Analytical Enzymes
Total Dietary Fiber Assay Kit
| Content: | 100 assays / 200 assays |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Dietary Fiber |
| Assay Format: | Enzymatic |
| Detection Method: | Gravimetric |
| Signal Response: | Increase |
| Limit of Detection: | 0.5 g/100 g |
| Total Assay Time: | ~ 100 min |
| Application examples: | Food ingredients, food products and other materials. |
| Method recognition: | AACC Method 32-05.01, AACC Method 32-06.01, AACC Method 32-07.01, AACC Method 32-21.01, AOAC Method 985.29, AOAC Method 991.42, AOAC Method 991.43, AOAC Method 993.19, CODEX Method Type I and GB Standard 5009.88-2014 |
Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits.
Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al.1 and Prosky et al.2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.
1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.
2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.
3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.
See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998.
Two separate methods are described in the associated assay protocol:
METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER
Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).
METHOD 2: DETERMINATION OF TOTAL DIETARY FIBER
Based on AACC method 32-05.01 and AOAC Method 985.29.
Note that a letter of endorsement from the original method developer, Dr. Leon Prosky, is included in the Documents Tab.
- Very competitive price (cost per test)
- All reagents stable for > 2 years
- High purity / standardised enzymes employed
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Simple format
| Symbol : | GHS05, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H318, H334 |
| Precautionary Statements : | P261, P280, P284, P304+P340, P305+P351+P338, P310, P342+P311, P501 |
L-Arginine/Urea/Ammonia Assay Kit
| Content: | 50 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 1 year under recommended storage conditions |
| Analyte: | Ammonia, L-Arginine, Nitrogen, Urea, YAN |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Decrease |
| Linear Range: | 1.0 to 35 mg of L-arginine, 0.2 to 7.0 μg of ammonia and 0.3 to 14 µg of urea per assay |
| Limit of Detection: | 0.07 mg/L (ammonia), 0.13 mg/L (urea), 0.37 mg/L (L-arginine) |
| Reaction Time (min): | ~ 20 min [ammonia (2 min), urea (6 min), L-arginine (7 min)] |
| Application examples: | Grape juice, wine must, wine and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Improved method |
The L-Arginine/Urea/Ammonia test kit is specific and a rapid measurement and analysis of L-arginine, urea and ammonia in grape juice/must and wine.
Symbol :GHS07, GHS08Signal Word :DangerHazard Statements :H302, H315, H319, H360Precautionary Statements :P201, P202, P264, P270, P280, P301+P312, P302+P352, P305+P351+P338, P330, P337+P313, P501
Fructan HK Assay Kit
| Content: | 50 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Fructan |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 4 to 80 µg of D-glucose, D-fructose or sucrose per assay |
| Limit of Detection: | 1 g/100 g |
| Total Assay Time: | ~ 30 min |
| Application examples: | Flours, plant materials (e.g. onion), food products and other materials |
The Fructan HK test kit is suitable for the specific measurement and analysis of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose.
| Symbol : | GHS05, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H314, H360 |
| Precautionary Statements : | P201, P202, P260, P264, P280 |
Lactose Assay Kit - Sequential/High Sensitivity
| Content: | 65 assays per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Lactose |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 1 to 50 µg of lactose (or 0.50 to 25 µg of D-glucose) |
| Limit of Detection: | 1.62 mg/L |
| Reaction Time (min): | ~ 28 min |
| Method recognition: | AOAC Method 2020.08 |
The K-LOLAC test kit offers a rapid, novel, sequential measurement of free-glucose and lactose in conventional, low-lactose and lactose-free dairy products. This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Megazyme Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products. This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase. Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.
| Symbol : | GHS05, GHS07, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H302, H302+H332, H314, H334, H360 |
| Precautionary Statements : | P201, P202, P260, P261, P264, P270, P271, P280, P284, P301+P312, P301+P330+P331, P304+P340, P342+P311, P501 |
Primary Amino Nitrogen Assay Kit (PANOPA)
| Content: | 100 assays (manual) / 1000 assays (microplate) / 1100 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | Nitrogen, Primary Amino Nitrogen, YAN |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.2 to 10 µg of amino nitrogen per assay |
| Limit of Detection: | 2.59 mg N/L |
| Reaction Time (min): | ~ 15 min |
| Application examples: | Grape juice, must, wine and other materials. |
| Method recognition: | Novel method |
The Primary Amino Nitrogen (PANOPA) Assay Kit is suitable for the measurement and analysis of primary amino nitrogen in grape juice/must and wine.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave
| Symbol : | GHS05, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H314, H360 |
| Precautionary Statements : | P201, P202, P260, P264, P280 |
Sucrase (from yeast)
| Content: | 300 Units (on sucrose) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Formulation: | Supplied as a lyophilised powder |
| Physical Form: | Powder |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Sucrase/Invertase |
| EC Number: | 3.2.1.20 |
| CAZy Family: | GH13 |
| CAS Number: | 9001-42-7 |
| Synonyms: | alpha-glucosidase; alpha-D-glucoside glucohydrolase |
| Source: | Yeast |
| Molecular Weight: | 62,000 |
| Expression: | Purified from Yeast |
| Specificity: | Hydrolysis of terminal, non-reducing (1,4)-linked α-D-glucose residues with release of D-glucose. |
| Specific Activity: | ~ 20 U/mg (30oC, pH 6.8 on sucrose) |
| Unit Definition: | One Unit of sucrase activity is defined as the amount of enzyme required to release one µmole of glucose per minute from sucrose (10 mM) in sodium maleate buffer (100 mM), pH 6.8 at 30oC. |
| Temperature Optima: | 30oC |
| pH Optima: | 6.8 |
| Application examples: | Applications for the removal of sucrose in various analytical procedures in the cereals, food and feeds, fermentation and beverage industries. |
| Method recognition: | AOAC Method 2016.06 and GB Standard 5009.255-2016 |
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P261, P284, P304+P340, P342+P311, P501 |
α-Amylase (Bacillus licheniformis)
| Enzyme Activity: | α-Amylase |
| EC Number: | 3.2.1.1 |
| CAZy Family: | GH13 |
| CAS Number: | 9000-90-2, 9000-85-5 |
| Synonyms: | alpha-amylase; 4-alpha-D-glucan glucanohydrolase |
| Source: | Bacillus licheniformis |
| Molecular Weight: | 58,000 |
| Expression: | Purified from Bacillus licheniformis |
| Specificity: | endo-hydrolysis of α-1,4-D-glucosidic linkages in starch. |
| Specific Activity: | ~ 55 U/mg (40oC, pH 6.5 on Ceralpha reagent) |
| Unit Definition: | One Unit of α-amylase is the amount of enzyme required to release one µmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.5 and 40oC. |
| Temperature Optima: | 75oC |
| pH Optima: | 6.5 |
| Application examples: | For use in Megazyme Dietary Fiber methods. |
| Method recognition: | EBC Method 6.5 |
High purity α-Amylase (Bacillus licheniformis) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Dietary Fiber methods, suitable for use at pH 6.5 and above.
E-BLAAM-A-100mL specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P261, P284, P304+P340, P342+P311, P501 |
Amyloglucosidase (Aspergillus niger)
| Content: | 10 mL or 40 mL or 100 mL or 100 mL (ANKOM) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Amyloglucosidase |
| EC Number: | 3.2.1.3 |
| CAZy Family: | GH15 |
| CAS Number: | 9032-08-0 |
| Synonyms: | glucan 1,4-alpha-glucosidase; 4-alpha-D-glucan glucohydrolase; glucoamylase |
| Source: | Aspergillus niger |
| Molecular Weight: | 143,500 |
| Expression: | From Aspergillus niger |
| Specificity: | Hydrolysis of terminal α-1,4 and α-1,6 D-glucose residues successively from non-reducing ends of maltodextrins. |
| Specific Activity: | ~ 3,260 U/mL (40oC, pH 4.5 on soluble starch) ~ 200 U/mL (40oC, pH 4.5 on p-nitrophenyl β-maltoside) |
| Unit Definition: | One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of D-glucose reducing-sugar equivalents per minute from soluble starch at pH 4.5 and 40oC. |
| Temperature Optima: | 70oC |
| pH Optima: | 4 |
| Application examples: | Applications for the measurement of starch and dietary fiber in the food and feeds industry. |
| Method recognition: | AOAC Method 2000.11 and GB Standard 5009.245-2016 |
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P261, P284, P304+P340, P342+P311, P501 |
Protease (Subtilisin A from Bacillus licheniformis)
| Content: | 0.5 grams – 10 mL or 2 grams – 40 mL or 5 grams – 100 mL or 2.5 grams – 100 mL (ANKOM) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Formulation: | In 50% (v/v) glycerol |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | Protease |
| EC Number: | 3.4.21.62 |
| CAS Number: | 9014-01-1 |
| Synonyms: | subtilisin; subtilisin A |
| Source: | Bacillus licheniformis |
| Molecular Weight: | 30,250 |
| Expression: | Purified from Bacillus licheniformis |
| Specificity: | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides. |
| Specific Activity: | ~ 6 U/mg of protein (40oC, pH 8.0 on casein) |
| Unit Definition: | One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 µg) of tyrosine per minute at pH 7.0 at 40oC (colour by Folin-Ciocalteu reagent). |
| Temperature Optima: | 60oC |
| pH Optima: | 7 |
| Application examples: | This enzyme is recommended for use in the Megazyme Total Dietary Fiber test method and AOAC INTERNATIONAL Total Dietary Fiber analytical procedures. |
High purity Protease (Subtilisin A from Bacillus licheniformis) (liquid) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
For use in Megazyme Total Dietary Fiber test method.
E-BSPRT-A-100ML specifically to be used with ANKOMTDF Dietary Fiber Analyzer.
| Symbol : | GHS05, GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H318, H334 |
| Precautionary Statements : | P261, P280, P284, P304+P340, P305+P351+P338, P310 |
α-Amylase (Thermostable) (Bacillus sp.)
| Content: | 10 mL; 3,000 U/mL |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 4oC |
| Formulation: | In 50% (v/v) glycerol plus 0.02% sodium azide |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | α-Amylase |
| EC Number: | 3.2.1.1 |
| CAZy Family: | GH13 |
| CAS Number: | 9000-90-2, 9000-85-5 |
| Synonyms: | alpha-amylase; 4-alpha-D-glucan glucanohydrolase |
| Source: | Bacillus sp. |
| Molecular Weight: | 58,000 |
| Concentration: | Supplied at ~ 3,000 U/mL |
| Expression: | Purified from Bacillus sp. |
| Specificity: | Hydrolysis of α-1,4 glucosidic linkages in linear α-1,4 glucan (e.g. amylose regions in starch). |
| Specific Activity: | ~ 170 U/mg (40oC, pH 6.5 on Ceralpha reagent) |
| Unit Definition: | One Unit of α-amylase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.5 and 40oC. |
| Temperature Optima: | 100oC |
| pH Optima: | 7 |
| Application examples: | For use in carbohydrate research. |
High purity α-Amylase (Thermostable) (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This enzyme is considerably more thermostable than related enzymes such as the heat stable α-amylase (Bacillus
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P261, P284, P304+P340, P342+P311, P501 |
Red Debranched Arabinan (Sugar Beet)
| Content: | 2 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Physical Form: | Powder |
| Stability: | > 6 months under recommended storage conditions |
| Substrate For (Enzyme): | endo-Arabinanase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 520 |
| Reproducibility (%): | ~ 7% |
This product has been discontinued.
High purity dyed, soluble Red Debranched Arabinan (Sugar Beet) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Debranched arabinan dyed with Procion Red dye. Substrate for the assay of endo-1,5-α-L-arabinanase.
Please note the video above shows the protocol for assay of endo-cellulase using Azo-CM cellulose. The procedure for the assay of endo-arabinanase using Red Debranched Arabinan (Sugar Beet) is equivalent to this
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Red Starch
| Content: | 10 mL; 3,000 U/mL |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 4oC |
| Formulation: | In 50% (v/v) glycerol plus 0.02% sodium azide |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | α-Amylase |
| EC Number: | 3.2.1.1 |
| CAZy Family: | GH13 |
| CAS Number: | 9000-90-2, 9000-85-5 |
| Synonyms: | alpha-amylase; 4-alpha-D-glucan glucanohydrolase |
| Source: | Bacillus sp. |
| Molecular Weight: | 58,000 |
| Concentration: | Supplied at ~ 3,000 U/mL |
| Expression: | Purified from Bacillus sp. |
| Specificity: | Hydrolysis of α-1,4 glucosidic linkages in linear α-1,4 glucan (e.g. amylose regions in starch). |
| Specific Activity: | ~ 170 U/mg (40oC, pH 6.5 on Ceralpha reagent) |
| Unit Definition: | One Unit of α-amylase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.5 and 40oC. |
| Temperature Optima: | 100oC |
| pH Optima: | 7 |
| Application examples: | For use in carbohydrate research. |
High purity α-Amylase (Thermostable) (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This enzyme is considerably more thermostable than related enzymes such as the heat stable α-amylase (Bacillus
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P261, P284, P304+P340, P342+P311, P501 |
Azo-CM-Cellulose (Liquid)
| Content: | 10 mL; 3,000 U/mL |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 4oC |
| Formulation: | In 50% (v/v) glycerol plus 0.02% sodium azide |
| Physical Form: | Solution |
| Stability: | > 1 year under recommended storage conditions |
| Enzyme Activity: | α-Amylase |
| EC Number: | 3.2.1.1 |
| CAZy Family: | GH13 |
| CAS Number: | 9000-90-2, 9000-85-5 |
| Synonyms: | alpha-amylase; 4-alpha-D-glucan glucanohydrolase |
| Source: | Bacillus sp. |
| Molecular Weight: | 58,000 |
| Concentration: | Supplied at ~ 3,000 U/mL |
| Expression: | Purified from Bacillus sp. |
| Specificity: | Hydrolysis of α-1,4 glucosidic linkages in linear α-1,4 glucan (e.g. amylose regions in starch). |
| Specific Activity: | ~ 170 U/mg (40oC, pH 6.5 on Ceralpha reagent) |
| Unit Definition: | One Unit of α-amylase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.5 and 40oC. |
| Temperature Optima: | 100oC |
| pH Optima: | 7 |
| Application examples: | For use in carbohydrate research. |
High purity α-Amylase (Thermostable) (Bacillus sp.) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. This enzyme is considerably more thermostable than related enzymes such as the heat stable α-amylase (Bacillus
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P261, P284, P304+P340, P342+P311, P501 |
Azo-CM-Cellulose (Powder)
| Content: | 4 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Cellulase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azo-CM-Cellulose for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Substrate for the specific measurement of endo-1,4-β-D-glucanase (cellulase).
Please note the video above shows the protocol for assay of endo-cellulase using Azo-CM cellulose. The procedure for the assay of endo-Cellulase using Azo-CM-Cellulose (Powder) is equivalent to this.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azo-Xyloglucan (Tamarind)
| Content: | 4 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 8 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Cellulase, Xyloglucanase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
This product has been discontinued
High purity dyed, soluble Azo-Xyloglucan (Tamarind) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
A soluble chromogenic substrate for the assay of endo-cellulase.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azo-Barley Glucan
| Content: | 100 mL (1% w/v) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Physical Form: | Liquid |
| Stability: | > 1 year under recommended storage conditions |
| Substrate For (Enzyme): | endo-Cellulase, β-Glucanase/Lichenase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azo-Barley Glucan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Highly purified, low viscosity barley 1,3:1,4-β-D-glucan dyed with Remazol Brilliant Blue R dye. Recommended substrate for the measurement of β-glucanase in malt flour.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azo-Fructan
| Content: | 5 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 9 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Inulinase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azo-Fructan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Substrate for the measurement of endo-inulinase
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azo-Galactan (Potato)
| Content: | 4 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 12 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-1,4-β-Galactanase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azo-Galactan (Potato) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Substrate for the specific measurement of endo-1,4-β-D-galactanase. Prepared from potato galactan pre-treated with α-L-arabinofuranosidase to reduce arabinose content.
Please note the video above shows the protocol for assay of endo-cellulase using Azo-CM cellulose. The procedure for the assay of endo-1,4-β-galactanase using Azo-Galactan (Potato) is equivalent to this.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Red Pullulan
| Content: | 3 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | Isoamylase, Isopullulanase, Neopullulanase, Pullulanase/Limit-Dextrinase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 510 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Red Pullulan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Substrate for measurement of pullulanase in commercial enzyme preparations and for the measurement of limit-dextrinase in malt flours.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azo-Carob Galactomannan
| Content: | 4 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-1,4-β-Mannanase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azo-Carob Galactomannan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Substrate for the specific measurement of endo-1,4-β-D-mannanase.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azocasein
| Content: | 10 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | Protease |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 440 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azocasein (Sulphanilamide Dyed) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
A highly sensitive, soluble substrate for the assay of endo-protease activity. This substrate has a 5-fold greater sensitivity than similar products supplied by other companies.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
AZ-Rhamnogalacturonan
| Content: | 2 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 9 years under recommended storage conditions |
| Substrate For (Enzyme): | Rhamnogalacturonan Hydrolase, Rhamnogalacturonan Lyase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azo-Wheat Arabinoxylan (Liquid)
| Content: | 3 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-1,4-β-Xylanase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azo-Wheat Arabinoxylan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Prepared by dyeing wheat flour arabinoxylan with Remazol Brilliant Blue R dye. Substrate for the assay of endo-1,4-β-D-xylanase.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azo-Wheat Arabinoxylan (Powder)
| Content: | 3 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-1,4-β-Xylanase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azo-Wheat Arabinoxylan for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Prepared by dyeing wheat flour arabinoxylan with Remazol Brilliant Blue R dye. Substrate for the assay of endo-1,4-β-D-xylanase.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azo-Xylan (Birchwood) (Liquid)
| Content: | 2 x 100 mL (1% w/v) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | 2-8oC |
| Physical Form: | Liquid |
| Stability: | > 1 year under recommended storage conditions |
| Substrate For (Enzyme): | endo-1,4-β-Xylanase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azo-Xylan (Birchwood) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Substrate for the specific assay of endo-1,4-β-D-xylanase.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Azo-Xylan (Birchwood) (Powder)
| Content: | 3 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-1,4-β-Xylanase |
| Assay Format: | Spectrophotometer, Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 7% |
High purity dyed, soluble Azo-Xylan (Birchwood) for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Substrate for the specific assay of endo-1,4-β-D-xylanase.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Amylazyme Tablets
| Content: | 200 Tablets or 1,000 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | α-amylase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
| Method recognition: | AACC Method 22-05.01 and RACI Standard Method |
High purity dyed and crosslinked Amylazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
For the assay of cereal and microbial α-amylase. Containing AZCL-Amylose. Recommended substrate for the assay of α-amylase in weather-damaged cereal grains, honey samples and food products containing low levels of this activity.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Amylazyme HY Tablets
| Content: | 200 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | α-amylase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
High purity dyed and crosslinked Amylazyme tablets for the measurement of diastase activity (α-amylase) in honey
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Arabinazyme Tablets
| Content: | 200 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Physical Form: | Solid |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Arabinanase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
High purity dyed and crosslinked Arabinazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
For the assay of endo-1,5-α-L-arabinanase. Containing AZCL-Debranched arabinan.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Cellazyme C Tablets
| Content: | 200 Tablets or 1,000 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 6 months under recommended storage conditions |
| Substrate For (Enzyme): | endo-Cellulase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
High purity dyed and crosslinked Cellazyme C tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
For the assay of endo-cellulase. Containing AZCL-HE-Cellulose. Recommended substrate for the assay of endo-cellulase.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Cellazyme T Tablets
| Content: | 200 Tablets or 1,000 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Cellulase, Xyloglucanase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
High purity dyed and crosslinked Cellazyme T tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
A unique substrate for the measurement of endo-cellulases active on highly branched and insoluble cellulosic substrates. Containing AZCL-Xyloglucan. Highly sensitive substrate.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
β-Glucazyme Tablets
| Content: | 200 Tablets or 1,000 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Cellulase, β-Glucanase/Lichenase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
High purity dyed and crosslinked β-Glucazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
For the assay of malt β-glucanase and endo-cellulase. Containing AZCL-Barley β-glucan.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
1,3-β-Glucazyme HS Tablets
| Content: | 200 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 10 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-1,3-β-Glucanase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
High purity dyed and crosslinked 1,3-β-Glucazyme HS tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
A high sensitivity substrate for the assay of endo-1,3-β-glucanase. Containing AZCL-curdlan. This substrate is approximately 10-times more sensitive than 1,3-β-Glucazyme.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Chitozyme Tablets
| Content: | 200 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 10 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-1,3-β-Glucanase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
High purity dyed and crosslinked 1,3-β-Glucazyme HS tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
A high sensitivity substrate for the assay of endo-1,3-β-glucanase. Containing AZCL-curdlan. This substrate is approximately 10-times more sensitive than 1,3-β-Glucazyme.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
α-Dextrazyme Tablets
| Content: | 200 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 10 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Dextranase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
High purity dyed and crosslinked α-Dextrazyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
Test tablets for the measurement of 1,6-α-Dextranase; i.e. enzymes active on 1,6-α-dextran. Containing AZCL α-dextran.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
Limit-Dextrizyme Tablets
| Content: | 200 Tablets or 1,000 Tablets |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Solid |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | Isoamylase, Isopullulanase, Neopullulanase, Pullulanase/Limit-Dextrinase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
| Reproducibility (%): | ~ 5% |
| Method recognition: | RACI Standard Method |
The T-LDZ-1000T pack size has been discontinued
High purity dyed and crosslinked Limit-Dextrizyme tablets for the measurement of enzyme activity, for research, biochemical enzyme assays and in vitro diagnostic analysis.
New updated assay protocol employing amyloglucosidase into sample preparation. For the assay of limit-dextrinase and pullulanase. Containing AZCL-Pullulan.
| Symbol : | GHS08 |
| Signal Word : | Danger |
| Hazard Statements : | H334 |
| Precautionary Statements : | P261, P284, P304+P340, P342+P311, P501 |
AZCL-Amylose
| Content: | 5 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 7 years under recommended storage conditions |
| Substrate For (Enzyme): | α-amylase |
| Assay Format: | Spectrophotometer (Semi-quantitative), Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
.
High purity dyed and crosslinked insoluble AZCL-Amylose for identification of enzyme activities in research, microbiological enzyme assays and in vitro diagnostic analysis.
Dyed and crosslinked amylose. Substrate for the assay of α-amylase.
| Content: | 5 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 7 years under recommended storage conditions |
| Substrate For (Enzyme): | α-amylase |
| Assay Format: | Spectrophotometer (Semi-quantitative), Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
High purity dyed and crosslinked insoluble AZCL-Amylose for identification of enzyme activities in research, microbiological enzyme assays and in vitro diagnostic analysis.
AZCL-Arabinan (Debranched)
| Content: | 3 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Below -10oC |
| Physical Form: | Powder |
| Stability: | > 9 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Arabinanase |
| Assay Format: | Spectrophotometer (Semi-quantitative), Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
High purity dyed and crosslinked insoluble AZCL-Arabinan (Debranched) for identification of enzyme activities in research, microbiological enzyme assays and in vitro diagnostic analysis.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
AZCL-HE-Cellulose
| Content: | 3 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 2 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Cellulase |
| Assay Format: | Spectrophotometer (Semi-quantitative), Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
High purity dyed and crosslinked insoluble AZCL-HE-Cellulose for identification of enzyme activities in research, microbiological enzyme assays and in vitro diagnostic analysis.
Substrate for the assay of endo-cellulase.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
AZCL-Xyloglucan (Tamarind)
| Content: | 4 g |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Ambient |
| Physical Form: | Powder |
| Stability: | > 10 years under recommended storage conditions |
| Substrate For (Enzyme): | endo-Cellulase, Xyloglucanase |
| Assay Format: | Spectrophotometer (Semi-quantitative), Petri-dish (Qualitative) |
| Detection Method: | Absorbance |
| Wavelength (nm): | 590 |
.
High purity dyed and crosslinked insoluble AZCL-Xyloglucan (Tamarind) for identification of enzyme activities in research, microbiological enzyme assays and in vitro diagnostic analysis.
| Symbol : | Not Applicable |
| Signal Word : | Not Applicable |
| Hazard Statements : | Not Applicable |
| Precautionary Statements : | Not Applicable |
