ADAMII™ / FLUORESCENCE STEM CELL COUNTER
LIFE SCIENCE PRODUCTS
IVD PRODUCTS
BLOOD QC PRODUCT
- ADAM™ rWBC / residual White Blood Cell Counters
- ADAMII™ / Fluorescence Stem Cell Counter
ADAMⅡ
Fluorescence CD34 Counter
– 15 minutes for counting CD34
– Easy to use
– Accurate and reliable result
– 4 channel (Bright, PerCP, PE, FITC)
– State-of-the-arts optics and image analysis software
ADAMⅡ started to release officially after receiving an approval from US FDA 510(k) on 08/09/2019 (FDA device listing Link)
Revolutionary new way to count CD34!
ADAMⅡ is an image-based fluorescence cell counter, which captures and analyzes multiple images.
It has 4-channel (Bright, Yellow (PE), Red (PerCP) and Green (FITC)) light sources.
Following specimens can be analyzed: normal and mobilized peripheral blood (PB/MPB),
as well as fresh hematopoietic progenitor cell-apheresis (HPC-A) collection.
ADAMⅡ measures the percentage of viable CD34 positive cells from total leukocytes and absolute number of CD34 positive cells (cells/μL).
Simple and easy to use
Add the sample and reagent to an empty tube, then add the lysis buffer.
Load the stained sample onto the slide and insert into the ADAMⅡ instrument to get the result.
Up to 7 results will be generated and displayed : (1) Total CD45 (cells/μL) (2) Viable CD45 cells/μL (3) Total CD34 (%, cells/μL)
(4) Viable CD34 (%, cells/μL) (5) Viable CD34 of Viable CD45 (%) (6) CD45 Viability (%) (7) CD34 Viability (%).
ADAMⅡ provides an accurate and precise results with rapid turnaround and minimal hands-on time by the operator.
The high degree of precision of the system is due in part to the simplifying sample preparation steps and
eliminating washing and reconstituting steps, which may cause cell loss and human error.
In addition, results are reported using the test specific software, limiting interpretation steps by the user.
Substantially equivalent to flow cytometry
Mobilized PB and PBSC samples were used to compare with ADAMⅡ and flow cytometry.
Refer to the comparison data, linear regression analysis revealed a close correlation
between the data for the absolute number of CD34+ and its cell fractions(%) obtained with the ADAMⅡ and the flow cytometry.
The correlation for CD34 cell counts between the ADAMⅡ and the flow cytometry
(A) Absolute number of CD34+ cells, (B) Percentage of CD34 +cells (A: Total CD34 (Cells/μL), B: Total CD34 (%))
The correlation for CD34 cell counts between the ADAMⅡ and the flow cytometry
(A) Absolute number of CD34+ cells, (B) Percentage of CD34 +cells (A : Viable CD34 (Cells/μL) B : Viable CD34 (%))
Product name / Cat. No: ADAMⅡ / ADAMⅡ
Camera: High-sensitivity monochrome CCD
Optical: Bright, Yellow (PE), Red (PerCP) and Green (FITC)
Weight: 19.3 kg
Size (LxWxH): 300 x 420 x 370 mm
Lens: Olympus lens (10X) / 35∮tube lens
Specimen: HPC-A, Mobilized PB, Control
Filter: PerCP: BLP01-635R (650 long pass)
Total frame / Total Volume: 136 frame / 7.854 uL
PE: Excitation 525/50, Emission 572/28-25
Reading range / Chamber Volume: 28 x 8 x 0.1 mm / 25 uL
FITC: Excitation 466/40, Emission 550/50
Stage: Automated X-Y-Z stage
ADAMⅡ-CD34 Kit
Disposables for ADAMⅡ
– Hematopoietic stem cell (CD34) count
– Disposable ADAMⅡ Assay Slide
– Reduces exposures to hazardous blood and contamination
CD34 Reagent contains CD34-PE antibody, CD45-perCP antibody, and Nuclear Acid Stain
for analyzing CD34 positive cells, CD45 positive cells, and cell survival respectively.
ADAMⅡ-CD34 Kit
Principles of procedure
When the sample is mixed with reagent in the test tube, fluorescence-labeled antibodies bind specifically to the CD34+ and CD45+ cell
surface and dead cells are stained by nucleic acid staining dye.
Once the staining step is completed, RBC Lysis buffer is added for the lysis of red blood cells.
The lysed sample is loaded into a disposable plastic slide and the slide is placed on the precision stage of ADAMⅡ instrument.
Product name / Cat. No:ADAMⅡ-CD34 Kit / CD34K-025
Staining solution: PE, PerCP, Nucleic acid dye
1 Box: 25 tests
Loading sample volume: 25 µL / test
ADAM-rWBC Kit
Disposables for ADAM-rWBC series
ADVANTAGE
Simple and easy to use
The ADAM-rWBC Kit consists of standard bead solution, r-solution, and r-slide.
For absolute counting of residual leukocytes, appropriate sample preparation is needed.
After mixing the sample with the provided r-Solution, load the sample into the r-Slide.
Then, the number of total leukocytes will be automatically calculated and displayed on the ADAM-rWBC series.
Product name / Cat. No: ADAM rWBC Kit / AD1K-050
Quantity: 50 tests / kit
Staining method: PI (Propidium lodide)
Sample loading volume: 100 µL
Contents: r-Slide: 50 slides / r-Solution: 25 ml x 1 bottle / Standard bead solution: 7 ml x 1 bottle
