinton Agar II is based on the formula recommended by Mueller and Hinton for the primary isolation of Neisseria species. Mueller and Hinton selected pea meal extract agar as a simple transparent medium containing heat stable ingredients. During their modification, starch replaced the growth-promoting properties of pea extract, acting as a “protective colloid” against toxic substances.
Bauer, Kirby, Sherris and Tuck recommended Mueller Hinton Agar for performing antibiotic susceptibility tests using a single disk of high concentration. This un-supplemented medium has been selected by the Clinical and Laboratory Standard Institute (CLSI) for several reasons. This medium is low in sulfonamide, trimethoprim and tetracycline inhibitors, and provides satisfactory growth of most non-fastidious pathogens along with demonstrating batch-to-batch reproducibility.
Mueller Hinton Agar is often abbreviated as M-H Agar and complies with requirements of the World Health Organization. Mueller Hinton Agar is specified in FDA Bacteriological Analytical Manual for food testing, and procedures commonly performed on aerobic and facultatively anaerobic bacteria. A variety of supplements can be added to Mueller Hinton Agar, including 5% defibrinated sheep or horse blood, 1% growth supplement and 2% sodium chloride.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0023B | |
| 10 kg | NCM0023C | |
| 50 kg | NCM0023E | |
| 500 g | NCM0023A |
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Non-selective | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 5 kg | ||||||||||
| Formula |
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| Grams per Liter | 38.00 | ||||||||||
| Liters per 500 g | 13.00 | ||||||||||
| Plates per 500 g | 720.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7101 Mueller Hinton Agar | ||||||||||
| Package Weight | 14.00 lb |
Plate Count Agar (Standard Methods)
Plate Count Agar (Standard Methods Agar) is used for the enumeration of bacteria in water, wastewater, food, and dairy products in a laboratory setting. Plate Count Agar is not intended for use in the diagnosis of disease or other conditions in humans. This formula conforms to ISO 4833-1&2:2013, ISO 17410:2001, American Public Health Association (APHA), Association of Official Analytical Chemists (AOAC) and FDA Bacteriological Analytical Manual. Plate Count Agar (Standard Methods Agar) is free from selective supplements and relatively rich in nutrients, making it ideal for the enumeration of viable organisms, either following a pour plate method, or a surface plate method which can be coupled with a spiral plater. Plate Count Agar (Standard Methods Agar) is also referred to as Tryptone Glucose Yeast Agar. This formula is specified in standard method procedures.
| Item | SKU No. | |
|---|---|---|
| 10 kg | NCM0010C | |
| 500 g | NCM0010A |
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Non-selective | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 10 kg | ||||||||||
| Formula |
* 9 – 18 g according to gel strength | ||||||||||
| Grams per Liter | 23.50 | ||||||||||
| Liters per 500 g | 21.00 | ||||||||||
| Plates per 500 g | 1,160.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7157 Standard Methods Agar; and LabM Products: LAB010 Plate Count Agar A.P.H.A and LAB149 Plate Count Agar | ||||||||||
| Package Weight | 26.60 lb |
Tryptic Soy Agar (Soybean-Casein Digest Agar)
Tryptic Soy Agar is used for the preparation and maintenance of test strains used in growth promotion tests, suitability of the counting methods and as negative controls as described in the Harmonized USP/EP/JP. It is also a support plating medium for various protocols described in the microbial enumeration test section of the Harmonized USP/EP/JP. Tryptic Soy Agar is not intended for use in the diagnosis of disease or other conditions in humans. Enzymatic digests of casein and soybean act as a source of nitrogen and amino acid and sodium chloride maintains the osmotic balance.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0002B | |
| 10 kg | NCM0002C | |
| 25 kg | NCM0002D | |
| 50 kg | NCM0002E | |
| 500 g | NCM0002A |
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Non-selective | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 5 kg | ||||||||||
| Formula |
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| Grams per Liter | 40.00 | ||||||||||
| Liters per 500 g | 13.00 | ||||||||||
| Plates per 500 g | 680.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7100 Tryptic Soy Agar; and LabM Product: HP016 Casein Soya Bean Digest Agar (USP/EP/JP) | ||||||||||
| Package Weight | 14.00 lb |
Buffered Sodium Chloride - Peptone Broth pH 7.0
Buffered Sodium Chloride-Peptone Broth pH 7.0 is the diluent specified by the Harmonized Pharmacopoeia for the microbiological examination of non-sterile products andconforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Buffered Sodium Chloride-Peptone Broth pH 7.0 is not intended for use in the diagnosis of disease or other conditions in humans.
Buffered Sodium Chloride-Peptone Broth pH 7.0 is recommended by the Harmonized Pharmacopoeia (USP/EP/JP) for the microbiological examination of non-sterile products. The low level of peptone lessens the physiological shock experienced by microorganism when suspended in a diluent. The dual phosphate components create a buffered environment and sodium chloride maintains the osmotic balance.
| Item | SKU No. | |
|---|---|---|
| 10 kg | NCM0156C | |
| 50 kg | NCM0156E | |
| 500 g | NCM0156A |
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Non-selective | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Mass | 10 kg | ||||||||||
| Formula |
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| Grams per Liter | 14.70 | ||||||||||
| Liters per 500 g | 31.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7732 Buffered Sodium Chloride – Peptone Solution, pH 7.0; and LabM Product: HP017 Buffered Sodium Chloride-Peptone Solution pH 7.0 (USP/EP/JP) | ||||||||||
| Package Weight | 26.60 lb |
LB Broth (Miller)
LB Broth (Miller) is used in molecular genetic studies in a laboratory setting. LB Broth, Miller is not intended for use in the diagnosis of disease or other conditions in humans. LB Broth (Miller) is based on the formula described by Miller. This medium is used for the growth and maintenance of Escherichia coli strains used in molecular microbiology procedures. LB Broth, Miller is a nutritionally rich medium designed for growth of pure cultures of recombinant strains. E. coli is grown to late log phase in LB Medium. Some plasmid vectors replicate to high copy numbers without selective amplification. Some vectors do not replicate so freely and need to be selectively amplified. Chloramphenicol can be added to inhibit host synthesis and prevent replication of the bacterial chromosome. This broth contains a high level of sodium chloride to aid the maintenance of plasmids. If working with temperate bacteriophages, such as lambda, the addition of magnesium sulphate at 2g/l is recommended to promote phage absorption. If required the medium may be aseptically supplemented with glucose after sterilization.
| Brand | Neogen® Culture Media | ||||||||
| Analyte | Non-selective | ||||||||
| Platform | Dehydrated Culture Media | ||||||||
| Mass | 500 g | ||||||||
| Formula |
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| Grams per Liter | 25.00 | ||||||||
| Liters per 500 g | 20.00 | ||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7279 LB Broth, Miller; and LabM Product: LAB169 L.B. Broth | ||||||||
| Package Weight | 1.35 lb |
MacConkey Agar without Crystal Violet
MacConkey Agar without Crystal Violet is used for the isolation and differentiation of Gram-negative enteric bacilli and is not intended for use in the diagnosis of disease or other conditions in humans.
A selective medium for the isolation of bile tolerant organisms from sewage and foodstuffs. Bile-tolerant Gram positive organisms as well as Gram negative organisms will grow on this medium. This formula is recommended by W.H.O. and other bodies for the examination of water and milk. Some strains of Proteus spp. will spread on this medium making interpretation difficult, for this reason MacConkey Agar (without salt) may be preferred as it is less prone to this phenomenon.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0160B | |
| 500 g | NCM0160A |
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Non-selective | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 52.00 | ||||||||||||||
| Liters per 500 g | 10.00 | ||||||||||||||
| Plates per 500 g | 520.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7236 MacConkey Agar without Crystal Violet; and LabM Product: LAB030 MacConkey Agar without Crystal Violet | ||||||||||||||
| Package Weight | 15.30 lb |
Tryptose No. 2
Tryptose No. 2 is a special blend of peptones developed for use in the production of Foot and Mouth vaccine, and is not intended for use in the diagnosis of disease or other conditions in humans. A special blend of peptones originally developed for use in the production of Foot and Mouth vaccine. Other peptone blends are also available within the catalogue, some of which have been evaluated to demonstrate low cell toxicity and the ability to enhance the growth of cell lines (eg NCM0264).
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0260B | |
| 10 kg | NCM0260C | |
| 500 g | NCM0260A |
| Brand | Neogen® Culture Media |
| Analyte | Non-selective |
| Platform | Dehydrated Culture Media |
| Mass | 5 kg |
| Previous Item Number | Product was previously known as LabM Product: MC033 Tryptose No. 2 |
| Package Weight | 15.30 lb |
Letheen Agar Base Modified without Tween
Letheen Agar Base Modified (without Tween) is used with Tween 80 (Polysorbate 80) for the isolation of microorganisms from cosmetics in a laboratory setting. Letheen Agar Base Modified is not intended for use in the diagnosis of disease or other conditions in humans.
In 1948, Weber and Black described the value of a highly nutritional solid medium containing neutralizing agents for quaternary ammonium compounds in sanitizers. The addition of Lecithin and Tween 80 (Polysorbate 80) to Tryptone Glucose Extract (TGE) Agar resulted in a medium that effectively neutralizes quaternary ammonium compounds in testing of germicidal activity. Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (no-growth) test.
Letheen Agar Base Modified is based on the formula described in FDA Bacteriological Analytical Manual, and a modification of Letheen Agar Base. Letheen Agar Base Modified is recommended by the FDA for use in the microbiological testing of cosmetics.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0260B | |
| 10 kg | NCM0260C | |
| 500 g | NCM0260A |
| Brand | Neogen® Culture Media | ||||||||||||||
| Analyte | Non-selective | ||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||
| Mass | 5 kg | ||||||||||||||
| Formula |
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| Grams per Liter | 52.10 | ||||||||||||||
| Liters per 500 g | 10.00 | ||||||||||||||
| Plates per 500 g | 520.00 | ||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7496 Letheen Broth Base, Modified | ||||||||||||||
| Package Weight | 14.00 lb |
Maximum Recovery Diluent (Tryptone Salt Broth)
Maximum Recovery Diluent (Tryptone Salt Broth) is used as an isotonic diluent for maximum recovery of microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans. An osmotically controlled solution which is an alternative to, and a replacement for, 1/4 strength Ringer’s Tablets (NCM0191K). The presence of a low level of peptone lessens the physiological shock normally experienced by bacterial cells when they are introduced to a diluent such as Ringer’s Solution. The level of peptone is such that multiplication of the organisms is not possible in the time in which the sample will be present in the diluent (1-2 hours). This formula is recommended by ISO 6887-1:2017
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0085B | |
| 10 kg | NCM0085C | |
| 500 g | NCM0085A |
| Brand | Neogen® Culture Media | ||||||
| Analyte | Non-selective | ||||||
| Platform | Dehydrated Culture Media | ||||||
| Mass | 5 kg | ||||||
| Formula |
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| Grams per Liter | 9.50 | ||||||
| Liters per 500 g | 53.00 | ||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7658 Maximum Recovery Diluent (Peptone Saline Diluent); and LabM Product: LAB103 Maximal Recovery Diluent | ||||||
| Package Weight | 15.30 lb |
Tryptic Soy Blood Agar Base No.2
Tryptic Soy Blood Agar Base No. 2 is used with blood for the isolation and cultivation of a wide variety of microorganisms in a laboratory setting. Tryptic Soy Blood Agar Base No. 2 is not intended for use in the diagnosis of disease or other conditions in humans.
Blood Agar Bases are typically supplemented with 5 – 10% sheep, rabbit, or horse blood, and used for isolating, cultivating, and determining hemolytic reactions of fastidious pathogenic microorganisms. Without enrichment, this blood agar base can be used as a general purpose medium.
Tryptic Soy Agar is based on the Soybean-Casein Digest formula specified by US Pharmacopeia. Tryptic Soy Agar is a nutritious base, and a variety of supplements, including blood, are often added to enhance this medium. Tryptic Soy Blood Agar Base No. 2 was formulated to achieve good growth and improve hemolytic reactions of important pathogenic bacteria. Blood Agar Base media are specified in standard methods for food testing.
| Item | SKU No. | |
|---|---|---|
| 500 g | NCM0098A | |
| 10 kg | NCM0098C |
| Brand | Neogen® Culture Media | ||||||||||
| Analyte | Non-selective | ||||||||||
| Platform | Dehydrated Culture Media | ||||||||||
| Formula |
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| Grams per Liter | 40.00 | ||||||||||
| Liters per 500 g | 13.00 | ||||||||||
| Plates per 500 g | 680.00 | ||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7654 Tryptic Soy Blood Agar Base No. 2 | ||||||||||
| Package Weight | 1.35 lb |
LB Broth, Lennox
LB Broth, Lennox is used in molecular genetic studies in a laboratory setting. LB Broth, Lennox is not intended for use in the diagnosis of disease or other conditions in humans.
LB Broth formula is based on L Broth described by Lennox for growth and maintenance of E. coli strains used in molecular microbiology procedures. LB Broth is nutritionally rich, formulated for the isolation of pure recombinant strains. E. coli is grown to late log phase in LB Broth. Some plasmid vectors may replicate to high copy numbers without selective amplification. Some vectors may require selective amplification to reach high copy numbers. Chloramphenicol can be added to inhibit host synthesis and, as a result, prevent replication of the bacterial chromosome.
LB Broth contains ten times the sodium chloride level of Luria Broth, Miller and one half of that found in LB Broth, Miller. This permits the researcher to select the optimal salt concentration for a specific strain. If desired, the medium may be aseptically supplemented with glucose to prepare the complete medium described by Lennox.
HC Agar Base is used with Tween 80 (Polysorbate 80) for the enumeration of molds in cosmetics in a laboratory setting. HC Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.
Methods for isolating molds from cosmetic products require incubation for 5 – 7 days using traditional agar media. In 1986, Mead and O’Neill described a new medium, HC Agar, for enumerating molds in cosmetic products. The formulation of HC Agar decreased incubation time to 3 days at 27.5 ± 0.5°C for molds. HC Agar Base, based on the HC Agar formula of Mead and O’Neill, is supplemented with Tween 80 (Polysorbate 80) to prepare HC Agar.
| Brand | Neogen® Culture Media | ||||||||
| Analyte | Non-selective | ||||||||
| Platform | Dehydrated Culture Media | ||||||||
| Mass | 500 g | ||||||||
| Formula |
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| Grams per Liter | 20.00 | ||||||||
| Liters per 500 g | 25.00 | ||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7290 LB Broth, Lennox; and LabM Product: LAB173 LB Broth (Lennox) | ||||||||
| Package Weight | 1.35 lb |
Phosphate Buffer, pH 7.2
Phosphate Buffer, pH 7.2 is used for the preparation of microbiological dilution blanks in a laboratory setting. Phosphate Buffer, pH 7.2 is not intended for use in the diagnosis of disease or other conditions in humans.
The formula for phosphate buffer is specified by the American Public Health Association (APHA) for use in diluting test samples. Phosphate Buffer, pH 7.2 is specified for use in diluting water, dairy products and food for microbiological methods. In the examination of water and dairy products the addition of magnesium chloride is recommended.
This buffer is also referred to as Butterfield’s Buffered Phosphate Diluent and recommended for examination of food. Phosphate Buffer, pH 7.2 stabilizes the pH of water used for dilutions.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0223B | |
| 500 g | NCM0223A |
| Brand | Neogen® Culture Media | ||||||
| Analyte | Non-selective | ||||||
| Platform | Dehydrated Culture Media | ||||||
| Mass | 5 kg | ||||||
| Formula |
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| Grams per Liter | 34.00 | ||||||
| Liters per 500 g | 15.00 | ||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7380 Phosphate Buffer, pH 7.2 | ||||||
| Package Weight | 15.30 lb |
Eugon Broth
Eugon Broth is a highly nutritious, general purpose medium used for the cultivation of a wide variety of Microorganisms and is not intended for use in the diagnosis of disease or other conditions in humans.
Eugon Broth is prepared according to the formula defined by Vera. The formula name, Eugon Broth, was used to describe the luxuriant or eugonic growth of fastidious microorganisms. This medium can be used with or without enrichments or supplements for fastidious microorganisms, including Neisseria meningitidis.
| Brand | Neogen® Culture Media | ||||||||||||||||
| Analyte | Non-selective | ||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||
| Mass | 500 g | ||||||||||||||||
| Formula |
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| Grams per Liter | 30.40 | ||||||||||||||||
| Liters per 500 g | 17.00 | ||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 9263 Eugon Broth; LabM Product: LAB526 Eugon Broth | ||||||||||||||||
| Package Weight | 1.35 lb |
TAT Broth
TAT Broth is used for the detection of microorganisms in cosmetics and topical drugs in a laboratory setting. TAT Broth is not intended for use in the diagnosis of disease or other conditions in humans.
TAT Broth is prepared for use in the examination of cosmetics and topical drugs. TAT Broth, with the addition of Polysorbate 20, is recommended for sterility testing of viscous materials, including salves or ointments. It is especially adapted for sterility testing of cosmetics. Cosmetic products are subject to contamination during manufacturing and use by consumers. Preservatives are used in aqueous products to make them self-sterilizing for vegetative bacteria, yeast, and molds. TAT (Tryptone-Azolectin-Tween) Broth is also referred to as Fluid Casein Digest-Soy Lecithin Polysorbate 20 Medium.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0091B | |
| 10 kg | NCM0091C | |
| 500 g | NCM0091A |
| Brand | Neogen® Culture Media | ||||||
| Analyte | Non-selective | ||||||
| Platform | Dehydrated Culture Media | ||||||
| Mass | 5 kg | ||||||
| Formula |
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| Grams per Liter | 25.00 | ||||||
| Liters per 500 g | 20.00 | ||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7219 TAT Broth | ||||||
| Package Weight | 14.00 lb |
D/E Neutralizing Agar with Tween
D/E Neutralizing Agar with Tween is used for the isolation of microorganisms from sanitized environmental surfaces and is not intended for use in the diagnosis of disease or other conditions in humans.
D/E Neutralizing Agar with Tween was developed by Dey and Engley to neutralize a broad spectrum of disinfectants and preservative antimicrobial chemicals, including quaternary ammonium compounds, phenolics, iodine, chlorine preparations, mercurials, formaldehyde, and glutaraldehyde. D/E Neutralizing media neutralize higher concentrations of residual antimicrobials when compared with other standard neutralizing formulations, such as Letheen media, Thioglycolate media, and Neutralizing Buffer.
Total neutralization of disinfectants is critical. Disinfectant residues can result in a false negative (nogrowth) test. D/E Neutralizing Agar effectively neutralizes the inhibitory action of disinfectant carryover, allowing differentiation between bacteriostasis and true bactericidal action of disinfectant chemicals. This is a critical characteristic to consider when evaluating a disinfectant. D/E Neutralizing Agar with Tween is recommended for use in disinfectant evaluations, environmental sampling (swab and contact plate methods), and testing of water-miscible cosmetics.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0009B | |
| 50 kg | NCM0009E | |
| 500 g | NCM0009A |
| Brand | Neogen® Culture Media | ||||||||||||||||||||||
| Analyte | Non-selective | ||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||
| Formula |
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| Grams per Liter | 54.00 | ||||||||||||||||||||||
| Liters per 500 g | 9.00 | ||||||||||||||||||||||
| Plates per 500 g | 500.00 | ||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7375 D/E Neutralising Agar; and LabM Product: LAB188 D/E Neutralising Agar (Dey & Engley) | ||||||||||||||||||||||
| Package Weight | 14.00 lb |
Columbia Broth
Columbia Broth is used for the cultivation of a wide variety of fastidious microorganisms in a laboratory setting. Columbia Broth is not intended for use in the diagnosis of disease or other conditions in humans.
Columbia Broth is prepared according to the formula described by Morello and Ellner. During their study, Columbia Broth was developed as a medium for blood cultures and found superior to a commonly used broth for growth of Staphylococcus aureus, E. coli, and streptococci.
| Item | SKU No. | |
|---|---|---|
| 10 kg | NCM0162C | |
| 500 g | NCM0162A |
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||||
| Analyte | Non-selective | ||||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||||
| Mass | 10 kg | ||||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 35.00 | ||||||||||||||||||||||||||
| Liters per 500 g | 14.00 | ||||||||||||||||||||||||||
| Plates per 500 g | 780.00 | ||||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7127 Columbia Broth | ||||||||||||||||||||||||||
| Package Weight | 26.60 lb |
B-Pep CCT/ET 02
A blended tryptose derived from enzymatic digest of animal origin meat and casein peptones developed as a nutritional supplement for use in animal cell culture applications. B-Pep CCT/ET 02 is not intended for use in the diagnosis of disease or other conditions in humans.
A blended tryptose derived from enzymatic digest of animal origin meat and casein peptones developed as a nutritional supplement for use in animal cell culture applications. Each batch is evaluated to demonstrate low cell toxicity.
| Brand | Neogen® Culture Media |
| Analyte | Non-selective |
| Platform | Dehydrated Culture Media |
| Mass | 25 kg |
| Previous Item Number | Product was previously known as LabM Product: NT004 |
| Package Weight | 61.70 lb |
Dichloran Glycerol (DG-18) Agar Base
Dichloran Glycerol (DG-18) Agar Base is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. Dichloran Glycerol (DG-18) Agar Base is not intended for use in the diagnosis of disease or other conditions in humans. Dichloran Glycerol (DG-18) Agar Base is used for the enumeration of viable osmophilic yeasts and xerophilic molds in food or animal feed products with a water activity of less than or equal to 0.95. This includes such foods as dry fruits, jams, cakes, dried meat, salted fish, grains, cereals, flours, nuts, spices, condiments and some animal feeds. This medium is not suitable for the examination of dehydrated products with a water activity of greater than 0.95, Dichloran Rose Bengal Chloramphenicol Agar (NCM0082 or NCM0029) should be used. The reduction in water activity in this medium is achieved by the addition of glycerol at approximately 18% and this is very important as many yeast and molds require a low water activity to enhance growth and colony development. The medium also contains the antifungal agent dichloran, which restricts the spreading of mucoraceous fungi and restricts the colony size of other genera making colony counting an easier task. Additional selectivity against bacterial growth is achieved by the incorporation of the heat-stable antibiotic Chloramphenicol. Glucose is incorporated as the fermentable carbohydrate source, with casein enzymatic digest providing the essential vitamins, minerals, amino acids, nitrogen and carbon.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0081B | |
| 10 kg | NCM0081C | |
| 500 g | NCM0081A |
| Brand | Neogen® Culture Media | ||||||||||||||||
| Analyte | Osmophilic Yeasts, Xerophilic Moulds | ||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||
| Mass | 5 kg | ||||||||||||||||
| Formula |
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| Grams per Liter | 31.60 | ||||||||||||||||
| Liters per 500 g | 16.00 | ||||||||||||||||
| Plates per 500 g | 860.00 | ||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7592 Dichloran Glycerol (DG-18) Agar Base; and LabM Product: LAB218 DG18 Agar – Dichloran (18%) Glycerol Agar | ||||||||||||||||
| Package Weight | 14.00 lb |
WL Nutrient Agar
W-L Nutrient Agar is used for the cultivation of yeasts, molds, and bacteria encountered in brewing and industrial fermentations. W-L Nutrient Agar is not intended for use in the diagnosis of disease or other conditions in humans.
W-L Nutrient Medium was developed by Green and Gray while studying various fermentation processes. An exhaustive study examining methods of fermentation control procedures in worts, beers, liquid yeasts and similar fermentation products led to the development of W-L Nutrient Medium. At a pH of 5.5, counts of viable baker’s yeast will grow on W-L Nutrient Medium. W-L Nutrient Medium is also referred to as “Wallerstein Laboratory Medium”.
| Item | SKU No. | |
|---|---|---|
| 5 kg | NCM0118B | |
| 500 g | NCM0118A |
| Brand | Neogen® Culture Media | ||||||||||||||||||||||||
| Analyte | Pitching Yeasts, Yeast & Mold | ||||||||||||||||||||||||
| Platform | Dehydrated Culture Media | ||||||||||||||||||||||||
| Mass | 5 kg | ||||||||||||||||||||||||
| Formula |
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| Grams per Liter | 75.00 | ||||||||||||||||||||||||
| Liters per 500 g | 7.00 | ||||||||||||||||||||||||
| Plates per 500 g | 360.00 | ||||||||||||||||||||||||
| Preparation |
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| Previous Item Number | Product was previously known as Acumedia Product: 7488 WL Nutrient Agar; and LabM Product: LAB079 WL Nutrient Agar | ||||||||||||||||||||||||
| Package Weight | 15.30 lb |
