Analytical Enzymes
Asparaginase (Escherichia coli)
Description
Documents
Safety information
Description
| Content: | 400 Units at 25oC |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 2.5 M lithium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Other Activities |
| EC Number: | 3.5.1.1 |
| CAS Number: | 9015-68-3 |
| Synonyms: | asparaginase; L-asparagine amidohydrolase |
| Source: | Escherichia coli |
| Molecular Weight: | 37,900 |
| Concentration: | Supplied at ~ 350 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Catalyses the reaction: L-Asparagine + H2O = L-aspartate + NH3 |
| Specific Activity: | ~ 15 U/mg (25oC, pH 8.0 on L-asparagine); ~ 30 U/mg (37oC, pH 8.0 on L-asparagine) |
| Unit Definition: | One Unit of asparaginase activity is defined as the amount of enzyme required to produce one µmole of L-aspartate from L-asparagine (7.3 mM) per minute in the presence of NADPH in Tris.HCL buffer (45 mM), pH 8.0. |
| Temperature Optima: | 37oC |
| pH Optima: | 8 |
| Application examples: | Applications established for the measurement of asparagine in the food, fermentation, wine, beverage, medical and pharmacological industries. |
Documents
Safety information
| Symbol : | GHS07, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H302, H315, H317, H319, H334, H360 |
| Precautionary Statements : | P201, P202, P261, P264, P270 |
Bromelain from pineapple stems (Ananas comosus
Description
Documents
Safety Information
Description
| Content: | 20 mL; 1,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Protease |
| EC Number: | 3.4.22.32 |
| CAS Number: | 37189-34-7 |
| Source: | Pineapple stems (Ananas comosus) |
| Molecular Weight: | 24,000 |
| Concentration: | Supplied at ~ 55 U/mL |
| Expression: | Purified from Carica papaya |
| Specificity: | A cysteine endopeptidase with a broad specificity for cleavage of proteins. |
| Specific Activity: | ~ 8 U/mg (40oC, pH 7.0 on casein) |
| Unit Definition: | One Unit of activity is the amount of enzyme that will produce one µmole (181 µg) of tyrosine equivalents from casein per minute at pH 7.0 and 40oC (colour determined using the Folin-Ciocalteu reagent). |
| Temperature Optima: | 60oC |
| pH Optima: | 7 |
| Application examples: | For all research and analytical applications. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Acetic Acid Assay Kit (ACS Analyser Format)
Description
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Safety Information
Description
| Content: | 141.6 mL of prepared reagent (e.g. 456 assays of 0.31 mL) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Acetic Acid |
| Assay Format: | Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | up to 30 μg/mL of acetic acid per assay |
| Limit of Detection: | 10 mg/L (recommended assay format) |
| Reaction Time (min): | ~ 15 min |
| Application examples: | Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by EN, ISO, ICUMSA, IFU and MEBAK |
The Acetic Acid analyser format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products.
- No wasted ACS solution (stable suspension supplied)
- PVP incorporated to prevent tannin inhibition
- Very stable reagent when prepared for auto-analyser applications (> 3 days at 4oC)
- Linear calibration up to 30 μg/mL of acetic acid in final reaction solution
- Validated by the University of Wine, Suze la Rousse, France
- Very competitive price (cost per mL of reagent)
- All reagents stable for > 2 years after preparation
Documents
Safety Information
Symbol :Not ApplicableSignal Word :Not ApplicableHazard Statements :Not ApplicablePrecautionary Statements :Not Applicable
Acetaldehyde Assay Kit
Description
Documents
Safety Information
Description
| Content: | 50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 6 months under recommended storage conditions |
| Analyte: | Acetaldehyde |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.5 to 20 µg of acetaldehyde per assay |
| Limit of Detection: | 0.18 mg/L |
| Reaction Time (min): | ~ 4 min |
| Application examples: | Wine, champagne, beer, liqueurs, brandy, dairy products (e.g. yogurt), bread, fruit juices, soft drinks, cocoa, vegetable and fruit products, coffee, and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by MEBAK |
The Acetaldehyde test kit is a simple, reliable and accurate method for the measurement and analysis of acetaldehyde in beverages and foodstuffs.
Documents
Safety Information
Symbol :GHS07Signal Word :WarningHazard Statements :H315, H319Precautionary Statements :P264, P280, P302+P352, P305+P351+P338, P321, P337+P313
Cytidylate kinase (prokaryote)
Description
Documents
Safety Information
Description
| Content: | 500 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Kinase |
| EC Number: | 2.7.4.25 |
| Synonyms: | (d)CMP kinase; ATP:CMP phosphotransferase; UMP-CMP kinase |
| Source: | Prokaryote |
| Molecular Weight: | 26,910 |
| Concentration: | Supplied at ~ 125 U/mL |
| Expression: | Recombinant from a Prokaryotic source |
| Specificity: | Catalyses the reaction: ATP + (d)CMP = ADP + (d)CDP The prokaryotic cytidine monophosphate kinase specifically phosphorylates CMP (or dCMP), using ATP as the preferred phosphoryl donor. |
| Specific Activity: | ~ 50 U/mg (25oC, pH 7.6 on GMP) |
| Unit Definition: | One Unit of cytidylate kinase is defined as the amount of enzyme required to produce one µmole of CDP from CMP and ATP per minute, in the presence of NADH, in TEA buffer (73 mM), pH 7.6 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 7.6 |
| Application examples: | Applications in the measurement of CMP. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Galactose dehydrogenase - Galactose mutarotase
Description
Documents
Safety Information
Description
| Content: | 1 mL; galactose dehydrogenase (200 U/mL) / galactose mutarotase (4 mg/mL) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | Galactose dehydrogenase: 1.1.1.48 Galactose mutarotase: 5.1.3.3 |
| CAS Number: | Galactose dehydrogenase: 9028-54-0 Galactose mutarotase: 9031-76-9 |
| Synonyms: | Galactose dehydrogenase: D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase Galactose mutarotase: aldose 1-epimerase; aldose 1-epimerase |
| Source: | Escherichia coli |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Interconversion of the α- and β-anomeric forms of D-galactose is catalysed by β-galactose mutarotase (GMR) (1). (1) α-D-Galactose ↔ β-D-Galactose. The β-D-Galactose is oxidised by NAD+ to D-galactonic acid in the presence of β-galactose dehydrogenase (GALDH) at pH 7.5 (2). (2) β-D-Galactose + NAD+ → D-galactonic acid + NADH + H+ |
| Specific Activity: | Galactose dehydrogenase: ~ 200 U/mL at pH 8.6 and 25oC Galactose mutarotase: ~ 4 mg/mL |
| Unit Definition: | One Unit of galactose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ per minute at pH 8.6, at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 8.6 |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Galactose dehydrogenase (soil prokaryote)
Description
Documents
Safety Information
Description
| Content: | 200 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.48 |
| CAS Number: | 9028-54-0 |
| Synonyms: | D-galactose 1-dehydrogenase; D-galactose:NAD+ 1-oxidoreductase |
| Source: | Prokaryote |
| Molecular Weight: | 36,659 |
| Concentration: | Supplied at ~ 100 U/mL |
| Expression: | Recombinant from a Soil prokaryote |
| Specificity: | Catalyses the reaction: D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+ Also acts on L-arabinose but more slowly. |
| Specific Activity: | ~ 280 U/mg (25oC, pH 8.6 on D-galactose) |
| Unit Definition: | One unit of galactose dehydrogenase activity is defined as the amount of enzyme required to convert one µmole of D-galactose to D-galactanate per minute in the presence of NAD+ in Tris-HCl buffer (93 mM), pH 8.6 at 25oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 8.6 |
| Application examples: | Applications for the measurement of D-galactose (and L-arabinose) in carbohydrate research and in the food, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
Symbol :Not ApplicableSignal Word :Not ApplicableHazard Statements :Not ApplicablePrecautionary Statements :Not Applicable
Formate dehydrogenase (Candida boidinii)
Description
Documents
Safety Information
Description
| Content: | 300 Units at 25oC; ~ 600 Units at 37oC |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | EC 1.2.1.2 (transferred to EC 1.17.1.9) |
| CAS Number: | 9028-85-7 |
| Synonyms: | formate dehydrogenase; formate:NAD+ oxidoreductase |
| Source: | Candida boidinii |
| Molecular Weight: | 41,331 |
| Concentration: | Supplied at ~ 75 U/mL |
| Expression: | Recombinant from Candida boidinii |
| Specificity: | Catalyses the reaction: Formate + NAD+ = CO2 + NADH |
| Specific Activity: | ~ 2 U/mg (25oC, pH 7.6 on formic acid) |
| Unit Definition: | One Unit of formate dehydrogenase is defined as the amount of enzyme required to convert one µmole of formic acid to NADH + CO2 per minute in the presence of NAD+ in potassium phosphate buffer (41 mM), pH 7.6 at 25oC. |
| Temperature Optima: | 37oC |
| pH Optima: | 7.6 |
| Application examples: | Applications for the measurement of formate in the food, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
Symbol :Not ApplicableSignal Word :Not ApplicableHazard Statements :Not ApplicablePrecautionary Statements :Not Applicable
Guanylate kinase (prokaryote)
Description
Documents
Safety Information
Description
| Content: | 500 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Kinase |
| EC Number: | 2.7.4.8 |
| CAS Number: | 9026-59-9 |
| Synonyms: | guanylate kinase; ATP:(d)GMP phosphotransferase |
| Source: | Prokaryote |
| Molecular Weight: | 25,756 |
| Concentration: | Supplied at ~ 250 U/mL |
| Expression: | Recombinant from a Prokaryotic source |
| Specificity: | Catalyses the reaction: ATP + GMP = ADP + GDP Catalyses the ATP-dependent phosphorylation of Guanosine monophosphate (GMP) into Guanosine diphosphate (GDP). |
| Specific Activity: | ~ 50 U/mg (25oC, pH 7.6 on GMP) |
| Unit Definition: | One Unit of guanylate kinase is defined as the amount of enzyme required to produce one µmole of GDP from GMP and ATP in the presence of NADH in TEA buffer (73 mM), pH 7.6 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 7.6 |
| Application examples: | Applications in the measurement of GMP. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Glucose oxidase - Catalase Mixture (eukaryote)
Description
Documents
Safety Information
Description
| Content: | 2 vials – 200 tests |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | Supplied as a lyophilised powder |
| Physical Form: | Powder |
| Stability: | Minimum 1 year at < -10oC. Check vial for details. |
| Enzyme Activity: | Other Activities |
| EC Number: | Glucose oxidase: 1.1.3.4, Catalase: 1.11.1.16 |
| CAS Number: | Glucose oxidase: 9001-37-0, Catalase: 9001-05-2 |
| Synonyms: | Glucose oxidase: glucose oxidase; beta-D-glucose:oxygen 1-oxidoreductase, Catalase: hydrogen-peroxide:hydrogen-peroxide oxidoreductase |
| Source: | Eukaryote |
| Concentration: | Glucose oxidase (12,000 U) plus Catalase (300,000 U) per vial |
| Expression: | Purified from a Eukaryotic source |
| Specificity: | Catalyses the reactions: (1) D-Glucose + O2 + H20 = D-Gluconate + H2O2 (2) 2H2O2 = 2H2O + O2 |
| Specific Activity: | Glucose oxidase (12,000 U) plus Catalase (300,000 U) per vial |
| Temperature Optima: | 30oC |
| pH Optima: | 7 |
| Application examples: | For use in removal of excess glucose in conjunction with the Sucrose/Glucose |
Documents
Safety Information
Symbol :GHS08Signal Word :DangerHazard Statements :H334Precautionary Statements :P261, P284, P304+P340, P342+P311, P501
Glucose-6-phosphate dehydrogenase (Leuconostoc mesenteroides)
Description
Documents
Safety Information
Description
| Content: | 5,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.49 |
| CAS Number: | 9001-40-5 |
| Synonyms: | glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase |
| Source: | Leuconostoc mesenteroides |
| Molecular Weight: | 54,441 |
| Concentration: | Supplied at ~ 2,000 U/mL |
| Expression: | Purified from Leuconostoc mesenteroides |
| Specificity: | Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ |
| Specific Activity: | ~ 800 U/mg (30oC, pH 7.8 on glucose-6-phosphate) |
| Unit Definition: | One Unit of Glucose-6-phosphate dehydrogenase activity is defined as the amount of enzyme required to convert one µmole of glucose 6-phosphate (3.3 mM) to 6-phosphogluconate per minute in the presence of NAD+ in Tris-HCl buffer (51 mM), pH 7.8 at 30oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 7.8 |
| Application examples: | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
Symbol :Not ApplicableSignal Word :Not ApplicableHazard Statements :Not ApplicablePrecautionary Statements :Not Applicable
Glucose-6-phosphate dehydrogenase (Escherichia coli)
Description
Documents
Safety Information
Description
| Content: | 5,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.49 |
| CAS Number: | 9001-40-5 |
| Synonyms: | glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase |
| Source: | Escherichia coli |
| Molecular Weight: | 56,770 |
| Concentration: | Supplied at ~ 1,250 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ |
| Specific Activity: | ~ 160 U/mg (25oC, pH 7.6 on D-glucose-6-phosphate) |
| Unit Definition: | One Unit of Glucose-6-phosphate dehydrogenase activity is defined as the amount of enzyme required to convert one µmole of glucose 6-phosphate (5.0 mM) to 6-phosphogluconate per minute in the presence of NADP+ in imidazole buffer (168 mM), pH 7.6 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 7.6 |
| Application examples: | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
Symbol :Not ApplicableSignal Word :Not ApplicableHazard Statements :Not ApplicablePrecautionary Statements :Not Applicable
Hexokinase/Glucose-6-phosphate dehydrogenase
Description
Documents
Safety Information
Description
| Content: | 10 mL Hexokinase (420 U/mL) + G6P-DH (210 U/mL) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Kinase |
| EC Number: | Hexokinase: 2.7.1.1 G6P-DH: 1.1.1.49 |
| CAS Number: | Hexokinase: 9001-51-8 G6P-DH: 9001-40-5 |
| Synonyms: | Hexokinase: Hexokinase; ATP:D-hexose 6-phosphotransferase G6P-DH: glucose-6-phosphate dehydrogenase (NADP+); D-glucose-6-phosphate:NADP+ 1-oxidoreductase |
| Source: | Yeast |
| Concentration: | Hexokinase: ~ 194 U/mL G6P-DH: ~ 665 U/mL |
| Expression: | Hexokinase: Recombinant from yeast G6P-DH: Recombinant from Leuconostoc mesenteroides |
| Specificity: | Hexokinase: Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine. Glucose-6-phosphate dehydrogenase: Catalyses the reaction: D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ |
| Unit Definition: | Hexokinase: One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.5 and 25oC. G6P-DH: One Unit of Glucose-6-phosphate dehydrogenase activity is the amount of enzyme required to convert one μmole of glucose-6-phosphate to 6-phosphogluconate per minute, in the presence of NADP+ at pH 7.8 and 25oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 7.4 |
| Application examples: | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
Symbol :Not ApplicableSignal Word :Not ApplicableHazard Statements :Not ApplicablePrecautionary Statements :Not Applicable
Ammonia Assay Kit (Liquid Ready™
Description
Documents
Safety Information
Description
| Content: | 50 assays (manual) / 500 assays (auto-analyzer) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Analyte: | Ammonia, Nitrogen, YAN |
| Assay Format: | Spectrophotometer, Auto-analyzer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Decrease |
| Linear Range: | 0.4 to 8 µg of ammonia per assay |
| Limit of Detection: | 1 mg/L |
| Limit of Quantification: | 3 mg/L |
| Reproducibility (%): | < 5% |
| Reaction Time (min): | ~ 5 min |
The Ammonia (Liquid Ready™) assay kit is a rapid (~ 5 min), method for the specific measurement and analysis of ammonia in wine, beverages, foodstuffs and other materials. Supplied as a 2-reagent format “ready to use” liquid stable formulation that is suitable for manual and auto-analyzer formats with a simple, reliable and accurate method.
Advantages
- “Ready to use” liquid stable formulation – no reconstitution needed
- Rapid reaction (~ 5 min)
- PVP incorporated to prevent tannin inhibition
- Standard included
- Suitable for manual and auto-analyzer formats
- Quick Reference Guide available
- Mega-Calc™ software tool is available for hassle-free raw data processing
Documents
Safety Information
Symbol :Not ApplicableSignal Word :Not ApplicableHazard Statements :Not ApplicablePrecautionary Statements :Not Applicable
Glutamate pyruvate transaminase (Bacillus subtilis)
Description
Documents
Safety Information
Description
| Content: | 2,500 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Other Activities |
| EC Number: | 2.6.1.21 |
| CAS Number: | 9000-86-6 |
| Synonyms: | D-amino-acid transaminase; D-alanine:2-oxoglutarate aminotransferase |
| Source: | Bacillus subtilis |
| Molecular Weight: | 33,346 |
| Concentration: | Supplied at ~ 2,000 U/mL |
| Expression: | Recombinant from Bacillus Subtilis |
| Specificity: | Catalyses the reaction: D-Alanine + 2 oxoglutarate = pyruvate + D-glutamate |
| Specific Activity: | ~ 40 U/mg (25oC, pH 7.5 on D-alanine) |
| Unit Definition: | One Unit of D-glutamate-pyruvate transaminase is defined as the amount of enzyme required to produce one µmole of D-glutamate per minute from α-ketoglutaric acid (12.4 mM) in the presence of NADH at pH 7.5 and 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 7.5 |
| Application examples: | Applications in diagnostics and analytical methods in the food and feeds, fermentation, beverages and wine industries. |
Documents
Safety Information
Symbol :Not ApplicableSignal Word :Not ApplicableHazard Statements :Not ApplicablePrecautionary Statements :Not Applicable
Hexokinase (yeast)
Description
Documents
Safety Information
Description
| Content: | 10,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Kinase |
| EC Number: | 2.7.1.1 |
| CAS Number: | 9001-51-8 |
| Synonyms: | hexokinase; ATP:D-hexose 6-phosphotransferase |
| Source: | Yeast |
| Molecular Weight: | 53,738 |
| Concentration: | Supplied at ~ 1,280 U/mL |
| Expression: | From Yeast |
| Specificity: | Catalyses the reaction: ATP + D-hexose = ADP + D-hexose 6-phosphate Phopshorylates D-glucose, D-mannose, D-fructose, sorbitol and D-glucosamine. |
| Specific Activity: | ~ 100 U/mg (25oC, pH 7.4 on glucose) |
| Unit Definition: | One Unit of hexokinase activity is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-glucose and glucose-6-phosphate dehydrogenase at pH 7.4 and 25oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 7.6 |
| Application examples: | Applications for the measurement of glucose and other hexoses in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
Symbol :GHS08Signal Word :DangerHazard Statements :H334Precautionary Statements :P261, P284, P304+P340, P342+P311, P501
α-Amylase Reagent (Ceralpha)
Description
Documents
Safety Information
Description
| Content: | 200 assays per kit (4 vials) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Limit of Detection: | 0.05 U/mL |
| Reproducibility (%): | ~ 3% |
| Reaction Time (min): | ~ 30 min |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
myo-Inositol dehydrogenase (Bacillus subtilis)
Description
Documents
Safety Information
Description
| Content: | 500 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.18 |
| CAS Number: | 9028-25-5 |
| Synonyms: | inositol 2-dehydrogenase; myo-Inositol:NAD+ 2-oxidoreductase |
| Source: | Bacillus subtilis |
| Molecular Weight: | 39,417 |
| Concentration: | Supplied at ~ 250 U/mL |
| Expression: | Recombinant from Bacillus Subtilis |
| Specificity: | Catalyses the reaction: myo-inositol + NAD+ = 2,4,6/3,5-pentahydroxycyclohexanone + NADH + H+ |
| Specific Activity: | ~ 50 U/mg (25oC, pH 9.6 on myo-inositol) |
| Unit Definition: | One Unit of myo-inositol dehydrogenase activity is defined as the amount of enzyme required to produce one µmole of scyllo-inosose and NADH from myo-inositol (123 mM) and NAD+ per minute in Glycylglycine buffer (492mM), pH 9.6 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 9.6 |
| Application examples: | Applications for the measurement of myo-inositol acid in the food, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
α-Amylase Assay Kit (Ceralpha Method)
Description
Documents
Safety Information
Description
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.003 U/g |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | ~ 30 min |
| Application examples: | Cereal flours, fermentation broths and other materials. |
| Method recognition: | AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018) |
The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).
Documents
Safety Information
Safety Information
| Symbol : | GHS07 |
| Signal Word : | Warning |
| Hazard Statements : | H315, H319, H335 |
| Precautionary Statements : | P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P337+P313 |
β-Amylase Assay Kit (Betamyl-3)
Description
Documents
Safety Information
Description
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | β-Amylase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.05 U/mL |
| Reproducibility (%): | ~ 3% |
| Reaction Time (min): | ~ 10 min |
| Application examples: | Cereal flours, malts and other materials. |
| Method recognition: | Modification of RACI Standard Method |
The Betamyl-3; β-Amylase test kit is suitable for the specific measurement and analysis of β-amylase in malt flour.
Advantages
- Very cost effective
- All reagents stable for > 2 years as supplied
- Only enzymatic kit available
- Very specific
- Simple format
- Rapid reaction
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
Documents
Safety Information
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P261, P284, P304+P340, P342+P311, P501 |
Papain (Carica papaya)
Description
Documents
Safety Information
Description
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.003 U/g |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | ~ 30 min |
| Application examples: | Cereal flours, fermentation broths and other materials. |
| Method recognition: | AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018) |
The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).
Advantages
- Very cost effective
- All reagents stable for > 2 years after preparation
- Very specific
- Simple format
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
Validation of Methods
Documents
Safety Information
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P261, P284, P304+P340, P342+P311, P501 |
Phosphatase (Acid) (Escherichia coli)
Description
Documents
Safety Information
Description
| Content: | 400 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate containing 1 mM MgCl2 and 0.1 mM ZnSO4 |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Phosphatase |
| EC Number: | 3.1.3.2 |
| CAS Number: | 9001-77-8 |
| Synonyms: | acid phosphatase; phosphate-monoester phosphohydrolase (acid optimum); orthophosphoric-monoester phosphohydrolase (acid optimum) |
| Source: | Escherichia coli |
| Molecular Weight: | 24,667 |
| Concentration: | Supplied at ~ 200 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Catalyses the reaction: A phosphate monoester + H2O = an alcohol + phosphate |
| Specific Activity: | ~ 14 U/mg (40oC, pH 5.0 on 4-Nitrophenyl phosphate) |
| Unit Definition: | One Unit of acid phosphatase is defined as the amount of enzyme required to release one µmole of p-nitrophenol per minute from 4-nitrophenyl phosphate (4 mM) in sodium acetate buffer (20 mM), pH 5.0 at 40oC. |
| Temperature Optima: | 45oC |
| pH Optima: | 5 |
| Application examples: | Applications in research including molecular biology and biocatalysis. |
Documents
Safety Information
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P284, P304+P340, P342+P311, P501 |
D-Lactate dehydrogenase (Leuconostoc mesenteroides)
Description
Documents
Safety Information
Description
| Content: | 22,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.28 |
| CAS Number: | 9028-36-8 |
| Synonyms: | D-lactate dehydrogenase; (R)-lactate:NAD+ oxidoreductase |
| Source: | Leuconostoc mesenteroides |
| Molecular Weight: | 36,455 |
| Concentration: | Supplied at ~ 20,000 U/mL |
| Expression: | Recombinant from Leuconostoc mesenteroides |
| Specificity: | Catalyses the reaction: D-Lactate + NAD+ = pyruvate + NADH + H+ |
| Specific Activity: | ~ 1,500 U/mg (25oC, pH 7.0 on pyruvic acid) |
| Unit Definition: | One Unit of D-lactate dehydrogenase is defined as the amount of enzyme required to produce one µmole of D-lactate from pyruvic acid (0.77 mM) per minute in the presence of NADH in sodium phosphate buffer (92 mM), pH 7.0 at 25oC. |
| Temperature Optima: | 37oC |
| pH Optima: | 7 |
| Application examples: | Applications for the measurement of D-lactic acid in the food, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
L-Lactate dehydrogenase (Porcine)
Description
Documents
Safety Information
Description
| Content: | 6,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.27 |
| CAS Number: | 9001-60-9 |
| Synonyms: | L-lactate dehydrogenase; (S)-lactate:NAD+ oxidoreductase |
| Source: | Porcine |
| Molecular Weight: | 36,000 |
| Concentration: | Supplied at ~ 2,000 U/mL |
| Expression: | Recombinant from Porcine origin |
| Specificity: | Catalyses the reaction: L-Lactate + NAD+ = pyruvate + NADH + H+ |
| Specific Activity: | ~ 330 U/mg (37oC, pH 7.0 on pyruvic acid) |
| Unit Definition: | One Unit of L-lactate dehydrogenase activity is defined as the amount of enzyme required to produce one µmole of NAD+ from NADH in the presence of pyruvic acid (0.8mM) in sodium phosphate buffer (100mM), pH 7.0 at 37oC. |
| Temperature Optima: | 37oC |
| pH Optima: | 7 |
| Application examples: | Applications for the measurement of L-lactic acid in the food, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Glutamate oxaloacetate transaminase (Escherichia coli)
Description
Documents
Safety Information
Description
| Content: | 5,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Other Activities |
| EC Number: | 2.6.1.1 |
| CAS Number: | 9000-97-9 |
| Synonyms: | aspartate transaminase; L-aspartate:2-oxoglutarate aminotransferase |
| Source: | Escherichia coli |
| Molecular Weight: | 45,737 |
| Concentration: | Supplied at ~ 2,500 U/mL |
| Expression: | Recombinant from Escherichia coli |
| Specificity: | Catalyses the reversible transfer of an α-amino group between aspartate and glutamate. |
| Specific Activity: | ~ 200 U/mg (25oC, pH 8.5 on α-ketoglutaric acid) |
| Unit Definition: | One Unit of L-glutamic-oxaloacetic transaminase activity is defined as the amount of enzyme required to convert one µmole of α-ketoglutarate to L-glutamate per minute in the presence of NADH at pH 8.5 and 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 8.5 |
| Application examples: | Applications in diagnostics and analytical methods in the food and feeds, fermentation, beverages and wine industries. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Protease (Subtilisin A from Bacillus licheniformis)
Description
Documents
Safety Information
Description
| Content: | 0.5 grams – 10 mL or 2 grams – 40 mL or 5 grams – 100 mL or 2.5 grams – 100 mL (ANKOM) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Protease |
| EC Number: | 3.4.21.62 |
| CAS Number: | 9014-01-1 |
| Synonyms: | subtilisin; subtilisin A |
| Source: | Bacillus licheniformis |
| Molecular Weight: | 30,250 |
| Expression: | Purified from Bacillus licheniformis |
| Specificity: | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides. |
| Specific Activity: | ~ 6 U/mg of protein (40oC, pH 8.0 on casein) |
| Unit Definition: | One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 µg) of tyrosine per minute at pH 7.0 at 40oC (colour by Folin-Ciocalteu reagent). |
| Temperature Optima: | 60oC |
| pH Optima: | 7 |
| Application examples: | This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fiber analytical procedures. |
Documents
Safety Information
| Symbol : | GHS05, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H318, H334 |
| Precautionary Statements : | P261, P280, P284, P304+P340, P305+P351+P338, P310 |
Protease Subtilisin A (from Bacillus licheniformis) Powder
Description
Documents
Safety Information
Description
| Content: | 1 gram, powder |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | In powder form |
| Physical Form: | Powder |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Protease |
| EC Number: | 3.4.21.62 |
| CAS Number: | 9014-01-1 |
| Synonyms: | subtilisin; subtilisin A |
| Source: | Bacillus licheniformis |
| Molecular Weight: | 30,250 |
| Expression: | Purified from Bacillus licheniformis |
| Specificity: | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides. |
| Specific Activity: | > 8 U/mg of protein (40oC, pH 8.0 on casein) |
| Unit Definition: | One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 µg) of tyrosine per minute at pH 7.0 at 40oC (colour by Folin-Ciocalteu reagent). |
| Temperature Optima: | 60oC |
| pH Optima: | 7 |
| Application examples: | This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fiber analytical procedures. |
High purity Protease (Subtilisin A from Bacillus licheniformis) (Powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
.
Documents
Safety Information
| Symbol : | GHS05, GHS07, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H315, H318, H334, H335 |
| Precautionary Statements : | P261, P264, P271, P280, P284, P302+P352 |
Uridylate kinase (prokaryote)
Description
Documents
Safety Information
Description
| Content: | 150 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Kinase |
| EC Number: | 2.7.4.22 |
| CAS Number: | 9036-23-1 |
| Synonyms: | ATP:UMP phosphotransferase; UMP-kinase; uridine monophosphate kinase |
| Source: | Prokaryote |
| Molecular Weight: | 26,000 |
| Concentration: | Supplied at ~ 700 U/mL |
| Expression: | Recombinant from a Prokaryote |
| Specificity: | Catalyses the reaction: ATP + UMP = ADP + UDP Catalyses the ATP-dependent phosphorylation of Uridine monophosphate(UMP) into Uridine diphosphate (UDP). |
| Specific Activity: | ~ 24 U/mg (25oC, pH 7.6 on UMP) |
| Unit Definition: | One unit of activity is defined as the amount of enzyme required to form one μmole of UDP from UMP and ATP at pH 7.6 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 7.6 |
| Application examples: | Applications in the measurement of UMP. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Phosphoglucose isomerase (Bacillus subtilis)
Description
Documents
Safety Information
Description
| Content: | 5,000 Units or 50,000 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Isomerase |
| EC Number: | 5.3.1.9 |
| CAS Number: | 9001-41-6 |
| Synonyms: | glucose-6-phosphate isomerise; D-glucose-6-phosphate aldose-ketose-isomerase |
| Source: | Bacillus subtilis |
| Molecular Weight: | 50,500 |
| Concentration: | E-PGIBS-5KU: Supplied at > 1,000 U/mL. E-PGIBS-50KU: Supplied at ~ 3,550 U/mL. |
| Expression: | Recombinant from Bacillus Subtilis |
| Specificity: | Catalyses the reaction: D-Glucose 6-phosphate = D-fructose 6-phosphate |
| Specific Activity: | > 60 U/mg of protein (25oC, pH 7.6 on fructose 6-phosphate); > 120 U/mg (40oC, pH 7.6 on fructose 6-phosphate) |
| Unit Definition: | One unit of phosphoglucose isomerase activity is the amount of enzyme required to convert one µmole of D-fructose 6-phosphate to D-glucose 6-phosphate at pH 7.6. |
| Temperature Optima: | 40oC |
| pH Optima: | 7.6 |
| Application examples: | Applications for the measurement of D-fructose in carbohydrate research and in the food and feeds, fermentation, wine, beverage and dairy industries. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Glucomannan Assay Kit
Description
Documents
Safety Information
Description
| Content: | 50 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Glucomannan |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 4 to 80 μg of glucomannan per assay |
| Limit of Detection: | 1 g/100 g |
| Total Assay Time: | 120 min |
| Application examples: | Jelly sweets, cosmetics, food gums and other materials. |
| Method recognition: | Novel method |
Documents
Safety Information
| Symbol : | GHS07 |
| Signal Word : | Warning |
| Hazard Statements : | H319 |
| Precautionary Statements : | P264, P280, P305+P351+P338, P337+P313 |
Succinyl-CoA synthetase
Description
Documents
Safety Information
Description
| Content: | 550 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Other Activities |
| EC Number: | 6.2.1.5 |
| CAS Number: | 9080-33-5 |
| Synonyms: | succinate-CoA ligase (ADP-forming); succinate:CoA ligase (ADP-forming) |
| Source: | Prokaryote |
| Molecular Weight: | 30,843, 41,393 |
| Concentration: | Supplied at ~ 220 U/mL |
| Expression: | Recombinant from a Prokaryotic source |
| Specificity: | Catalyses the reaction: ATP + succinate + CoA = ADP + phosphate + succinyl-CoA |
| Specific Activity: | ~ 9 U/mg (25oC, pH 8.4 on succinic acid) |
| Unit Definition: | One Unit of Succinyl-CoA synthetase activity is defined as the amount of enzyme required to release one µmole of succinyl CoA from succinic acid (5.8 mM) per minute in the presence of NADH and Coenzyme A in Glycylglycine buffer (34 mM), pH 8.4 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 8.4 |
| Application examples: | Applications for the measurement of succinic acid in the clinical chemistry, food, wine and dairy industries. |
High purity recombinant Succinyl-CoA synthetase (prokaryote) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Xylose dehydrogenase + Xylose mutarotase
Description
Documents
Safety Information
Description
| Content: | 150 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Kinase |
| EC Number: | 2.7.4.22 |
| CAS Number: | 9036-23-1 |
| Synonyms: | ATP:UMP phosphotransferase; UMP-kinase; uridine monophosphate kinase |
| Source: | Prokaryote |
| Molecular Weight: | 26,000 |
| Concentration: | Supplied at ~ 700 U/mL |
| Expression: | Recombinant from a Prokaryote |
| Specificity: | Catalyses the reaction: ATP + UMP = ADP + UDP Catalyses the ATP-dependent phosphorylation of Uridine monophosphate(UMP) into Uridine diphosphate (UDP). |
| Specific Activity: | ~ 24 U/mg (25oC, pH 7.6 on UMP) |
| Unit Definition: | One unit of activity is defined as the amount of enzyme required to form one μmole of UDP from UMP and ATP at pH 7.6 at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 7.6 |
| Application examples: | Applications in the measurement of UMP. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
β-Glucan Assay Kit (Mixed Linkage
Description
Documents
Safety Information
Description
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | β-Glucan |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 510 |
| Signal Response: | Increase |
| Linear Range: | 4 to 100 μg of D-glucose per assay |
| Limit of Detection: | 0.5 g/100 g |
| Total Assay Time: | ~ 100 min |
| Application examples: | Oats, barley, malt, wort, beer, food and other materials. |
| Method recognition: | AACC Method 32-23.01, AOAC Method 995.16, AOAC Method 992.28, CODEX Method Type II, EBC Method 3.10.1, ICC Standard No. 166 and RACI Standard Method |
The Beta-Glucan test kit is suitable for the measurement and analysis of Beta-Glucan (Mixed Linkage).
For the measurement of 1,3:1,4-β-D-glucan in cereal grains, milling fractions, wort, beer and other food products.
Documents
Safety Information
| Symbol : | GHS05, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H314, H315, H319, H334 |
| Precautionary Statements : | P260, P261, P264, P280, P284, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340 |
Mannitol dehydrogenase (Pseudomona fluorescens)
Description
Documents
Safety Information
Description
| Content: | 500 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | > 4 years at 4oC |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | 1.1.1.67 |
| CAS Number: | 9001-65-4 |
| Synonyms: | mannitol 2-dehydrogenase; D-mannitol:NAD+ 2-oxidoreductase |
| Source: | Pseudomona fluorescens |
| Molecular Weight: | 56,661 |
| Concentration: | Supplied at ~ 300 U/mL |
| Expression: | Recombinant from Pseudomona fluorescens |
| Specificity: | Catalyses the reaction: D-Mannitol + NAD+ = D-fructose + NADH + H+ |
| Specific Activity: | ~ 50 U/mg (40oC, pH 8.6 on D-mannitol) |
| Unit Definition: | One Unit of mannitol dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ in the presence of D-mannitol (5.2 mM) in Tris-HCl buffer (64 mM), pH 8.6 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 8.6 |
| Application examples: | Low Km, suitable for D-mannitol determination. Potential uses in the biotechnology industry. |
Documents
Safety Information
| Symbol : | GHS05, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H314, H315, H319, H334 |
| Precautionary Statements : | P260, P261, P264, P280, P284, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340 |
Carrez Clarification Kit
Description
Documents
Safety Information
Description
| Content: | 40-1000 assays per kit (depending on application) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: Ambient, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Reaction Time (min): | ~ 15 min |
| pH Range: | 7.5-8 (recommended) |
| Application examples: | Dairy samples including milk, cream, whey protein, cheese, yoghurt and infant formula. Other foodstuffs such as meat, fish may also be possible. |
The Carrez Clarification Kit provides easy and ready to use reagents for the removal of interfering compounds such as proteins and fats from samples, allowing improved handling and more accurate measurement of target analytes such as carbohydrates and alcohols.
Note: this method is unsuitable for sample preparation prior to the determination of ascorbic acid (vitamin C), citrate and urea/ammonia.
Documents
Safety Information
| Symbol : | GHS05, GHS09 |
| Signal Word : | Danger |
| Hazard Statements : | H318, H410 |
| Precautionary Statements : | P273, P280, P305+P351+P338, P310, P391 |
β-Glucan Assay Kit (Mixed Linkage
Description
Documents
Safety Information
Description
| Content: | 100 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | β-Glucan |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 510 |
| Signal Response: | Increase |
| Linear Range: | 4 to 100 μg of D-glucose per assay |
| Limit of Detection: | 0.5 g/100 g |
| Total Assay Time: | ~ 100 min |
| Application examples: | Oats, barley, malt, wort, beer, food and other materials. |
| Method recognition: | AACC Method 32-23.01, AOAC Method 995.16, AOAC Method 992.28, CODEX Method Type II, EBC Method 3.10.1, ICC Standard No. 166 and RACI Standard Method |
The Beta-Glucan test kit is suitable for the measurement and analysis of Beta-Glucan (Mixed Linkage).
For the measurement of 1,3:1,4-β-D-glucan in cereal grains, milling fractions, wort, beer and other food products.
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Safety Information
| Symbol : | GHS05, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H314, H315, H319, H334 |
| Precautionary Statements : | P260, P261, P264, P280, P284, P301+P330+P331, P302+P352, P303+P361+P353, P304+P340 |
Total Dietary Fiber Controls
Description
Documents
Safety Information
Description
| Content: | Contains 6 Controls: Use with K-TDFR, K-INTDF or K-RINTDF |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: Ambient, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Dietary Fiber |
For use with the Total Dietary Fiber Assay Kit. Contains barley β-glucan, high amylose maize starch, wheat starch, casein, pectin and larch galactan. Wheat arabinoxylan is available on request.
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Xylose dehydrogenase + Xylose mutarotase
Description
Documents
Safety Information
Description
| 2.5 mL; xylose dehydrogenase (60 U/mL) / xylose mutarotase (2 mg/mL) | |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 2 years below -10oC |
| Enzyme Activity: | Dehydrogenase |
| EC Number: | XDH: 1.1.1.175 XMR: 5.1.3.3 |
| CAS Number: | XDH: 62931-20-8 XMR: 9031-76-9 |
| Synonyms: | XDH: D-xylose 1-dehydrogenase; D-xylose:NAD+ 1-oxidoreductase XMR: Aldose 1-epimerase |
| Molecular Weight: | 26,000 |
| Concentration: | Supplied at ~ 60 U/mL |
| Expression: | Recombinant |
| Specificity: | Interconversion of the α- and β-anomeric forms of D-xylose is catalysed by xylose mutarotase (XMR) (1). (1) α-D-Xylose ↔ β-D-xylose. The β-D-xylose is oxidised by NAD+ to D-xylonic acid in the presence of β-xylose dehydrogenase (β-XDH) at pH 7.5 (2). (2) β-D-Xylose + NAD+ ↔ D-xylonic acid + NADH + H+ |
| Specific Activity: | XDH: ~ 60 U/mL at pH 7.5 and 25oC / XMR: 2 mg/mL |
| Unit Definition: | One Unit of xylose dehydrogenase is defined as the amount of enzyme required to produce one µmole of NADH from NAD+ per minute at 25oC. |
| Temperature Optima: | 25oC |
| pH Optima: | 7.5 |
| Application examples: | For the measurement of D-xylose, especially in broths and hydrolysates of plant material and polysaccharides. Refer to the D-XYLOSE |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Malt β-Glucanase/Lichenase Assay Kit (MBG4 Method)
Description
Documents
Safety Information
Description
| Content: | (Malt β-glucanase) 100 assays (manual) / 400 (auto-analyser) Or (Lichenase) 100 / 200 assays (manual) / 330 (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | β-Glucanase/Lichenase |
| Assay Format: | Spectrophotometer, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | (Malt β-glucanase) 4.3 x 10-4 U/mL Or (Lichenase) 9.1 x 10-5 U/mL |
| Reproducibility (%): | ~ 3% |
| Total Assay Time: | (Malt β-glucanase) ~ 20 min Or (Lichenase) ~ 10 min |
| Application examples: | Crude malt extracts, industrial enzyme preparations. |
| Method recognition: | Novel method |
The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.
Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).
Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
endo-1,5-α-Arabinanase (Aspergillus niger)
Description
Documents
Safety Information
Description
| Content: | 400 Units |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 3.2 M ammonium sulphate |
| Physical Form: | Suspension |
| Stability: | Minimum 1 year at 4oC. Check vial for details. |
| Enzyme Activity: | endo-Arabinanase |
| EC Number: | 3.2.1.99 |
| CAZy Family: | GH43 |
| CAS Number: | 75432-96-1 |
| Synonyms: | arabinan endo-1,5-alpha-L-arabinanase; 5-alpha-L-arabinan 5-alpha-L-arabinanohydrolase |
| Source: | Aspergillus niger |
| Molecular Weight: | 39,750 |
| Concentration: | Supplied at ~ 200 U/mL |
| Expression: | From Aspergillus niger |
| Specificity: | endo-hydrolysis of (1,5)-α-arabinofuranose linkages in (1,5)-α-arabinans. Acts more slowly on the (1,5)-α-linked arabinan backbone in branched arabinans than on linear arabinans. |
| Specific Activity: | ~ 10 U/mg (40oC, pH 4.0 on CM-linear 1,5-α-L-arabinan) |
| Unit Definition: | One Unit of arabinanase activity is defined as the amount of enzyme required to release one µmole of arabinose reducing-sugar equivalents per minute from CM-linear 1,5-α-L-arabinan (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40oC. |
| Temperature Optima: | 40oC |
| pH Optima: | 4 |
| Application examples: | Applications established in food and beverage industries, particularly in the reduction of haze in fruit juices and the processing of sugar beet. |
Documents
Safety Information
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
