ARACUS Amino Acid Analyzer
ANALYTICAL, DETECTION AND QUALITY CONTROL INSTRUMENTS
ARACUS lite Amino Acid Analyzer
ARACUS lite Amino Acid Analyzer
- The ARACUS “lite” is a simplified configuration of the ARACUS “classic” and designed for a reduced number of samples and smaller available budget.
- Due to the small sample throughput and less eluent consumption the bottles of eluents are reduced in size and makes it possible to store the eluent bottles inside of the device and the rack. For analysis of 1° amino acids with 570 nm, for 1° and 2° with 500 nm.
- The system can be upgraded to a dual wavelength photometer with 440 and 570 nm by replacing the already installed photometer.
Main technical specifications of the system
- Method
- Post column derivatization with ninhydrin
- Reproducibility of retention time & area:
- < 0.3 % RSD
- ca. 1 % RSD
- Detection limit
- < 2 nmol/mL
Autosampler
The ARAUS lite can be upgraded later on with autosampler.
Prime and purge valves
High pressure side prime and purge valves in eluent and reagent flow.
IQ/OQ/PQ documents
Validation documents IQ/OQ and PQ for ARACUS.
Software 21 CFR Part 11 compliance
Manual and automatic peak integration with integrated IQ/OQ procedure for software (only available with software Clarity).
Inert gas
It supplies the main unit and eluent box with a protective gas atmosphere by reducing the presence of atmospheric oxygen to enhance the signal to noise ratio.
Dual wavelength photometer
The existing 500 or 570 nm photometer will be replaced by a photometer with 440 nm and 570 nm wavelength.
| method | post column derivatization with Ninhydrin |
| autosampler | ARACUS lite can be upgraded with autosampler; 2 racks, each of them with space for 48 vials, sample cooling dosage in 3 different injection modes: full loop (100 μL), partial loop (5 – 50 μL), microliter pickup (1 – 35 μL) |
| column | stainless steel, cation exchange resin reproducibility of retention time < 0.3 % RSD, reproducibility of peak area ~ 1.0 % RSD |
| column oven | 20 – 100 °C, accuracy 0.1 % |
| reactor temperature | 20 – 140 °C |
| spectroscopic detection | measuring range: high, low LED photometer (570 nm or 500 nm), or dual photometer (440 # 570 nm), 8 years warranty, flow cell: 10 μL volume, 3 years warranty |
| detection limit | < 40 pmol / 20 µL with Ninhydrin |
| quantification limit | > 100 pmol / 20 µL with Ninhydrin |
| pump system | single pump reproducibility of low rate: 0.1 % RSD at 100 μL/min, max. pressure: 400 bar | flow rate: 0.01 – 10.00 mL/min |
| maximum operating pressure | 120 bar |
| ready to use kits | kits including eluents, reagent and additional dilution buffer, ammonia adsorber and separation columns for 500 – 1000 runs |
| columns & eluents | Compatible with all High-Speed and High-Resolution columns, operating with all eluents based on different ionic strength (Li, Na, K) |
| control software & data aquisition | Clarity (21 CFR part 11 compliant) or AminoPeak |
| dimensions, weight, power | 1080 x 370 x 375 mm*, 60 kg, 110 – 230 V |
| *800 mm with opened main unit |
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ARACUS classic Amino Acid Analyzer
ARACUS classic Amino Acid Analyzer
- The setup of the ARACUS “classic” is the standard configuration of the analyzer series.
- The ARACUS “classic” allows the continous analysis of 96 samples in a cooled autosampler. Eluents and reagent solution are selectively chosen through valves and are ideally mixed to each other.
- The addition of ninhydrin provides a quantitative analysis statement.
- A washing routine of the injection valve and syringe pump avoids cross contaminations.
- The level of the individual reagents can be monitored during the runs and warns the user if the quantity is no longer sufficient.
Main technical specifications of the system
- Method
- Post column derivatization with ninhydrin
- Reproducibility of retention time & area
- < 0.3 % RSD
- ca. 1 % RSD
- Detection limit
- < 2 nmol/mL
Manual injection port
Upon request each system can be supplied as manual configuration.
Prime and purge valves
High pressure side prime and purge valves in eluent and reagent flow.
IQ/OQ/PQ documents
Validation documents IQ/OQ and PQ for ARACUS.
Software 21 CFR Part 11 compliance
Manual and automatic peak integration with integrated IQ/OQ procedure for software (only available with software Clarity).
Inert gas
It supplies the main unit and eluent box with a protective gas atmosphere by reducing the presence of atmospheric oxygen to enhance the signal to noise ratio.
Level control
Active control of fluid volume by weight cell of each bottle. (only available with software aminoPeak).
InVitro diagnostic kit IVDD 98/79 ED
IVD kit for the qualitative and quantitative determination of amino acids in physiological fluids like human blood and urine for medical diagnostics in combination with the ARACUS IVD classic.
| method | post column derivatization with Ninhydrin |
| autosampler | 2 racks, each of them with space for 48 vials, sample cooling dosage in 3 different injection modes: full loop (100 μL), partial loop (5 – 50 μL), microliter pickup (1 – 35 μL) |
| column | stainless steel, cation exchange resin reproducibility of retention time < 0.3 % RSD, reproducibility of peak area ~ 1.0 % RSD |
| column oven | 20 – 100 °C, accuracy 0.1 % |
| reactor temperature | 20 – 140 °C |
| spectroscopic detection | measuring range: high, low LED photometers (440 nm + 570 nm), 8 years warranty, flow cell: 10 μL volume, 3 years warranty |
| detection limit | < 40 pmol / 20 µL with Ninhydrin |
| quantification limit | > 100 pmol / 20 µL with Ninhydrin |
| pump system | single pump, reproducibility of low rate: 0.1 % RSD at 100 μL/min, max. pressure: 400 bar | flow rate: 0.01 – 10.00 mL/min |
| maximum operating pressure | 120 bar |
| ready to use kits | kits including eluents, reagent and additional dilution buffer, ammonia adsorber and separation columns for 500 – 1000 runs |
| columns & eluents | Compatible with all columns, operating with all eluents based on different ionic strength (Li, Na, K) |
| control software & data aquisition | Clarity (21 CFR part 11 compliant) or AminoPeak |
| dimensions, weight, power | 1080 x 370 x 375 mm*, 60 kg, 110 – 230 V |
| *800 mm with opened main unit |
|---|
ARACUS advanced Amino Acid Analyzer
ARACUS lite Amino Acid Analyzer
- The ARACUS “advanced” is an extended configuration of the ARACUS “classic” by an additional second pump drive.
- The system allows the continous analysis of 96 samples in a cooled autosampler.
- The upgraded pump configurations allows the independent operation of two fluidic lines (eluent side and reactive side).
- The use of two pump drives results in an improved signal-to-noise ratio of the chromatogram.
- The freely adjustable parameters between the eluent and the reagent pump drives also provide the possibility of using the reagent OPA (o-phthalaldehyde) for detection of primary amines with a fluorescence detector.
- The addition of ninhydrin provides a quantitative analysis statement.
- A washing routine of the injection valve and syringe pump avoids cross contaminations.
Main technical specifications of the system
- Post column derivatization with ninhydrin
- Reproducibility of retention time & area
- < 0.3 % RSD
- ca. 1 % RSD
- Detection limit
- < 2 nmol/mL
Manual injection port
Upon request each system can be supplied as manual configuration.
Prime and purge valves
High pressure side prime and purge valves in eluent and reagent flow.
IQ/OQ/PQ documents
Validation documents IQ/OQ and PQ for ARACUS.
Fluorescence detector
For sensitive fluorescence detection of 1° amines with o-phatalaldehyde (OPA) we are providing the suitable fluorescence detector.
Inert gas
It supplies the main unit and eluent box with a protective gas atmosphere by reducing the presence of atmospheric oxygen to enhance the signal to noise ratio.
Level control
Active control of fluid volume by weight cell of each bottle. (only available with software aminoPeak).
InVitro diagnostic kit IVDD 98/79 EC
IVD kit for the qualitative and quantitative determination of amino acids in physiological fluids like human blood and urine for medical diagnostics in combination with the ARACUS IVD advanced.
Software 21 CFR Part 11 compliance
Manual and automatic peak integration with integrated IQ/OQ procedure for software (only available with software Clarity).
| method | post column derivatization with Ninhydrin or OPA |
| autosampler | 2 racks, each of them with space for 48 vials, sample cooling dosage in 3 different injection modes: full loop (100 μL), partial loop (5 – 50 μL), microliter pickup (1 – 35 μL) |
| column | stainless steel, cation exchange resin reproducibility of retention time < 1 % RSD, reproducibility of peak area < 3.0 % RSD |
| column oven | 20 – 100 °C, accuracy 0.1 % |
| reactor temperature | 20 – 140 °C |
| spectroscopic detection | measuring range: high, low LED photometers (440 nm + 570 nm), 8 years warranty, flow cell: 10 μL volume, 3 years warranty |
| detection limit | < 40 pmol / 20 µL with Ninhydrin, < 4 pmol / 20 µL with OPA |
| quantification limit | > 100 pmol / 20 µL with Ninhydrin, > 10 pmol / 20 µL with OPA |
| pump system | dual pump reproducibility of low rate: 0.1 % RSD at 100 μL/min, max. pressure: 400 bar | flow rate: 0.01 – 10.00 mL/min |
| maximum operating pressure | 120 bar |
| ready to use kits | kits including eluents, reagent and additional dilution buffer, ammonia adsorber and separation columns for 500 – 1000 runs |
| columns & eluents | Compatible with all columns, operating with all eluents based on different ionic strength (Li, Na, K) |
| control software & data aquisition | Clarity (21 CFR part 11 compliant) or AminoPeak |
| dimensions, weight, power | 1000 x 680 x 360 mm*, 60 kg, 110 – 230 V |
| *800 mm with opened main unit |
|---|
